Integrative Omics reveals genetic basis and TaMYB7-A1's function in wheat WUE and drought resilience DOI Creative Commons
Jun Xiao, Yuxin Zhou, H. M. Wang

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 13, 2024

Improving water use efficiency (WUE) and drought resistance in wheat is critical for ensuring global food security under changing climate conditions. Here, we integrated multi-omic data, including population-scale phenotyping, transcriptomics, genomics, to dissect the genetic molecular mechanisms underlying WUE resilience wheat. Genome-wide association studies (GWAS) revealed 8,135 SNPs associated with WUE-related traits, identifying 258 conditional non-conditional QTLs, many of which co-localized known drought-resistance genes. Pan-transcriptome analysis uncovered tissue-specific expression patterns, core unique gene functions, dynamic sub-genomic biases response drought. eQTL mapping pinpointed 146,966 regulatory loci, condition-specific hotspots enriched genes involved regulation, osmoregulation, photosynthesis. Integration Weighted co-expression network (WGCNA), Summary-data-based Mendelian Randomization (SMR) GWAS, eQTLs identified 207 candidate causal as key regulators traits wheat, such TaMYB7-A1. Functional analyses found that TaMYB7-A1 enhances tolerance by promoting root growth, reducing oxidative stress, improving osmotic enabling better access survival stress. It also increases photosynthesis WUE, boosting yield without compromising performance well-watered conditions, making it ideal target breeding. Our findings provide a comprehensive omic framework understanding architecture resistance, offering valuable targets breeding resilient varieties.

Язык: Английский

Regulatory networks of bZIPs in drought, salt and cold stress response and signaling DOI
Yanli Yang, Yi Xu, Baozhen Feng

и другие.

Plant Science, Год журнала: 2025, Номер unknown, С. 112399 - 112399

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

2
The transcription factor TaWHY2-6A acts as a positive regulator in response to drought tolerance in transgenic plants DOI
Yang Yu,

Conglei Wang,

Xiao Zhang

и другие.

Biochemical and Biophysical Research Communications, Год журнала: 2025, Номер unknown, С. 151580 - 151580

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Overexpression of the Transcription Factor GmbZIP60 Increases Salt and Drought Tolerance in Soybean (Glycine max) DOI Open Access
Mengnan Chai, Fan Yang,

Shuping Cai

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3455 - 3455

Опубликована: Апрель 7, 2025

The regulation of downstream responsive genes by transcription factors (TFs) is a critical step in the stress response system plants. While bZIP are known to play important roles reactions, their functional characterization soybeans remains limited. Here, we identified soybean gene, GmbZIP60, which encodes protein containing typical domain with basic region and leucine zipper region. Subcellular localization studies confirmed that GmbZIP60 localized nucleus. Expression analysis demonstrated induced salt stress, drought various plant hormone treatments, including abscisic acid (ABA), ethylene (ETH), methyl jasmonate (MeJA). Overexpressing (OE-GmbZIP60) transgenic rice enhanced tolerance both stresses. Quantitative real-time polymerase chain reaction (qRT-PCR) indicated expression levels abiotic stress-responsive were significantly higher plants than wild-type (WT) under conditions. Chromatin immunoprecipitation-qPCR (ChIP-qPCR) further directly binds promoters stress-related ABA, ETH, JA, salicylic (SA). Overall, these findings revealed as positive regulator tolerance.

Язык: Английский

Процитировано

0
GmTRAB1, a Basic Leucine Zipper Transcription Factor, Positively Regulates Drought Tolerance in Soybean (Glycine max. L) DOI Creative Commons
Hui Li, Qiuyu Zhang, Ping Xu

и другие.

Plants, Год журнала: 2024, Номер 13(21), С. 3104 - 3104

Опубликована: Ноя. 4, 2024

The basic leucine zipper (bZIP) transcription factors play crucial roles in plant resistance to environmental challenges, but the biological functions of soybean bZIP members are still unclear. In this study, a drought-related gene, GmTRAB1, was analyzed. transcript GmTRAB1 upregulated under drought, ABA, and oxidative stresses. Overexpression improved osmotic stress tolerance transgenic Arabidopsis hairy roots associated with increased proline content activity antioxidant enzymes reduced accumulations malonaldehyde reactive oxide species. However, RNA interference silencing drought sensitivity. Furthermore, sensitivity plants ABA participated modulating ABA-regulated stomatal closure upon stress. addition, stimulated accumulation drought-, ABA-, antioxidant-related genes respond drought. Collectively, research will contribute understanding molecular mechanisms soybean’s

Язык: Английский

Процитировано

3
Integrative Omics reveals genetic basis and TaMYB7-A1’s function in wheat WUE and drought resilience DOI Creative Commons
Jun Xiao, Yuxin Zhou, Hao Wang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 23, 2024

Abstract Improving water use efficiency (WUE) and drought resistance in wheat is critical for ensuring global food security under changing climate conditions. Here, we integrated multi-omic data, including population-scale phenotyping, transcriptomics, genomics, to dissect the genetic molecular mechanisms underlying WUE resilience wheat. Genome-wide association studies (GWAS) revealed 8,135 SNPs associated with WUE-related traits, identifying 258 conditional non-conditional QTLs, many of which co-localized known drought-resistance genes. Pan-transcriptome analysis uncovered tissue-specific expression patterns, core unique gene functions, dynamic sub-genomic biases response drought. eQTL mapping pinpointed 146,966 regulatory loci, condition-specific hotspots enriched genes involved regulation, osmoregulation, photosynthesis. Integration Weighted co-expression network (WGCNA), Summary-data-based Mendelian Randomization (SMR) GWAS, eQTLs identified 207 candidate causal as key regulators traits wheat, such TaMYB7-A1. Functional analyses found that TaMYB7-A1 enhances tolerance by promoting root growth, reducing oxidative stress, improving osmotic enabling better access survival stress. It also increases photosynthesis WUE, boosting yield without compromising performance well-watered conditions, making it ideal target breeding. Our findings provide a comprehensive omic framework understanding architecture resistance, offering valuable targets breeding resilient varieties.

Язык: Английский

Процитировано

1
Integrative Omics reveals genetic basis and TaMYB7-A1's function in wheat WUE and drought resilience DOI Creative Commons
Jun Xiao, Yuxin Zhou, H. M. Wang

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 13, 2024

Improving water use efficiency (WUE) and drought resistance in wheat is critical for ensuring global food security under changing climate conditions. Here, we integrated multi-omic data, including population-scale phenotyping, transcriptomics, genomics, to dissect the genetic molecular mechanisms underlying WUE resilience wheat. Genome-wide association studies (GWAS) revealed 8,135 SNPs associated with WUE-related traits, identifying 258 conditional non-conditional QTLs, many of which co-localized known drought-resistance genes. Pan-transcriptome analysis uncovered tissue-specific expression patterns, core unique gene functions, dynamic sub-genomic biases response drought. eQTL mapping pinpointed 146,966 regulatory loci, condition-specific hotspots enriched genes involved regulation, osmoregulation, photosynthesis. Integration Weighted co-expression network (WGCNA), Summary-data-based Mendelian Randomization (SMR) GWAS, eQTLs identified 207 candidate causal as key regulators traits wheat, such TaMYB7-A1. Functional analyses found that TaMYB7-A1 enhances tolerance by promoting root growth, reducing oxidative stress, improving osmotic enabling better access survival stress. It also increases photosynthesis WUE, boosting yield without compromising performance well-watered conditions, making it ideal target breeding. Our findings provide a comprehensive omic framework understanding architecture resistance, offering valuable targets breeding resilient varieties.

Язык: Английский

Процитировано

0