Insights into lncRNA-mediated regulatory networks in Hevea brasiliensis under anthracnose stress DOI Creative Commons

Yanluo Zeng,

Tianbin Guo,

Liping Feng

и другие.

Plant Methods, Год журнала: 2024, Номер 20(1)

Опубликована: Дек. 5, 2024

In recent years, long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) have emerged as critical regulators in plant biology, governing complex gene regulatory networks. the context of disease resistance Hevea brasiliensis, rubber tree, significant progress has been made understanding its response to anthracnose disease, a serious threat posed by fungal pathogens impacting global tree cultivation latex quality. While advances achieved unraveling genetic molecular foundations underlying resistance, gaps persist comprehending roles lncRNAs miRNAs under such stress conditions. The specific contributions these orchestrating responses against H. brasiliensis remain unclear, necessitating further exploration uncover strategies that increase resistance. Here, we integrate lncRNA sequencing, miRNA degradome sequencing decipher landscape stress. We investigated genomic profiles differentially expressed (DE-lncRNAs) constructed competitive endogenous RNA (ceRNA) network pathogenic infection. Additionally, elucidated functional HblncRNA29219 antisense hbr-miR482a, well miR390-TAS3-ARF pathway, enhancing These findings provide valuable insights into plant-microbe interactions hold promising implications for advancing agricultural crop protection strategies. This comprehensive analysis sheds light on RNA-mediated mechanisms pathogen stress, establishing foundation innovative approaches aimed at resilience sustainability agriculture.

Язык: Английский

ncRNAs in plant development and stress responses DOI
Sarvajeet Singh Gill, Nafees A. Khan, Niraj Agarwala

и другие.

Plant Physiology and Biochemistry, Год журнала: 2024, Номер 214, С. 108950 - 108950

Опубликована: Июль 18, 2024

Процитировано

6
Deciphering the Vulnerability of Pollen to Heat Stress for Securing Crop Yields in a Warming Climate DOI
Neeta Lohani, Mohan B. Singh, Prem L. Bhalla

и другие.

Plant Cell & Environment, Год журнала: 2024, Номер unknown

Опубликована: Дек. 25, 2024

ABSTRACT Climate change is leading to more frequent and severe extreme temperature events, negatively impacting agricultural productivity threatening global food security. Plant reproduction, the process fundamental crop yield, highly susceptible heatwaves, which disrupt pollen development ultimately affect seed‐set yields. Recent research has increasingly focused on understanding how grains from various crops react heat stress at molecular cellular levels. This surge in interest over last decade been driven by advances genomic technologies, such as single‐cell RNA sequencing, holds significant potential for revealing underlying regulatory reprogramming triggered throughout stages of development. review focuses affects gene networks, including response, unfolded protein autophagy, discusses impact these changes It highlights selection a key strategy improving tolerance leveraging genetic variability among grains. Additionally, genome‐wide association studies population screenings have shed light underpinnings traits major that respond high temperatures during male reproductive stages. Gene‐editing tools like CRISPR/Cas systems could facilitate precise modifications boost resilience. The information covered this valuable selecting employing approaches develop heat‐tolerant genotypes.

Язык: Английский

Процитировано

4
Elucidating circRNA-miRNA-mRNA competing endogenous regulatory RNA network during leaf rust pathogenesis in wheat (Triticum aestivum L.) DOI

Umang Kumar Dhandhanya,

Uzma Afreen, Shailendra K. Jha

и другие.

Functional & Integrative Genomics, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 16, 2025

Язык: Английский

Процитировано

0
Exploring the critical role of non-coding RNAs in plant development and stress adaptation-current status and insights DOI
Nilesh Shirish Wagh,

Pranav Amane,

R. V. Kshirsagar

и другие.

Progress in molecular biology and translational science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Small Interfering RNAs as Critical Regulators of Plant Life Process: New Perspectives on Regulating the Transcriptomic Machinery DOI Open Access
Marta Puchta, Paulina Bolc, Aleksandra Pietrusińska

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1624 - 1624

Опубликована: Фев. 14, 2025

Small interfering RNAs (siRNAs) are a distinct class of regulatory in plants and animals. Gene silencing by small is one the fundamental mechanisms for regulating gene expression. siRNAs critical regulators during developmental processes. have similar structures functions to but derived from double-stranded RNA may be involved directing DNA methylation target sequences. less well-studied than miRNA group, researchers continue identify new classes that appear at specific stages particular tissues, revealing more complex mode siRNA action previously thought. This review characterizes their biogenesis process focuses on presenting known regulation plant development responses biotic abiotic stresses. The also highlights exciting potential future research this field, proposing methods detecting bioinformatic pathway identifying functions.

Язык: Английский

Процитировано

0
Overexpression of lncRNA22524 from Dongxiang Wild Rice Reduces Drought and Salt Stress Tolerance in Cultivated Rice DOI Creative Commons
Yong Chen, Yingying Mao,

Xie Hong

и другие.

