Promoter of Vegetable Pea PsPIP2-4 Responds to Abiotic Stresses in Transgenic Tobacco DOI Open Access

Zhijuan Feng,

Na Liu, Yuanpeng Bu

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13574 - 13574

Опубликована: Дек. 18, 2024

Plasma membrane intrinsic proteins (PIPs), one sub-family of aquaporins (AQPs), are responsible for plant abiotic stress responses. However, little information is currently available about the responsiveness PIP promoter in vegetable pea. In present study, novel PsPIP2-4 which shared high similarity to PIP2-type AQPs from other plants, was isolated. Quantitative real-time PCR (qRT-PCR) assays suggested that predominantly expressed leaves and abundantly induced by treatments (polyethylene glycol (PEG) 6000, NaCl, methyl jasmonate (MeJA)). Further, activity verified transgenic tobacco plants. Beta-glucuronidase (GUS) staining driven confirmed it mainly detected seedlings, especially guard cells. Exposure seedlings various environmental stimuli proved strengthened osmotic, salt, MeJA stresses. This research provides stress-inducible enabling targeted gene expression under stresses demonstrates its usefulness genetic improvement resistance.

Язык: Английский

PagSND1‐B1 Regulates Wood Formation by Influencing Phosphorus Absorption and Distribution in Poplar DOI Open Access
Pu‐Jen Cheng,

Liling Gong,

Qiuxian Bai

и другие.

Plant Cell & Environment, Год журнала: 2025, Номер unknown

Опубликована: Янв. 28, 2025

ABSTRACT In natural environments, the growth and development of trees are continuously affected by phosphorus (P) starvation stress. However, mechanisms through which balance stem P distribution remain unknown. This study found that in woody model species poplar, loss stems is more severe than roots leaves under conditions, thereby inhibiting reducing expression numerous genes related to wood formation, including PagSND1‐B1 . Intriguingly, overexpression poplar enhances resistance promotes xylem development. Further analysis demonstrated can directly positively regulate transporter PagPHT1;5a Analysis content changes leaves, transgenic before after treatment indicated disrupts normal redistribution procedure, leading increased accumulation stems, beneficial for Therefore, participates absorption homoeostasis modulating provides valuable insights into regulatory function formation process

Язык: Английский

Процитировано

0
Forest Tree Breeding Under the Global Environmental Change: Challenges and Opportunities DOI Creative Commons
Chenglin Li,

B.B. Li,

Wenxuan Zhao

и другие.

Trees Forests and People, Год журнала: 2025, Номер 20, С. 100867 - 100867

Опубликована: Апрель 23, 2025

Язык: Английский

Процитировано

0
Bioengineered poplar fibres via PagGLR2.8 editing: A synergistic design for high‐performance biocomposites DOI Creative Commons
Yi An, Shiqi Wang,

Xuemin Jia

и другие.

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Апрель 29, 2025

Summary The urgent need to replace petroleum‐derived materials with sustainable alternatives drives innovation at the nexus of plant biotechnology and science. Here, we engineered Populus alba × P. glandulosa ‘84 K’ through CRISPR‐Cas9‐mediated knockout PagGLR2.8 , a glutamate receptor gene regulating vascular development, investigate its role in fibre biosynthesis composite performance. Knockout improved quality poplar by altering structure development mode tissue. Our study established relationship between quantity performance polylactic acid (PLA) composites. mechanical fire‐resistance properties these transgenic fibres/PLA composites significantly outperformed those pure PLA, demonstrating potential phloem fibres reinforce toughened Notably, also evaluated flammability dripping behaviours, findings indicating that our optimised fibre/PLA exhibit superior strengths, modulus, fire resistance, anti‐dripping, surpassing PLA. This research unveils groundbreaking approach genetic manipulation highlights promising plant‐derived enriching forest resources advancing utilisation polymers.

Язык: Английский

Процитировано

0
Success and limitations in adaptation of Fast-TrACC tissue culture-independent transformation in coffee, cotton, and tree tobacco DOI Creative Commons
Boaz Negin,

Laura Daniela Martin-Lesmes,

Georg Jander

и другие.

PLoS ONE, Год журнала: 2025, Номер 20(5), С. e0318324 - e0318324

Опубликована: Май 15, 2025

Plant transformation is a critical process for generating transgenic and genome-edited plants use in research agriculture. For most plant species, this has traditionally involved genomic insertion of DNA tissue culture regeneration transformed through hormonal induction. Recently, methods culture-independent manner, the expression growth regulators, have been published. We attempted to adapt promising approach three woody coffee ( Coffea arabica ), cotton Gossypium hirsutum tree tobacco Nicotiana glauca using combination Agrobacterium strains, plasmid systems, different promoters driving ZmWUS2 AtIPT , which were originally adapted purpose benthamiana . found that was amenable but had difficulty developing seeds. Coffee not receptive method, callus formation did exhibit gene insertions newly-formed shoots. These limitations are partially technical, such as maize WUS affecting similarly other plants, part fundamental setbacks regulators drive transformation. suggest how these drawbacks can be overcome future inducible or tissue-specific means.

