d‐Amphetamine and Feeding States Cohesively Affect Locomotion and Motor Neuron Response in Zebrafish Larvae DOI Creative Commons
Pushkar Bansal, Mitchell F. Roitman, Erica E. Jung

и другие.

Brain and Behavior, Год журнала: 2024, Номер 14(12)

Опубликована: Дек. 1, 2024

ABSTRACT Purpose Amphetamine (AMPH) increases locomotor activities in animals, and the response to AMPH is further modulated by caloric deficits such as food deprivation restriction. The increment activity regulated AMPH‐caloric deficit concomitance can be varying feeding schedules (e.g., acute chronic after deprivation). However, effects of different on AMPH‐induced are yet explicated. Here, we have explored stimulatory responses acutely administered D‐amphetamine locomotion under systematically states (fed/sated deprivation) (chronic acute) zebrafish larvae. Method We exposed wild‐type transgenic [Tg(mnx1:GCaMP5)] larvae 0.7 µM concentration measured swimming spinal motor neuron vivo real time. analysis involved time‐elapsed cumulative manner pre‐ post‐AMPH treatment four including hunger. Both were compared all both fish lines. Findings Our results show that increased cumulatively. A steady increase was observed an immediate abrupt food‐deprivation state. ad libitum‐fed exhibited a moderate activity. Conversely other states, food‐sated (acute moved moderately less mild decrease treatment. Conclusion These reveal importance cohesive schedule revealing changes characteristics states.

Язык: Английский

Development of a trace quantitative method to investigate caffeine distribution in the Yellow and Bohai Seas, China, and assessment of its potential neurotoxic effect on fish larvae DOI
Yan Zhi, Yaxi Li,

Ainuo Lin

и другие.

Marine Pollution Bulletin, Год журнала: 2023, Номер 195, С. 115492 - 115492

Опубликована: Сен. 8, 2023

Язык: Английский

Процитировано

4
Modulation of locomotion and motor neuron response by the cohesive effect of acute and chronic feeding states and acute d-amphetamine treatment in zebrafish larvae DOI Open Access
Pushkar Bansal, Mitchell F. Roitman, Erica E. Jung

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 30, 2024

Abstract Amphetamine (AMPH) increases locomotor activities in animals, and the response to AMPH is further modulated by caloric deficits such as food deprivation restriction. The increment activity regulated AMPH-caloric deficit concomitance can be varying feeding schedules (e.g. acute chronic after deprivation). However, effects of different on AMPH-induced are yet explicated. Here, we have explored stimulatory responses acutely administered d-amphetamine locomotion under systematically states (fed/sated deprivation) (chronic acute) zebrafish larvae. We used wild-type transgenic[Tg(mnx1:GCaMP5)] larvae measured swimming spinal motor neuron vivo real-time time-elapsed cumulative manner pre- post-AMPH treatment. Our results showed that increased both treatment cumulatively. A steady increase was observed food-deprivation compared an immediate abrupt state. ad libitum-fed exhibited a moderate activity. Conversely all other states, food-sated (acute moved moderately less mild decrease These point importance schedule modulating amphetamine’s characteristic behavior neurons.

Язык: Английский

Процитировано

1
Quantifying Variability in Zebrafish Larvae Locomotor Behavior across Experimental Conditions: A Learning-Based Tracker DOI Creative Commons
Zhuo Zhang,

Xinyu Chai,

Guoning Si

и другие.

Fishes, Год журнала: 2024, Номер 9(6), С. 193 - 193

Опубликована: Май 23, 2024

This study investigated the effects of environmental changes on zebrafish larval behavior, using single-factor and orthogonal experiments to assess locomotion during temperature pH changes. In experiments, larvae were exposed variations in (22 30 °C) levels (6.0, 7.0, 9.0). The simultaneous by tests. both each larva was recorded three 5 min videos at different stages (before exposure, short-term exposure (10 min), after long-term (60 min)). You Look Only Once (YOLOv5) Deep Simple Online Real Time Tracking (DeepSORT) models adopted develop a tracking system, YOLOv5 improved two aspects anchor clustering network structure. accuracy system for small targets effectively improved, reaching more than 98% MOTA (Multiple Object Accuracy). Principal Component Analysis (PCA) employed extract behavioral features from 13 motion parameters, namely activity, edge direction preference. Our findings reveal that lower temperatures acidic conditions led decrease former also increased behavior. Conversely, elevated alkaline had muted impact these behaviors. Interestingly, concurrent significantly altered directional Additionally, we observed elicited distinct temporal patterns constant level. summary, recommend precise control explicit reporting ambient breeding devices experimental wells minimize behavior enhance experiment repeatability reliability.

