Genome and transcriptome-based characterization of cytochrome P450 (CYP) monooxygenase superfamily gene and the key role of ZjCYP98A9a-89 in flavonoid biosynthesis during fruit development of jujube (Ziziphus jujuba mill.)

Food Bioscience, Год журнала: 2025, Номер 64, С. 105855 - 105855

Опубликована: Янв. 7, 2025

Язык: Английский

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TMT-label comparative proteomics reveals the vernalization mechanism in Wucai (Brassica campestris L.)

Journal of Proteomics, Год журнала: 2025, Номер 314, С. 105398 - 105398

Опубликована: Фев. 6, 2025

Язык: Английский

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The key role of cytochrome P450s in the biosynthesis of plant derived natural products

Plant Physiology and Biochemistry, Год журнала: 2025, Номер 222, С. 109695 - 109695

Опубликована: Фев. 25, 2025

Язык: Английский

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Extraction and purification of total flavonoids from Zanthoxylum planispinum Var. Dintanensis leaves and effect of altitude on total flavonoids content

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 27, 2025

Язык: Английский

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Effects of planting patterns on physicochemical properties, metabolites and microbial community structure of rhizosphere soil in perennial cultivated grassland

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 8, 2025

Establishing perennial cultivated grasslands on the Qinghai-Tibet Plateau helps address seasonal imbalance of forage resources and supports restoration degraded grasslands. The most common planting patterns-monocropping mixed cropping-are well-studied in terms vegetation structure, productivity, soil nutrients. Despite their significance, influence prolonged practices underground microbial communities metabolites has often been neglected. In this study, two characteristic plants, Festuca sinensis 'Qinghai' Poa pratensis 'Qinghai', from area around Qinghai Lake were selected as experimental subjects by employing 16 S ITS sequencing methods conjunction with non-targeted metabolomics analysis. effects patterns (monocropping cropping) rhizosphere characteristics, community structure examined. results showed that compared monocropping, cropping significantly increased contents nutrients key metabolites. addition, it had a greater impact fungal diversity than bacterial diversity, particularly β-diversity. While α-diversity dominant phyla remained stable, fungi more responsive to changes properties These show new niche differentiation between different species grassland stimulates secretion trehalose valine, which further affects enhances ecological functions findings will guide selection strategies improve local sustainable productivity.

Язык: Английский

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Phosphorus Alleviates Cadmium Damage by Reducing Cadmium Accumulation and Enhancing Antioxidant Enzymes at the Vegetative Phase in Soybean

Agronomy, Год журнала: 2025, Номер 15(3), С. 637 - 637

Опубликована: Март 3, 2025

Cadmium (Cd) is a toxic, non-essential heavy metal, with significant stress to plants such as soybean (Glycine max). High Cd concentration in the soil inhibits various stages of growth, including seed germination, vegetative and reproduction stage. Phosphate, vital macronutrient, has been shown alleviate Cd-induced stress; however, molecular mechanisms remain poorly understood. This study aimed explore interactive effects phosphate on soybeans at physiological, transcriptomic, metabolic levels using multi-omics approach. Experiments were conducted where treated different concentrations phosphate. The results indicated that significantly reduced plant height, photosynthetic rate, transpiration while phosphorus application mitigated these effects, reducing absorption both roots shoots. Furthermore, antioxidant enzyme activities (superoxide dismutase, catalase, peroxidase) enhanced by under stress, which scavenged reactive oxygen species (ROS) generated cadmium, thereby protecting cells from oxidative damage. Transcriptome metabolome analyses revealed substantial changes gene expression metabolite profiles response treatments. Notably, treatment induced up-regulation genes involved response, root development, metal transport, altering pathways related phenolic acids, flavonoids, lipids. research provided new insights into mechanism activity enzymes, improving plant’s defense capacity toxic cadmium soybeans, offering potential strategies for enhancing crop resilience against contamination.

Язык: Английский

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Gaseous exchange-dependent in vitro culture extensively alters plant growth and metabolic landscape revealed by comprehensive metabolomics-based approach

Plant Physiology and Biochemistry, Год журнала: 2025, Номер 222, С. 109765 - 109765

Опубликована: Март 7, 2025

Язык: Английский

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Integrated Transcriptomic and Metabolomic Analysis Reveals Tissue-Specific Flavonoid Biosynthesis and MYB-Mediated Regulation of UGT71A1 in Panax quinquefolius

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2669 - 2669

Опубликована: Март 16, 2025

Panax quinquefolius is a globally valued medicinal plant rich in bioactive flavonoids, yet the molecular mechanisms underlying their biosynthesis remain poorly understood. In this study, we integrated transcriptomic and metabolomic analyses to investigate tissue-specific flavonoid accumulation regulatory networks roots, leaves, flowers. Metabolomic profiling identified 141 metabolites, with flavones, flavonols, C-glycosylflavones predominantly enriched aerial tissues (leaves flowers), while specific glycosides like tricin 7-O-acetylglucoside showed root-specific accumulation. Transcriptome sequencing revealed 15,551–18,946 DEGs across tissues, reliability of data was validated by qRT-PCR. KEGG GO annotation suggested that these may play crucial role metabolism secondary metabolites. From DEGs, UGTs MYB TFs were subjected correlation analysis. Functional validation through vitro enzymatic assays confirmed PqUGT71A1 catalyzes apigenin naringenin glycosylation at 7-OH position. Additionally, subcellular localization yeast one-hybrid demonstrated PqMYB7 PqMYB13 interact promoter activate its expression.. This study unveils spatial dynamics P. establishes MYB-UGT axis, providing critical insights for metabolic engineering compound optimization plants.

Язык: Английский

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The genus Fraxinus L. (Oleaceae): A review of botany, traditional and modern applications, phytochemistry, and bioactivity

Phytochemistry, Год журнала: 2024, Номер 232, С. 114371 - 114371

Опубликована: Дек. 20, 2024

Язык: Английский

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Integrated metabolomic and transcriptomic analysis of polysaccharides and flavonoids in different tissues of Houttuynia cordata

Scientia Horticulturae, Год журнала: 2024, Номер 338, С. 113729 - 113729

Опубликована: Окт. 17, 2024

Язык: Английский

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