Physiological and molecular profiling unveils oat (Avena sativa L.) defense mechanisms against powdery mildew
Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Май 8, 2025
Oat
powdery
mildew,
caused
by
Blumeria
graminis
f.
sp.
avenae
(Bga),
poses
a
serious
threat
to
oat
production,
yet
the
underlying
resistance
mechanisms
remain
largely
unclear.
In
this
study,
we
investigated
early-stage
defense
responses
in
resistant
(BY642)
and
susceptible
(BY119)
varieties
following
Bga
inoculation
using
cytological
observations,
physiological
hormonal
measurements,
transcriptomic
analysis.
Microscopy
revealed
that
penetrates
tissues
directly
through
epidermal
cells
rather
than
stomata.
BY642
exhibited
rapid
robust
characterized
reactive
oxygen
species
(ROS)
accumulation
hypersensitive
response,
tightly
regulated
an
efficient
antioxidant
system
prevent
cellular
damage.
Hormone
profiling
indicated
salicylic
acid
(SA)-dominated
signaling
pathway
BY642,
accompanied
suppression
of
jasmonic
(JA)
responses.
Transcriptome
showed
early
activation
photosynthesis-related
pathways,
metabolic
reprogramming,
immune-related
networks,
including
MAPK
cascades
WRKY
transcription
factors.
Hub
genes
such
as
AsGSTU6
AsWRKY50
were
identified
key
contributors
resistance.
These
findings
suggest
employs
coordinated
strategy
integrating
ROS
dynamics,
SA
signaling,
transcriptional
regulation,
providing
novel
insights
into
molecular
basis
mildew
potential
targets
for
breeding.
Язык: Английский
+RNA Viruses Enhance Plant Drought Tolerance Through Modulation of Phospholipase Dα1 (PLDα1)‐Derived Phosphatidic Acid (PA)
Plant Cell & Environment,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 21, 2025
ABSTRACT
Numerous
studies
suggest
that
virus
infections
can
improve
drought
resistance
in
host
plants,
but
the
underlying
mechanisms
remain
unclear.
In
this
study,
we
used
Turnip
mosaic
(TuMV),
Potato
Y
(PVY),
Tomato
bushy
stunt
(TBSV)
and
Nicotiana
benthamiana
as
model
systems
to
investigate
these
mechanisms.
Our
findings
reveal
abscisic
acid
(ABA)
signalling
pathway
is
strongly
durably
induced
by
+RNA
infection
essential
for
virus‐induced
tolerance.
Notably,
although
ABA
content
increased
following
infection,
elevation
was
not
necessary
downstream
or
Instead,
tolerance
relies
on
N.
phospholipase
Dα1
(NbPLDα1)‐derived
phosphatidic
(PA).
Knockout
of
NbPLDα1
disruption
interaction
between
viral
proteins
impaired
ability
viruses
activate
enhance
The
increase
levels
appears
result
from
feedback
regulation
PA‐activated
signalling.
Overall,
our
results
plant
modulating
NbPLDα1‐derived
PA
rather
than
promoting
production.
Язык: Английский
The roles of movement and coat proteins in the transport of tobamoviruses between plant cells
Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 17, 2025
Tobamovirus
is
a
large
group
of
positive-sense,
single-stranded
RNA
viruses
that
cause
diseases
in
broad
range
plant
species,
including
many
agronomically
important
crops.
The
number
known
species
has
been
on
the
rise
recent
years,
and
currently,
this
genus
includes
47
viruses.
Tobamoviruses
are
transmitted
mainly
by
mechanical
contact,
such
as
physical
touching
hands
or
agricultural
tools;
some
also
seeds,
through
pollinator
insects.
tobamoviral
genome
encodes
proteins
have
evolved
to
fulfill
main
conceptual
task
viral
infection
cycle
-
spread
invading
virus
throughout
host
cells,
tissues,
organs.
Here,
we
discuss
aspect
tobamoviruses,
focusing
advances
our
understanding
local,
i.e.,
cell-to-cell,
systemic,
organ-to-organ,
movement,
determinants
these
processes.
Specifically,
spotlight
two
proteins—the
movement
protein
(MP)
coat
(CP),
which
directly
involved
local
systemic
tobamoviruses—with
respect
their
phylogeny,
activities
during
interactions
with
process.
Язык: Английский