Genome-Wide Identification, Functional Characterization, and Stress-Responsive Expression Profiling of Subtilase (SBT) Gene Family in Peanut (Arachis hypogaea L.) DOI Open Access

Shipeng Li,

Huiwen Fu,

Yasir Sharif

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13361 - 13361

Опубликована: Дек. 13, 2024

Subtilases (SBTs), known as serine proteases or phytoproteases in plants, are crucial enzymes involved plant development, growth, and signaling pathways. Despite their recognized importance other species, information regarding functional roles cultivated peanut (Arachis hypogea L.) remains sparse. We identified 122 AhSBT genes the STQ genome, classifying them into six subgroups based on phylogenetic analysis. Detailed structural motif analyses revealed presence of conserved domains, highlighting evolutionary conservation AhSBTs. The collinearity results indicate that A. SBT gene family has 17, 5, 1 homologous pairs with Glycine max, Arabidopsis thaliana, Zea mays, respectively. Furthermore, prediction cis-elements promoters indicates they mainly associated hormones abiotic stress. GO KEGG showed many AhSBTs important stress response. Based transcriptome datasets, some genes, such AhSBT2, AhSBT18, AhSBT19, AhSBT60, AhSBT102, AhSBT5, AhSBT111, AhSBT113, remarkably higher expression diverse tissues/organs, i.e., embryo, root, leaf, potentially implicating seed development. Likewise, only a few including AhSBT1, AhSBT39, AhSBT53, AhSBT92, AhSBT115, were upregulated under (drought cold) phytohormone (ethylene, abscisic acid, paclobutrazol, brassinolide, salicylic acid) treatments. Upon inoculation Ralstonia solanacearum, levels AhSBT50, AhSBT115 disease-resistant downregulated disease-susceptible varieties. qRT-PCR-based profiling presented parallel trends generated from datasets. comprehensive dataset study provides valuable insights understanding AhSBTs, paving way for potential applications crop improvement. These findings deepen our molecular biology offer new strategies enhancing tolerance agronomically traits.

Язык: Английский

The role of the sucrose synthase gene in promoting thorn occurrence and vegetative growth in Lycium ruthenicum DOI
Wenhui Liu,

Weiman Xu,

Yue Gao

и другие.

Plant Molecular Biology, Год журнала: 2025, Номер 115(1)

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1
Genome-wide identification of R2R3-MYB transcription factor subfamily genes involved in salt stress in rice (Oryza sativa L.) DOI Creative Commons

Hao-Cheng Zhang,

Yuan-Hang Gong,

Tao Tao

и другие.

BMC Genomics, Год журнала: 2024, Номер 25(1)

Опубликована: Авг. 23, 2024

R2R3-MYB transcription factors belong to one of the largest gene subfamilies in plants, and they are involved diverse biological processes. However, role factor subfamily genes response rice (Oryza sativa L.) salt stress has been rarely reported. In this study, we performed a genome-wide characterization expression identification genes. We identified total 117 characterized their structure, chromosomal location, cis-regulatory elements. According phylogenetic relationships amino acid sequence homologies, were divided into four groups. qRT-PCR showed that levels 10 significantly increased after 3 days 0.8% NaCl treatment. selected high OsMYB2-115 for further analysis. was highly expressed roots, stem, leaf, leaf sheath. found be localized nucleus, yeast hybrid assay transcriptional activation activity. This result provides important information functional analyses related reveals may an associated with tolerance rice.

Язык: Английский

Процитировано

4
Physiological and transcriptomic analyses revealed the alleviating effects of exogenous Ca2+ and NO compound treatment on high salt stress in Reaumuria soongorica DOI Creative Commons
Zehua Liu, Hanghang Liu,

Bingbing Tan

и другие.

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Фев. 22, 2025

Soil salinization represents the most prevalent abiotic stress, severely impacting a severe impact on plant growth and crop yield. Consequently, delving into mechanism through which exogenous substances enhance salt tolerance holds significant importance for stabilization augmentation of In this study, within context seedlings R. soongorica were subjected to Ca2+ NO treatments. The aim was comprehensively explore alleviation effects high stress endured by from perspectives physiology transcriptomics. experimental results demonstrated that combined treatment increased relative water content free during conditions. Simultaneously, it induced reduction in leaf sap concentration, potential, saturation deficit, ratio bound water. These modifications effectively regulated metabolism mitigated physiological drought stress. addition, concurrent could diminish Na+ Cl− levels under At same time, effective elevating contents K+ Ca2+, thereby facilitating adjustment ion equilibrium. As result, served relieve toxicity precipitated is crucial maintaining homeostasis viability seedlings. Transcriptional analysis revealed 65 differentially expressed genes (DEGs) observable at three distinct time points Additionally, 154 DEGs detected treatment. KEGG enrichment phenylpropanoids biosynthesis, hormone signal transduction, MAPK signalling pathway, brassinosteroid biosynthesis zeatin significantly enriched compound Furthermore, WGCNA uncovered multiple genes, including ADK, SBT, F-box protein, MYB, ZIP, PAL, METTL, LRR, implicated adaptive mitigating mechanisms associated with modulating outcomes study are highly conducive disclosing ameliorates both transcriptional aspects. It also paves solid theoretical groundwork employment biotechnology breeding soongorica, offering valuable insights scientific basis further research practical applications enhancing plant's ability withstand development more salt-tolerant varieties soongorica.

