Genome-Wide Screening of the MYB Genes in Coptis chinensis and Their Roles in Growth, Development, and Heavy Metal Resistance DOI Open Access
Yang Yang, Jingmao You, Xuebo Hu

и другие.

Genes, Год журнала: 2025, Номер 16(5), С. 476 - 476

Опубликована: Апрель 23, 2025

Background: Coptis chinensis is a traditional medicinal plant rich in bioactive compounds like berberine, known for its antibacterial, anti-inflammatory, and antioxidant properties. This study aims to analyze the MYB transcription factor family C. better understand their roles growth, development, metabolism, stress responses. Methods: We employed bioinformatics conduct genome-wide identification of genes chinensis, followed by analyses physicochemical properties, phylogenetic relationships, gene structures, chromosomal localization, conserved motifs, cis-acting elements, expression patterns. Results were validated using qRT-PCR. Results: A total 129 CcMYB identified across nine chromosomes. Phylogenetic analysis categorized these into 19 subgroups, notably highlighting S6 subgroup, which lacks counterparts Arabidopsis. Comparative genomics revealed segmental duplication among pairs. Transcriptomic indicated that CcMYB21, CcMYB40, CcMYB105, CcMYB116 had high levels stems. Importantly, CcMYB94 significantly increased under cadmium stress, suggesting role regulation. Conclusions: offers comprehensive underscoring significance factors enhancing plant’s value tolerance, particularly against exposure. These insights pave way further exploration specific improve resilience chinensis.

Язык: Английский

The Transcription Factor CcMYB330 Regulates Capsaicinoid Biosynthesis in Pepper Fruits DOI Open Access
Hong Cheng, Mingxian Zhang,

Guohua Fang

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1438 - 1438

Опубликована: Фев. 8, 2025

Pepper is an important vegetable and economic crop, the MYB family one of most numerous transcription factor families in plants, extensively participating various biological processes such as plant growth, development, stress resistance. In this study, CcMYB330 identified a differentially expressed gene pepper fruit, with higher expression levels placenta pericarp at different development stages fruit. Analysis promoter cis-elements revealed that contains not only core elements but also environmental response hormone elements. The silencing could reduce capsaicinoid accumulation while overexpression increase accumulation. Additionally, or overexpressing regulate structural genes involved biosynthesis. addition, through yeast one-hybrid experiments, we interaction between biosynthesis CcPAL. Further evidence from EMSA experiments dual luciferase assays confirmed can bind to cis-element ACCAACAACCAAA CcPAL promoter. These results indicate may synthesis capsaicinoids by modulating pathway, providing new insights into regulatory mechanisms synthesis.

Язык: Английский

Процитировано

1
Molecular mechanisms underlying abiotic stress responses in buckwheat DOI Creative Commons
Artur Piński, Syed Muhammad Muntazir Mehdi, Alexander Betekhtin

и другие.

Plant Science, Год журнала: 2025, Номер unknown, С. 112526 - 112526

Опубликована: Апрель 1, 2025

Plants have endured evolutionary changes for hundreds of years under the impact increasing abiotic and biotic stress due to human activities over past centuries. Scientists been working understand molecular mechanisms plant responses severe environmental stress, as plants complex arrangements respond adapt including drought, cold, heat stress. Buckwheat (Fagopyrum spp.) is a resilient pseudocereal known its nutritional value adaptability various conditions, making it an essential crop in sustainable agriculture. It particularly noted gluten-free nature high-quality protein content, which benefit those with gluten sensitivities. However, recent studies revealed that buckwheat cultivation faces significant challenges from stressors such salinity, temperature extremes, heavy metal toxicity, can adversely affect growth yield. We acknowledged key genes factors regulating tolerance response stresses. compiled new data about diverse by different Fagopyrum species manage encompassing physiological, biochemical, adaptations. As global food production demands rise, effective management strategies these are increasingly critical optimising ensuring security changing climate.

Язык: Английский

Процитировано

0
Identification of R2R3-MYB Transcription Factor Family Based on Amaranthus tricolor Genome and AtrMYB72 Promoting Betalain Biosynthesis by Directly Activating AtrCYP76AD1 Expression DOI Creative Commons

Yahui Xue,

Kexuan Li, Wenli Feng

и другие.