Язык: Английский

Процитировано

0
Efficient In Vitro Propagation of Turpinia arguta and Quantitative Analysis of Its Ligustroflavone and Rhoifolin Content DOI Creative Commons

Jiangmei Hu,

Junhuo Cai,

Xinrui Hu

и другие.

Horticulturae, Год журнала: 2024, Номер 10(6), С. 587 - 587

Опубликована: Июнь 4, 2024

Turpinia arguta is an excellent medicinal plant mainly used for the treatment of pharyngitis, tonsillitis, and tonsillar abscesses. However, efficient regeneration protocol using tissue cultures T. does not exist. Its main constituents are flavonoids, particularly ligustroflavone rhoifolin. Here, we aimed to establish a culture system first time annual stem segments with axillary buds harvested from field Jiangxi Academy Forestry as explants by dynamically determining accumulation effective functional components in plantlets. Orthogonal tests were conducted compare effects different explant disinfection times, media, exogenous hormone ratios on induction bud growth, successional proliferation, rooting segments. The best effect was achieved disinfecting 75% ethanol 50 s, followed 0.1% mercuric chloride (HgCl2) 6 min, optimal media proliferation Murashige Skoog (MS) + 0.2 mg/L 6-benzyladenine (6-BA), 0.03 naphthaleneacetic acid (NAA), ½ MS 2.5 indole-3-butyric 0.5 NAA, respectively. detection rhoifolin plantlets 0, 3, 5 months after transplanting showed significant increasing trend eventually exceeded content requirements 2020 Edition ofChinese Pharmacopoeia arguta. Our findings provide, time, arguta, thereby providing important information support germplasm preservation, innovation, application future.

Язык: Английский

Процитировано

2
SUPERMAN Targets PHRAGMOPLAST ORIENTING KINESIN to Negatively Regulate Leaf Cell Division in Poplar DOI
Cheng Jiang,

Chunyan Zhao,

Hesheng Gao

и другие.

Plant Cell & Environment, Год журнала: 2024, Номер unknown

Опубликована: Сен. 22, 2024

Plant organs achieve their specific size and shape through the coordination of cell division expansion, processes that are profoundly influenced by environmental cues. Cytokinesis during depends on position cytokinetic wall, but how this process responses to environment fluctuations remains underexplored. Here, we investigated a regulatory module involving C2H2-type zinc finger protein (C2H2-ZFP) in leaf morphology drought stress. A total 123 C2H2-ZFP members were identified comparative genome survey Populus alba × P. glandulosa '84K'. Among them, PagSUPa, an orthologous gene Arabidopsis SUPERMAN, was selected due its responsiveness stress further confirmed play role development. Phenotypic characterization cellular analysis revealed PagSUPa fine-tunes duration proliferation adaxial epidermis, thereby influencing modulating adaxial-abaxial polarity. Additionally, found directly suppresses expression PHRAGMOPLAST ORIENTING KINESIN1 (PagPOK1) PagPOK2, genes encoding proteins involved phragmoplast orientation position, which results impaired cytokinesis wall organization. This study provides novel insights into network governed SUP development, specifically relation division.

Язык: Английский

Процитировано

2
Editorial: Tree Biology: From Genomics to Genetic Improvement DOI
Jinbo Shen, Wan-Feng Li, Chang Liu

и другие.

Plant Science, Год журнала: 2024, Номер 350, С. 112315 - 112315

Опубликована: Ноя. 10, 2024

Язык: Английский

Процитировано

0
Promoter of Vegetable Pea PsPIP2-4 Responds to Abiotic Stresses in Transgenic Tobacco DOI Open Access

Zhijuan Feng,

Na Liu, Yuanpeng Bu

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13574 - 13574

Опубликована: Дек. 18, 2024

Plasma membrane intrinsic proteins (PIPs), one sub-family of aquaporins (AQPs), are responsible for plant abiotic stress responses. However, little information is currently available about the responsiveness PIP promoter in vegetable pea. In present study, novel PsPIP2-4 which shared high similarity to PIP2-type AQPs from other plants, was isolated. Quantitative real-time PCR (qRT-PCR) assays suggested that predominantly expressed leaves and abundantly induced by treatments (polyethylene glycol (PEG) 6000, NaCl, methyl jasmonate (MeJA)). Further, activity verified transgenic tobacco plants. Beta-glucuronidase (GUS) staining driven confirmed it mainly detected seedlings, especially guard cells. Exposure seedlings various environmental stimuli proved strengthened osmotic, salt, MeJA stresses. This research provides stress-inducible enabling targeted gene expression under stresses demonstrates its usefulness genetic improvement resistance.

Язык: Английский

Процитировано

0