The active ingredients and mechanism of Zuoqing San in the treatment of sigmoid ulcerative colitis by retention enema
Journal of Complementary and Integrative Medicine,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 21, 2025
Abstract
Objectives
This
investigation
aimed
to
evaluate
the
efficacy,
key
active
ingredients,
and
mechanisms
of
Zuoqing
San
(ZQS),
a
traditional
Chinese
medicine
formula,
in
managing
sigmoid
ulcerative
colitis
(SUC).
Methods
A
cohort
126
participants
was
recruited
treated
with
ZQS
at
daily
dosage
100
mL
for
12
weeks.
The
primary
endpoint
percentage
subjects
achieving
favorable
treatment
outcomes.
Liquid
chromatography-mass
spectrometry
employed
identify
principal
ingredients
ZQS.
Network
pharmacology
utilized
predict
central
targets
its
ingredients.
Protein-protein
interaction
networks,
Gene
Ontology
enrichment
analyses,
Kyoto
Encyclopedia
Genes
Genomes
pathway
analyses
were
conducted
identified
core
targets.
Finally,
molecular
dynamics
simulations
performed.
Results
Following
weeks
therapy,
withdrawal
rate
7.93
%,
outcomes
observed
31.03
%
4
weeks,
66.37
8
(Odds
Ratio:
1.54,
95
Confidence
Interval:
0.41–1.83),
68.10
1.86,
CI:
0.32–1.27).
comprises
31
chemical
constituents.
Key
within
protein-protein
network
included
TNF,
AKT1,
IL6,
IL1β,
PTGS2,
TP53,
JUN,
MMP9,
CASP3,
HIF1A,
BCL1.
Pathway
analysis
indicated
primarily
impacts
NF-kB,
IL-17
signaling
pathways.
Molecular
simulation
results
showed
oxymatrine,
cynarin
had
higher
affinity
TNF
respectively.
Conclusions
research
elucidates
components
potential
multicomponent–multitarget–multipathway
pharmacological
mechanisms,
demonstrating
promising
therapeutic
SUC
management.
Язык: Английский
Aromatic Molecular Compatibility Attenuates Influenza Virus-Induced Acute Lung Injury via the Lung–Gut Axis and Lipid Droplet Modulation
Pharmaceuticals,
Год журнала:
2025,
Номер
18(4), С. 468 - 468
Опубликована: Март 26, 2025
Background:
Acute
lung
injury
(ALI)
is
a
major
cause
of
death
in
patients
with
various
viral
pneumonias.
Our
team
previously
identified
four
volatile
compounds
from
aromatic
Chinese
medicines.
Based
on
molecular
compatibility
theory,
we
defined
their
combination
as
(AC),
though
its
therapeutic
effects
and
underlying
mechanisms
remain
unclear.
Methods:
This
study
used
influenza
A
virus
(IAV)
A/PR/8/34
to
construct
cell
mouse
models
ALI
explore
AC’s
protective
against
infection.
The
effect
AC
was
verified
by
evaluating
the
antiviral
efficacy
models,
including
improvements
colon
inflammation,
oxidative
stress,
suppression
NLRP3
inflammasome.
In
addition,
16S
rDNA
lipid
metabolomics
were
analyze
potential
AC.
Results:
vitro
vivo
studies
demonstrated
that
increased
survival
IAV-infected
cells
mice,
inhibited
replication
expression
proinflammatory
factors
tissues,
ameliorated
barrier
damage
colonic
tissues.
ROS
inflammasome
improved
inflammatory
infiltration
into
Finally,
effectively
regulated
intestinal
flora
disorders
metabolism
model
significantly
reduced
cholesterol
triglyceride
expression,
thus
abnormal
accumulation
droplets
(LDs)
after
IAV
Conclusions:
this
study,
could
treat
IAV-induced
ALIs
through
multiple
pathways,
anti-inflammatory
pathways
modulation
LDs.
Язык: Английский
Innovative Application of Medicinal Insects: Employing UHPLC-MS, Bioinformatics, In Silico Studies and In Vitro Experiments to Elucidate the Multi-Target Hemostatic Mechanism of Glenea cantor (Coleoptera: Cerambycidae) Charcoal-Based Medicine
Pharmaceuticals,
Год журнала:
2025,
Номер
18(4), С. 479 - 479
Опубликована: Март 27, 2025
Background:
Longhorn
beetles,
a
widely
recognized
group
of
Chinese
traditional
medicinal
insects,
are
characterized
by
their
notable
hemostatic
properties.
However,
the
comprehensive
understanding
potential
has
been
hindered
limitations
current
research
methodologies.
Methods:
This
study
focuses
on
species
Glenea
cantor
(Fabricius),
which
can
produce
several
generations
per
year,
and
introduces
novel
method
using
microwave
carbonization
techniques.
By
employing
an
in
vitro
coagulation
test,
UHPLC-MS,
network
pharmacology,
molecular
docking,
dynamics
simulation,
efficacy
mechanism
action
charcoal
medicine
(GC-CM)
were
thoroughly
studied.
Results:
In
tests
showed
that
GC-CM
significantly
reduced
activated
partial
thromboplastin
time
(APTT)
prothrombin
(PT),
indicating
its
ability
to
enhance
cascade
preliminarily
confirming
(p
<
0.01
vs.
blank
control
group).
The
analysis
revealed
comprises
453
components,
including
137
bioactive
components
with
high
human
utilization.
After
predictions
via
databases
such
as
SwissTargetPrediction
deduplication,
215
targets
linked
specificity
identified.
These
regulate
signaling
pathways
platelet
activation,
complement
cascades,
cGMP-PKG.
Molecular
docking
demonstrated
strong
affinities
between
key
SRC
PIK3R1
compounds
2′,6′-dihydroxy
4′-methoxydihydrochalcone,
1-monolinoleoyl-rac-glycerol
(binding
energy
−5
kcal/mol).
simulations
show
good
binding
capacity
core
Conclusions:
aim
this
was
elucidate
material
basis
GC-CM,
offering
model
for
exploring
other
insect-based
resources.
Язык: Английский
Baicalein tethers CD274/PD-L1 for autophagic degradation to boost antitumor immunity
Autophagy,
Год журнала:
2024,
Номер
unknown, С. 1 - 17
Опубликована: Дек. 22, 2024
Immune
checkpoint
inhibitors,
especially
those
targeting
CD274/PD-L1yield
powerful
clinical
therapeutic
efficacy.
Thoughmuch
progress
has
been
made
in
the
development
of
antibody-basedCD274
drugs,
chemical
compounds
applied
for
CD274degradation
remain
largely
unavailable.
Herein,baicalein,
a
monomer
traditional
Chinese
medicine,
isscreened
and
validated
to
target
CD274
induces
itsmacroautophagic/autophagic
degradation.
Moreover,
we
demonstrate
thatCD274
directly
interacts
with
MAP1LC3B
(microtubule
associatedprotein
1
light
chain
3
beta).
Intriguingly,
baicalein
potentiatesCD274-LC3
interaction
facilitate
autophagic-lysosomal
degradationof
CD274.
Importantly,
targeted
degradation
via
baicaleininhibits
tumor
by
boosting
T-cell-mediated
antitumorimmunity.
Thus,
elucidate
critical
role
autophagy-lysosomalpathway
mediating
degradation,
conceptually
demonstratethat
design
molecular
"glue"
that
tethers
CD274-LC3interaction
is
an
appealing
strategy
develop
inhibitors
incancer
therapy.
Язык: Английский