Язык: Английский

Процитировано

0
Processing of plant peptide signals: critical steps mediated by subtilisins DOI

Zhichao Deng,

Yalun Yang,

Prince Marowa

и другие.

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Genome-wide identification and characterization of Subtilisin-like Serine protease encoding genes in Vigna radiata L. Wilczek DOI Creative Commons

Poornima Singh,

Brijesh Pandey, Shalini Purwar

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 17, 2025

Subtilisin-like serine proteases (SBTs) are proteolytic enzymes that play various roles in plant growth, function and stress responses. Vigna glabrescens, a wild relative of mungbean known to be potential donor photo- thermoperiod insensitivity, was characterized for thermotolerance through reproductive biology gene expression profiling. Whole-genome sequencing this species has not yet been performed; hence, genome-wide analysis explored. In the present study, systematic SBT-encoding genes V. radiata (Vradi_SBT) genome conducted, with focus on their response during flower development under different temperature regimes, such as optimum temperature, heat cold stresses, relative, glabrescens. Thirty-eight Vradi_SBT were identified further grouped into five distinct subgroups. The key domain SBT peptidase, "peptidase_S8_53," found all 38 proteins, while 28 them contained "peptidase_S8" domain. Additionally, 30 these proteins maximum 10 motifs. A total 22 orthologous Arabidopsis thaliana, whereas paralogous pairs detected tandemly duplicated unguiculata. Cis-acting element revealed presented more stress-responsive promoter sequences than other promoters. Furthermore, Vradi_SBT-1.9 significantly upregulated both high- low-temperature stresses. This study provides insights possible role species.

Язык: Английский

Процитировано

0
Characterization of Glycosyltransferase Family 1 (GT1) and Their Potential Roles in Anthocyanin Biosynthesis in Maize DOI Open Access

Huangai Li,

Yiping Li, Xiaofang Wang

и другие.

Genes, Год журнала: 2023, Номер 14(11), С. 2099 - 2099

Опубликована: Ноя. 18, 2023

Glycosyltransferase family 1 (GT1) is a large group of proteins that play critical roles in secondary metabolite biosynthesis plants. However, the GT1 not well studied maize. In this study, 107 unigenes were identified maize reference genome and classified into 16 groups according to their phylogenetic relationship. GT1s are unevenly distributed across all ten chromosomes, occurring as gene clusters some chromosomes. Collinearity analysis revealed duplication events, whole-genome or segmental duplication, tandem occurred at similar frequency, indicating both types notable expansion family. Expression showed expressing tissues with specific expression patterns each GT1, suggesting they might participate multiple biological processes during whole growth development stages. Furthermore, have those anthocyanidin synthase (ANS), enzyme anthocyanin biosynthesis. Molecular docking was carried out examine affinity substrates This study provides valuable information on will promote research functions other metabolites.

Язык: Английский

Процитировано

5
Phosphoproteomic analysis of distylous Turnera subulata identifies pathways related to endoreduplication that correlate with reciprocal herkogamy DOI Creative Commons
Paige M. Henning, Benjamin B. Minkoff, Michael R. Sussman

и другие.

American Journal of Botany, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 17, 2024

A multi-omic approach was used to explore proteins and networks hypothetically important for establishing filament dimorphisms in heterostylous Turnera subulata (Sm.) as an exploratory method identify genes future empirical research.

Язык: Английский

Процитировано

1
Genome-Wide Identification, Functional Characterization, and Stress-Responsive Expression Profiling of Subtilase (SBT) Gene Family in Peanut (Arachis hypogaea L.) DOI Open Access

Shipeng Li,

Huiwen Fu,

Yasir Sharif

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13361 - 13361

Опубликована: Дек. 13, 2024

Subtilases (SBTs), known as serine proteases or phytoproteases in plants, are crucial enzymes involved plant development, growth, and signaling pathways. Despite their recognized importance other species, information regarding functional roles cultivated peanut (Arachis hypogea L.) remains sparse. We identified 122 AhSBT genes the STQ genome, classifying them into six subgroups based on phylogenetic analysis. Detailed structural motif analyses revealed presence of conserved domains, highlighting evolutionary conservation AhSBTs. The collinearity results indicate that A. SBT gene family has 17, 5, 1 homologous pairs with Glycine max, Arabidopsis thaliana, Zea mays, respectively. Furthermore, prediction cis-elements promoters indicates they mainly associated hormones abiotic stress. GO KEGG showed many AhSBTs important stress response. Based transcriptome datasets, some genes, such AhSBT2, AhSBT18, AhSBT19, AhSBT60, AhSBT102, AhSBT5, AhSBT111, AhSBT113, remarkably higher expression diverse tissues/organs, i.e., embryo, root, leaf, potentially implicating seed development. Likewise, only a few including AhSBT1, AhSBT39, AhSBT53, AhSBT92, AhSBT115, were upregulated under (drought cold) phytohormone (ethylene, abscisic acid, paclobutrazol, brassinolide, salicylic acid) treatments. Upon inoculation Ralstonia solanacearum, levels AhSBT50, AhSBT115 disease-resistant downregulated disease-susceptible varieties. qRT-PCR-based profiling presented parallel trends generated from datasets. comprehensive dataset study provides valuable insights understanding AhSBTs, paving way for potential applications crop improvement. These findings deepen our molecular biology offer new strategies enhancing tolerance agronomically traits.

Язык: Английский

Процитировано

1