Язык: Английский

Процитировано

1
Hypothalamic dopamine neuron activity is modulated by caloric states and amphetamine abuse in zebrafish larvae DOI Open Access
Pushkar Bansal, Erica E. Jung

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 2, 2024

Abstract Dopaminergic (DA) neuron activity is affected by reward and stress-inducing entities such as food drugs of abuse, different DA populations can respond distinctly to these stimuli. Interaction between both stimuli significantly alters the dynamics release in populations. Additionally, stimulating affect interconnections among impairing their correspondence with each other. However, limited studies have been performed that could point effect interaction AMPH caloric states on neurons inter-correlation. This study explores individual interactive two states, ad-libitum fed (AL) deprived (FD), acute exposure a stimulant drug (amphetamine) hypothalamus zebrafish larvae. We used transgenic line Tg(th2:GCaMP7s), which expresses calcium indicator (GCaMP7s) A11(Posterior Tuberculum) part A14 (Caudal Hypothalamus Intermediate Hypothalamus) located larval zebrafish. The larvae were subjected FD ad AL feeding followed treatment 0.7uM 1.5uM doses AMPH. recorded quantified fluorescence change, duration, peak rise/fall time, latency spikes neurons. Our results show baseline amplitude, spike correlation inter- intra-DA higher than state. Dose-dependent further increased intensity aforementioned parameters dose-dependently impaired These suggest brain exhibit similar trend response AMPH, where AMPH-mediated change was dose-dependent.

Язык: Английский

Процитировано

0
Modulation of Hypothalamic Dopamine Neuron Activity by Interaction Between Caloric State and Amphetamine in Zebrafish Larvae DOI Creative Commons
Pushkar Bansal, Mitchell F. Roitman, Erica E. Jung

и другие.

Journal of Neuroscience Research, Год журнала: 2024, Номер 102(11)

Опубликована: Ноя. 1, 2024

ABSTRACT Dopamine (DA) signaling is evoked by both food and drugs that humans come to abuse. Moreover, physiological state (e.g., hunger versus satiety) can modulate the response. However, there great heterogeneity among DA neurons. Limited studies have been performed could resolve interaction between drug responsivity across groups of Here, we measured activity neurons in transgenic Tg (th2:GCaMP7s) zebrafish larva expresses a calcium indicator (GCaMP7s) A11 (posterior tuberculum) part A14 (caudal hypothalamus intermediate hypothalamus) populations located larval zebrafish. Fish were recorded one two states: ad‐libitum fed (AL) deprived (FD) before after acute exposure different doses stimulant amphetamine (0, 0.7, 1.5 μM). We quantified fluorescence change, duration, peak rise/fall time, latency spikes Our results show baseline neuron amplitude, spike correlation inter‐ intra‐DA higher FD than AL state. Dose‐dependent AMPH treatment further increased intensity these parameters but only The relatively post‐AMPH treatment. Given increases reactivity probability relapse seeking, support as potential critical mediators reinforcement.

Язык: Английский

Процитировано

0
d‐Amphetamine and Feeding States Cohesively Affect Locomotion and Motor Neuron Response in Zebrafish Larvae DOI Creative Commons
Pushkar Bansal, Mitchell F. Roitman, Erica E. Jung

и другие.

Brain and Behavior, Год журнала: 2024, Номер 14(12)

Опубликована: Дек. 1, 2024

ABSTRACT Purpose Amphetamine (AMPH) increases locomotor activities in animals, and the response to AMPH is further modulated by caloric deficits such as food deprivation restriction. The increment activity regulated AMPH‐caloric deficit concomitance can be varying feeding schedules (e.g., acute chronic after deprivation). However, effects of different on AMPH‐induced are yet explicated. Here, we have explored stimulatory responses acutely administered D‐amphetamine locomotion under systematically states (fed/sated deprivation) (chronic acute) zebrafish larvae. Method We exposed wild‐type transgenic [Tg(mnx1:GCaMP5)] larvae 0.7 µM concentration measured swimming spinal motor neuron vivo real time. analysis involved time‐elapsed cumulative manner pre‐ post‐AMPH treatment four including hunger. Both were compared all both fish lines. Findings Our results show that increased cumulatively. A steady increase was observed an immediate abrupt food‐deprivation state. ad libitum‐fed exhibited a moderate activity. Conversely other states, food‐sated (acute moved moderately less mild decrease treatment. Conclusion These reveal importance cohesive schedule revealing changes characteristics states.

Язык: Английский

Процитировано

0