Current views of drought research: experimental methods, adaptation mechanisms and regulatory strategies

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Апрель 4, 2024

Drought stress is one of the most important abiotic stresses which causes many yield losses every year. This paper presents a comprehensive review recent advances in international drought research. First, main types and commonly used methods current experiment were introduced, advantages disadvantages each method evaluated. Second, response plants to was reviewed from aspects morphology, physiology, biochemistry molecular progression. Then, potential improve resistance emerging technologies introduced. Finally, research dilemma future development direction summarized. In summary, this provides insights into different perspectives theoretical reference for scholars engaged about engage

Язык: Английский

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Low frequency ultrasound enhanced the antioxidant activity and isoflavones accumulation of soybean sprouts by inducing oxidant stress

Food Bioscience, Год журнала: 2024, Номер 60, С. 104360 - 104360

Опубликована: Май 16, 2024

Язык: Английский

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Tea polyphenol mediated CsMYB77 regulation of CsPOD44 to promote tea plant (Camellia sinensis) root drought resistance

Horticulture Research, Год журнала: 2025, Номер 12(6)

Опубликована: Фев. 18, 2025

Abstract Drought stress significantly alters the metabolic homeostasis of tea plants; however, few studies have examined role specific metabolites, particularly polyphenols, in drought resistance. This study reveals that polyphenol content drought-tolerant cultivars tends to increase under conditions. Notably, environments characterized by staged and repeated drought, changes are positively correlated with To investigate this further, we irrigated roots exogenous polyphenols before subjecting plants drought. Our findings indicated absorptive experimental group exhibited enhanced development, improved cellular integrity, a significant peroxidase activity. A comprehensive analysis transcriptome metabolome revealed closely associated phenylpropanoid metabolism pathway. CsMYB77 CsPOD44 genes were identified as highly Overexpression experiments Arabidopsis thaliana demonstrated promotes expression pathway genes, thereby enhancing Conversely, antisense oligonucleotide silencing decreased resistance plants. Additional experiments, including yeast one-hybrid assays, luciferase complementation imaging, dual-luciferase electrophoretic mobility shift confirmed regulates CsPOD44. In summary, our indicate differences tolerance among linked metabolism. Specifically, may mediate regulatory network involving CsPOD44, promoting root development. offers new insights into breeding drought-resistant cultivars.

Язык: Английский

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Biochemical characterization and metabolic reprogramming of amino acids in Soybean roots under drought stress

Physiologia Plantarum, Год журнала: 2024, Номер 176(3)

Опубликована: Май 1, 2024

Abstract Amino acids play important roles in stress resistance, plant growth, development, and quality, with roots serving as the primary organs for drought response. We conducted biochemical multi‐omics analyses to investigate metabolic processes of root amino drought‐resistant (HN44) drought‐sensitive (HN65) soybean ( Glycine max ) varieties. Our analysis revealed an increase total acid content both varieties, phenylalanine, proline, methionine accumulating both. Additionally, several exhibited significant decreases HN65 but slight increases HN44. Multi‐omics association identified 13 acid‐related pathways. thoroughly examined changes genes metabolites involved various metabolism/synthesis determined core through correlation networks. The tyrosine, tryptophan pathways glutamic sulfur‐containing were particularly resistance. Some candidate genes, such ProDH P4HA family metabolites, O ‐acetyl‐L‐serine, directly affected up‐ downstream metabolism induce This study provided a basis resistance breeding.

Язык: Английский

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Arbuscular mycorrhizal fungi enhance drought resistance and alter microbial communities in maize rhizosphere soil

Environmental Technology & Innovation, Год журнала: 2024, Номер 37, С. 103947 - 103947

Опубликована: Дек. 4, 2024

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Bioinformatics Identification and Expression Analysis of Acetyl-CoA Carboxylase Reveal Its Role in Isoflavone Accumulation during Soybean Seed Development