Rice, Год журнала: 2025, Номер 18(1)

Опубликована: Март 25, 2025

Drought and salt stresses are major challenges to rice production, a deep understanding of the mechanisms for tolerance could help deal with challenges. Long non-coding RNAs (lncRNAs) play crucial roles in gene regulation. Previously, lncRNA22524 has been identified as drought stress-responsive lncRNA from Dongxiang wild (DXWR). Nevertheless, its reactions abiotic genetics physiology remained unclear. In this study, we employed rapid amplification cDNA ends (RACE) obtain full-length DXWR, analyzed cellular localization, built an overexpression vector generate transgenic lines cultivated evaluated impact physiology. After treated stress, overexpressed exhibited much more injuries lower rates survival, reactive oxygen species (ROS) malondialdehyde (MDA), antioxidant enzymes proline (Pro) soluble sugar (SS) than their wild-type (WT). Furthermore, transcriptome analysis weaker WT revealed 1,233 differentially expressed genes (DEGs), where most DEGs were involved phenylpropanoid biosynthesis, photosynthesis glutathione metabolism. These findings demonstrated that negatively regulated responses which clear way working transcription metabolic products should be worth further study.

Язык: Английский

Процитировано

0
Drought stress memory in maize: understanding and harnessing the past for future resilience DOI
Latif Ahmad Peer, Aijaz A. Wani, Ajaz A. Lone

и другие.

Plant Cell Reports, Год журнала: 2025, Номер 44(5)

Опубликована: Апрель 25, 2025

Язык: Английский

Процитировано

0
Pre-mRNA Splicing Functions in Plant Sexual Reproduction Development DOI Creative Commons

Dalei Shao,

Xin‐Qi Gao, Yi Wei

и другие.

Plants, Год журнала: 2025, Номер 14(10), С. 1472 - 1472

Опубликована: Май 14, 2025

Precursor messenger RNA (pre-mRNA) splicing is a critical post-transcriptional regulatory mechanism in gene expression. The precise of pre-mRNAs essential for plant development and responding to genetic environmental signals. In sexual reproduction, expression regulation relies on the accurate processing pre-mRNAs, which fundamental coordinating developmental programs. alternation generations plants involves two key phases: gametophyte development, produces gametes, fertilization, leads formation diploid sporophyte. Gametophyte embryo represent processes reproduction. This review focuses summarizing analyzing current evidence regarding role pre-mRNA with an emphasis its involvement development. Future challenges understanding reproduction are also discussed, particularly modulating factor levels activities identifying target mRNAs non-coding RNAs regulated by these factors. provides crucial insights into molecular mechanisms reproductive offers theoretical basis improving fertility adaptability via regulation.

Язык: Английский

Процитировано

0
Non‐coding RNAs in plant stress responses: molecular insights and agricultural applications DOI Creative Commons
Xiaotong Chen, Zhaohui Chen, Ryan J. Watts

и другие.

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Май 23, 2025

Summary Non‐coding RNAs (ncRNAs) have emerged as crucial regulators in plant responses to environmental stress, orchestrating complex networks that finetune gene expression under both abiotic and biotic challenges. To elucidate this intricate ncRNA crosstalk, review comprehensively summarizes recent advances understanding the mechanisms of key regulatory ncRNAs including microRNAs (miRNAs), long non‐coding (lncRNAs), circular (circRNAs), tRNA derived fragments (tRFs) small interfering (siRNAs) mediating adaptations stress conditions. We discuss molecular insights into how these modulate signalling pathways, control hormonal interact through elaborate crosstalk mechanisms. also emphasize emerging biotechnological strategies leverage innate artificial well potential approaches for finetuning levels engineer stress‐resilient crops. Collectively, continued high‐throughput sequencing, functional genomics computational modelling will deepen our network mediated responses, ultimately guiding design robust climate‐resilient

Язык: Английский

Процитировано

0
Crossing a CRISPR/Cas9 transgenic tomato plant with a wild-type plant yields diverse mutations in the F1 progeny DOI Creative Commons
Yasuhiro Ito

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Авг. 7, 2024

Generating CRISPR/Cas9-mediated mutants in tomato ( Solanum lycopersicum L.) involves screening shoots regenerated from cultured cells transformed with a T-DNA harboring sequences encoding Cas9 and single guide RNAs (sgRNAs). Production of transformants can be inconsistent obtaining large numbers may difficult, resulting limited variety mutations. Here, I report method for generating various types mutations one transgenic plant the CRISPR/Cas9 system. In this method, wild-type was crossed T 0 biallelic mutant expressing two sgRNAs targeting RIPENING INHIBITOR RIN ) gene, F 1 seedlings were classified using kanamycin resistance marker on T-DNA. Genotyping locus revealed that kanamycin-sensitive seedlings, which carried no T-DNA, always harbored allele parent. Kanamycin-resistant do carry novel alleles, but not allele, suggesting it mutated during crossing. The included one-base insertions or short deletions at each target site, across sites. This also successfully applied to produce Geranylgeranyl pyrophosphate synthase 2 GGPS2 ). Because crossing rather than transformation, readily scaled up numerous mutations, even species cultivars transformation is inefficient. Therefore, when initial transgene experiments fail induce desired mutation, provides additional opportunities mutants.

Язык: Английский

Процитировано

0