Peptide-Based Biomimetic Condensates via Liquid–Liquid Phase Separation as Biomedical Delivery Vehicles

Biomacromolecules, Год журнала: 2024, Номер 25(9), С. 5468 - 5488

Опубликована: Авг. 23, 2024

Biomolecular condensates are dynamic liquid droplets through intracellular liquid-liquid phase separation that function as membraneless organelles, which highly involved in various complex cellular processes and functions. Artificial analogs formed via similar pathways can be integrated with biological complexity advanced functions have received tremendous research interest the field of synthetic biology. The coacervate droplet-based compartments partition concentrate a wide range solutes, regarded attractive candidates for mimicking phase-separation behaviors biophysical features biomolecular condensates. use peptide-based materials phase-separating components has advantages such diversity amino acid residues customized sequence design, allows programming their physicochemical properties resulting compartments. In this Perspective, we highlight recent advancements design construction biomimicry from peptides relevant to protein, specific reference molecular self-assembly separation, biorelated applications, envisage emerging biomedical delivery vehicles.

Язык: Английский

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Gaseous Synergistic Self‐Assembly and Arraying to Develop Bio‐Organic Photocapacitors for Neural Photostimulation

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 22, 2025

Abstract Bioinspired supramolecular architectonics is attracting increasing interest due to their flexible organization and multifunctionality. However, state‐of‐the‐art bioinspired generally take place in solvent‐based circumstance, thus leading achieving precise control over the self‐assembly remains challenging. Moreover, intrinsic difficulty of ordering bio‐organic self‐assemblies into stable large‐scale arrays liquid environment for engineering devices severely restricts extensive applications. Herein, a gaseous strategy proposed with physical vapor deposition (PVD) technology, allowing monomers not only self‐assemble architectures well‐established from approaches but morphologies distinct those delivered cases. Specifically, 9‐fluorenylmethyloxycarbonyl‐phenylalanine‐phenylalanine (Fmoc‐FF) self‐assembles spheres tailored dimensions rather than conventional nanofibers, mechanisms. Arraying spherical can integrate behaviors, endorsing film ability programmable optoelectronic properties, which be employed design P‐N heterojunction‐based bio‐photocapacitors non‐invasive nongenetic neurostimulations. The findings demonstrate that may offer an alternative approach achieve unprecedented superstructures, allow behavior integration, potentially paving avenue developing promoting practical applications architectonics.

Язык: Английский

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Piezoelectric Vitamin‐Based Self‐Assemblies for Energy Generation

Advanced Materials, Год журнала: 2025, Номер unknown

Опубликована: Янв. 21, 2025

Abstract Structural diversity of biomolecules leads to various supramolecular organizations and asymmetric architectures self‐assemblies with significant piezoelectric response. However, the piezoelectricity biomolecular has not been fully explored relationship between structures remains poorly understood, which hinders development biomaterials. Herein, for first time, vitamin‐based power generation is systematically explored. X‐ray diffraction studies revealed that vitamin molecules can self‐assemble into different structures, exhibited tunable coefficients ranging from 3.8 42.8 pC N −1 by density functional theory (DFT) calculations. Notably, B 7 D‐biotin (D‐BIO) superior due low crystal symmetry high polarization arrangements. The D‐BIO assemblies‐based nanogenerator (PENG) produced output voltages ≈0.8 V under a mechanical force 47 N, showing durability after 5400 pressing‐releasing cycles stability at least three months. PENG‐based wearable sensor successfully detected bending motions human limbs. Furthermore, insole converted biomechanical energy stable electrical upon foot movement, illuminating 12 light‐emitting diodes (LEDs). This work fills knowledge gaps in self‐assemblies, providing paradigms realizing high‐performance biomaterials through engineering.

Язык: Английский

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Hierarchical Self‐Assembly of Short Peptides: Nanostructure Formation, Function Tailoring, and Applications

Macromolecular Bioscience, Год журнала: 2025, Номер unknown

Опубликована: Янв. 31, 2025

This article explores the hierarchical self-assembly of short peptides, which refers to structured spatial arrangements these molecules over long distances. phenomenon is commonly found in nature and has important implications for biological structure function. Short peptides are preferred because they have ability spontaneously create various nanostructures. process, known as bottom-up assembly, allows addition functional groups at carboxyl or amine ends peptides. These enable specific functions that extremely valuable fields biotechnology biomedicine. text discusses basic processes involved such characteristics amino acid side chains, categorization according their chemical structure, influence intermolecular forces, dynamic process. In addition, paper uses disciplines biomedicine optoelectronics, including stimulus-responsive hydrogels, tissue engineering, drug delivery. The also suggests rational design principles controlling creating new commercial applications, particularly with offers insights into future discipline.