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(18), С. 10221 - 10221

Опубликована: Сен. 23, 2024

Isoflavones belong to the class of flavonoid compounds, which are important secondary metabolites that play a crucial role in plant development and defense. Acetyl-CoA carboxylase (ACCase) is biotin-dependent enzyme catalyzes conversion into Malonyl-CoA plants. It key fatty acid synthesis also production various metabolites. However, information on ACC gene family soybean (Glycine max L. Merr.) genome specific members involved isoflavone biosynthesis still lacking. In this study, we identified 20 genes (GmACCs) from further characterized their evolutionary relationships expression patterns. Phylogenetic analysis showed GmACCs could be divided five groups, structures within same groups were highly conserved, indicating they had similar functions. The randomly distributed across 12 chromosomes, collinearity suggested many originated tandem segmental duplications, with these being under purifying selection. addition, pattern indicated there was functional divergence among different tissues. reached peak levels during early or middle stages seed development. Based transcriptome content data, weighted co-expression network constructed, three candidate (Glyma.06G105900, Glyma.13G363500, Glyma.13G057400) may positively regulate identified. These results provide valuable for characterization application soybean.

Язык: Английский

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Carbohydrate allocation strategies in leaves of dominant desert shrubs in response to precipitation variability

Agricultural and Forest Meteorology, Год журнала: 2025, Номер 362, С. 110386 - 110386

Опубликована: Янв. 13, 2025

Язык: Английский

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Overexpression of the GmPM35 Gene Significantly Enhances Drought Tolerance in Transgenic Arabidopsis and Soybean

Agronomy, Год журнала: 2025, Номер 15(1), С. 192 - 192

Опубликована: Янв. 15, 2025

Drought stress is one of the major adversity stresses affecting soybean (Glycine max [L.] Merr.) yield. Late embryogenesis abundant protein (LEA protein) a large family proteins widely distributed in various types organisms, and this class plays an important role protecting proteins, membrane lipids, lipids inside cell. The GmPM35 gene member LEA_6 subfamily. expression was significantly increased after drought soybeans. A subcellular localization assay confirmed that acts on cell membrane. Against wild-type Arabidopsis thaliana, we found lines overexpressing were more drought-tolerant at germination seedling stages under stress. To further investigate tolerance function soybeans, nine overexpression T3 generation two editing obtained by Agrobacterium-mediated method using strain (JN28) as receptor. Germination rate, root length, chlorophyll (CHL) content, Proline (Pro) malondialdehyde (MDA) superoxide anion (O2−) hydrogen peroxide (H2O2) (NBT, DAB) staining, activities antioxidant enzymes (CAT, SOD, POD), photosynthetic physiological indexes three different strains measured analyzed before Combined with results rehydration experiments biochemical indices, protected dismutase (SOD), catalase (CAT), peroxidase (POD) average 34.28%, 26.12%, 30.01%, respectively, plants compared Under conditions, showed increase 76.81% photosynthesis rate (Pn), 39.8% transpiration (Tr), 126% stomatal conductance (Gs), 47.71% intercellular CO2 concentration (Ci), 26.44% instantaneous water use efficiency (WUEi). improvement these helped to reduce accumulation reactive oxygen species (ROS) plants. In addition, stress, MDA content reduced 18.8%, Pro 60.14% gene, changes indicated had stronger osmoregulatory capacities response summary, experiment demonstrated can better tolerate maintain normal functions.

Язык: Английский

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Ultrasound-assisted metabolite detection in different extraction processes of Bletilla striata and bitter metabolite detection

Ultrasonics Sonochemistry, Год журнала: 2025, Номер 114, С. 107266 - 107266

Опубликована: Фев. 10, 2025

Bletilla striata, a medicinal orchidaceous plant, is recognized for its significant pharmacological value. However, the lack of comparative metabolomic data across different extraction methods analyzing bioactive components has significantly undervalued application potential B. striata in traditional Chinese medicine market. Using six ultrasound-assisted and UPLC-MS/MS, this study identified 1,945 metabolites extracts. The dominant categories were lipids (51.35%), flavonoids (18.00%), phenolic acids (12.51%). KEGG analysis revealed alterations isoflavonoids biosynthesis pathways. Thirteen bitter metabolites, including cinnamic acid, tubers, underscoring their applications, such as anti-inflammatory, antioxidant antibacterial activities. Optimizing can better preserve extracts, thereby enhancing applications food pharmaceutical industries.

Язык: Английский

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The transcription factor CsPAT1 from tea plant (Camellia sinensis) is involved in drought tolerance by modulating phenylpropanoid biosynthesis

Journal of Plant Physiology, Год журнала: 2025, Номер unknown, С. 154474 - 154474

Опубликована: Март 1, 2025

Язык: Английский

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