Ermiao San Attenuating Rheumatoid Arthritis Via Pi3k/Akt/Mtor Signaling Activate Hif-1 Α Induced Glycolysis

Опубликована: Янв. 1, 2025

Ethnopharmacological relevanceAs a classic formula, Ermiao San (EMS) characterized by its less medicinal flavor and strong potency had been proven to be effective safe in the treatment of rheumatoid arthritis (RA) during clinical experience our previous research.Aim studyThe therapeutic characteristics multi-component multi-target traditional Chinese medicine prompted us further investigated compounds EMS, evaluated potential mechanisms treating RA.Materials methodsFirstly, ultra-high-performance liquid chromatography high-resolution mass spectrometry (UPLC-HRMS) was used analyse main absorption components EMS absorbed into rat serum, secondary carried assisted determining structure compounds. Then, open field experiments, H&E staining, safranin-O-turquoise ELISA, other methods were applied verify alleviating effects on exercise ability, inflammation, cartilage damage CIA rats. We established RA-FLS model using TNF-α observed cell migration invasion through wound healing transwell. In addition, immunohistochemistry western blot investigate PI3K/AKT/mTOR/HIF-1α pathway vivo vitro.ResultsIn study, 17 identified which regarded as active ingredients involved improvement RA EMS. Furthermore, showed outstanding anti-RA manifested improving foot swelling scores, pathological changes joint tissue, inhibiting inflammatory factors, restoring ability. And data that could reduce injury signaling pathway. vitro studies have shown induce expression GLUT1 HK2 proteins, accelerate glycolysis rate, promote cell, ultimately lead adverse outcomes. While regulate related molecules Glut1 pathway, thereby migration, cells.ConclusionThe excellent effect rats attributed inhibition synovial fibroblasts enriches mechanism RA.

Язык: Английский

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Analyzing How Zhizi Baipi Decoction Regulates VEGF to Suppress RA Angiogenesis Using Network Pharmacology and Experimental Validation

Phytochemical Analysis, Год журнала: 2025, Номер unknown

Опубликована: Янв. 14, 2025

Rheumatoid arthritis (RA) is a chronic autoimmune disease that primarily manifests with symptoms such as heat and toxin. However, the key components molecular mechanisms of Zhizi Baipi decoction (ZBD) in treatment RA are still unclear. The study aimed to explore mechanism action ZBD for treating through ingredient analysis, network pharmacology, experimental validation. chemical constituents were identified by ultra-high performance liquid chromatography coupled Q-TOF-mass spectrometry (UPLC-Q-TOF-MSE). Additionally, active ingredients screened pharmacology using docking verify binding energy ZBD's targets. Then we elucidated on collagen-induced (CIA) model rats. Subsequently, validations used validate findings pharmacology. A total 84 was UPLC-Q-TOF-MSE. results indicated could exert its therapeutic effect vascular endothelial growth factor (VEGF) pathway. Molecular revealed strong capacity between target KDR ingredients. quantified five ZBD. In vivo experiments demonstrated inhibited synovial angiogenesis alleviated occurrence progression RA. Overall, has significant qualitative docking, main modulate VEGF pathway treat

Язык: Английский

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Integrating Transcriptomics and Network Pharmacology to Reveal the Effect and Mechanism of Bai-Jie-Jing-Xie Ointment on Improving Skin Inflammation of Psoriasis

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119680 - 119680

Опубликована: Март 1, 2025

Язык: Английский

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Ermiao San attenuating Rheumatoid Arthritis via PI3K/AKT/mTOR signaling activate HIF-1α induced glycolysis

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119615 - 119615

Опубликована: Март 1, 2025

Язык: Английский

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Regulating Rheumatoid Arthritis From the Perspective of Metabolomics: A Comprehensive Review

International Journal of Rheumatic Diseases, Год журнала: 2025, Номер 28(3)

Опубликована: Март 1, 2025

ABSTRACT Rheumatoid arthritis (RA) is a severe inflammatory autoimmune disease with metabolic changes. RA patients have abnormalities in glycolysis, amino acid metabolism, choline and fatty synthesis. The differential metabolites individuals of animal models were explored to find the potential biomarkers for risk prediction, diagnosis, prognosis perspective metabolism. Moreover, we discussed changes related after treatment anti‐rheumatic drugs, Traditional Chinese Medicine (TCM) explore promising metabolites. In addition, immunological mechanism TCM from metabolism was also clarified. For perspectives research application beneficial clinic, relevant technologies focuses future studies field been proposed accordingly.

Язык: Английский

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Network pharmacology and transcriptomics explore the therapeutic effects of Ermiao Wan categorized formulas for diabetes in mice

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 6, 2024

Ermiao wan (EMW) is a classical traditional Chinese medicine formula, with two modified versions including Sanmiao (SMW) and Simiao (FMW). These categorized formulas (ECFs) are traditionally used to treat gouty arthritis hyperuricemia. However, their potential benefits mechanisms on diabetes remain be explored. This study aims investigate the overall effects biological differences of ECFs in high fat diet (HFD)-fed mice based network pharmacology transcriptomics. significantly reduced body weight, improved oral glucose tolerance, decreased accumulation, lowered serum insulin inflammatory cytokine levels HFD-fed mice. FMW had better efficacy than EMW SMW. Network analysis revealed that targeted functional modules associated chronic inflammation, lipid metabolism, metabolism. Transcriptome results also showed could inhibit genes inflammation upregulated some Comprehensive QPCR verification indicated beneficial might attributed regulation Ddit3, Ccl2, Esr1, Cyp7a1. provides theoretical basis for clinical use ECFs.

Язык: Английский

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Integrated metabolomics and network pharmacology to reveal the mechanisms of Processed Aurantii Fructus in the treatment of CUMS-induced functional dyspepsia

Journal of Ethnopharmacology, Год журнала: 2024, Номер 337, С. 118765 - 118765

Опубликована: Авг. 29, 2024

Язык: Английский

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Integrating Lipid Metabolomics, Serum Medicinal Chemistry, Network Pharmacology and Experimental Validation to Explore the Mechanism of Sanmiao Wan in the Treatment of Rheumatoid Arthritis

Опубликована: Янв. 1, 2024

Язык: Английский

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Integrating lipid metabolomics, serum medicinal chemistry, network pharmacology and experimental validation to explore the mechanism of Sanmiao wan in the treatment of rheumatoid arthritis

Journal of Ethnopharmacology, Год журнала: 2024, Номер unknown, С. 119295 - 119295

Опубликована: Дек. 1, 2024

Язык: Английский

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Serum pharmacochemistry combined with network pharmacology reveals the hepatotoxicity mechanism of Alangium chinense (Lour.) Harms

Journal of Ethnopharmacology, Год журнала: 2024, Номер 340, С. 119312 - 119312

Опубликована: Дек. 31, 2024

Язык: Английский

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