Plants, Год журнала: 2025, Номер 14(3), С. 324 - 324

Опубликована: Янв. 22, 2025

MYB (myeloblastosis) is one of the most abundant transcription factors in plants which regulates various biological processes. The molecular characteristics and function R2R3-MYB amaranth remain unclear. In this study, 73 members were identified from genome database we further analyzed their chromosome position, conserved motifs, physiological biochemical features, collinearity relationships, gene structure, phylogeny cis-acting element. Based on phylogenetic expression pattern analysis, 14 candidate genes might be involved betalain synthesis. Amongst genes, level AtrMYB72 was higher ‘Suxian No.1’ than No.2’, also red section green same leaf Amaranthus. overexpression vector pCambia1301-AtrMYB72-GUS VIGS (virus-induced silencing) pTRV2- transferred into leaves via an Agrobacterium-mediated method. results showed that could promote A yeast one-hybrid assay dual luciferase reporter demonstrated bind to AtrCYP76AD1 promoter These indicated promoted biosynthesis by activating transcription. Our provide new insights amaranth.

Язык: Английский

Процитировано

0
The Mutations in RcMYB114 Affect Anthocyanin Glycoside Accumulation in Rose DOI Creative Commons
Maofu Li, Yuan Yang, Hua Wang

и другие.

Biology, Год журнала: 2025, Номер 14(3), С. 258 - 258

Опубликована: Март 4, 2025

In plants, the R2R3-MYB transcription factors are one of largest MYB gene families. These very important for regulating plant growth and development. RcMYB114, RcbHLH, RcWD40 promote anthocyanin accumulation by forming MBW (MYB-bHLH-WD40) complex determine rose flower’s color. RcMYB114 genomic sequences differ between red petal white varieties. Two non-synonymous substitutions were found in open reading frame. It leads to a change amino acids. Here, content showed that there was no petals, while petals increased firstly at stage 2, decreased slightly 4, then again 5. The spatiotemporal expression pattern analysis not expressed all tissues different flower development stages. varieties, highly followed styles, stems, young leaves, 1 However, has highest level blooming stage. sequence contains 9 SNPs coding region, 7 which synonymous had effect on translation product 2 resulted acid alteration positions 116 195, respectively. named RcMYB114a, RcMYB114b. RcMYB114c mutated into leucine via artificial mutation; it valine position Glycine Arginine 195 RcMYB114a RcMYB114d. RcMYB114b double mutation RcMYB114a. results yeast two-hybrid experiments its missense mutations RcMYB114b, RcMYB114c, RcMYB114d could both interact with RcbHLH form MYB-bHLH-WD40 complex. A transient transformation experiment tobacco confirmed significantly high related structural genes tobacco, together gene, led anthocyanins produced color leaves. interacted regulated anthocyanins. two do affect function itself, but accumulated different. This study should provide clues references further research molecular mechanism underlying determination

Язык: Английский

Процитировано

0
Genome-Wide Screening of the MYB Genes in Coptis chinensis and Their Roles in Growth, Development, and Heavy Metal Resistance DOI Open Access
Yang Yang, Jingmao You, Xuebo Hu

и другие.

Genes, Год журнала: 2025, Номер 16(5), С. 476 - 476

Опубликована: Апрель 23, 2025

Background: Coptis chinensis is a traditional medicinal plant rich in bioactive compounds like berberine, known for its antibacterial, anti-inflammatory, and antioxidant properties. This study aims to analyze the MYB transcription factor family C. better understand their roles growth, development, metabolism, stress responses. Methods: We employed bioinformatics conduct genome-wide identification of genes chinensis, followed by analyses physicochemical properties, phylogenetic relationships, gene structures, chromosomal localization, conserved motifs, cis-acting elements, expression patterns. Results were validated using qRT-PCR. Results: A total 129 CcMYB identified across nine chromosomes. Phylogenetic analysis categorized these into 19 subgroups, notably highlighting S6 subgroup, which lacks counterparts Arabidopsis. Comparative genomics revealed segmental duplication among pairs. Transcriptomic indicated that CcMYB21, CcMYB40, CcMYB105, CcMYB116 had high levels stems. Importantly, CcMYB94 significantly increased under cadmium stress, suggesting role regulation. Conclusions: offers comprehensive underscoring significance factors enhancing plant’s value tolerance, particularly against exposure. These insights pave way further exploration specific improve resilience chinensis.

Язык: Английский

Процитировано

0