Язык: Английский

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Fabricating N-Cadherin Mimetic Peptide-Based Diverse Self-Assembled Hydrogels in the Presence of Biologically Relevant Cations

Biomacromolecules, Год журнала: 2025, Номер unknown

Опубликована: Март 18, 2025

N-cadherin, a crucial extracellular matrix protein, is in mediating cellular interactions and promoting cell migration. Herein, we have attempted to create N-cadherin mimetic peptide (NcMP) hydrogel scaffolds by incorporating cations as the external stimulus suitable interface for favorable interactions. Inspired Hofmeister series, selected four biologically significant cations, classified kosmotropes chaotropes, varied their concentrations investigate how increasing ionic strength affects self-assembly of NcMP. Interestingly, incorporation these ions greatly influenced self-assembling propensity Fmoc-HAVDI hydrogel, which resulted diverse structural mechanical properties. Such physical properties led differential responses. Thus, were able access biological single gelator molecule simply changing nature concentration relevant cations. We anticipate that systems hold great potential various biomedical applications.

Язык: Английский

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Hierarchically porous carnosine-Zn microspheres

Matter, Год журнала: 2025, Номер unknown, С. 102108 - 102108

Опубликована: Апрель 1, 2025

Язык: Английский

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Self‐Assembly of Noncanonical Peptides: A New Frontier in Cancer Therapeutics and Beyond

Macromolecular Bioscience, Год журнала: 2025, Номер unknown

Опубликована: Апрель 22, 2025

Abstract In addition to the 20 standard amino acids that form building blocks of proteins, nature employs alternative create specialized “noncanonical peptides.” These unique peptides, found in organisms from bacteria humans, often exhibit unconventional structures and functionalities, playing critical roles modulating cellular processes, particularly as antibiotics. Their potential has attracted significant interest for designing novel functional materials based on noncanonical peptides. This review highlights recent advances generation application peptide assemblies. It begins with a definition including classic examples showcase their distinct useful biological activities. Then applications assemblies developing anticancer therapeutics are discussed, focusing representative studies demonstrate efficacy versatility targeting tumor cells. Beyond oncology, it is explored how have been utilized biomaterials, regenerative medicine, molecular imaging catalysis. Finally, perspectives offered future directions this rapidly evolving field, emphasizing exciting opportunities remaining challenges will drive continued innovation applying peptide‐based

Язык: Английский

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Supramolecular Benzophenone-Based Photoinitiator for Spatially-Resolved Polymerization

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Май 12, 2025

Benzophenone-based materials remain widely used as photoinitiators for ultraviolet light-induced free radical polymerizations. Traditionally, polymerization is spatially controlled using top-down techniques such photomasks, which produce well-defined polymeric films. In contrast, we present an alternative method controlling by employing supramolecular to localize the photoinitiator. This approach uses benzophenone-functionalized dipeptides that are specifically tuned enable gel noodle formation, act structural templates. We show of acrylate monomers around noodles can increase Young's modulus up 2 orders magnitude and mechanically robust structures be handled. The self-assembly also explored viscosity SAXS measurements, providing understanding why only 4BPAcFF successfully forms noodles. Our offers a simple yet effective technique localizing polymerization, enabling fine-tuning mechanical properties fabrication intricate designs hollow-core structures.

Язык: Английский

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Bioinspired Imprinted Nanoprobes for Near-Infrared Fluorescence Recognition and Detection of Biogenic Amines

Biosensors and Bioelectronics, Год журнала: 2025, Номер 286, С. 117636 - 117636

Опубликована: Май 27, 2025

Язык: Английский

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Self‐Assembled Fluorescent Peptide Nanoprobes for Disease Diagnosis

Small Methods, Год журнала: 2025, Номер unknown

Опубликована: Июнь 1, 2025

Abstract Benefiting from the advantages of simple reactions, low energy consumption, and uniform properties, self‐assembly has been widely utilized for preparation nanoprobes. Peptides have chosen as “bricks” self‐assembling nano‐biomaterials due to their easy drug formation, highly variable sequence, good biocompatibility biodegradability. can efficiently self‐assemble through noncovalent interactions, such hydrogen bonding, electrostatic π–π stacking, hydrophobic van der Waals forces. Based on this, many ex situ in strategies are developed. The former self‐assembles into stable nanomaterials beforehand, while latter undergoes at target site response its specific stimulus‐responsive modules. As probe sizes miniaturized nanometer scale, they gain high sensitivity, detection limits, capability detection. Nanoprobes created self‐assembly, usually possess targeted accumulation tissues, prolonged elimination half‐life, multimodal imaging capabilities, making them effective disease diagnosis. This review introduces types self‐assembled fluorescent peptide nanoprobes according source fluorescence properties summarizes progress application diagnosis, cancer, neurodegenerative disease, bacterial infection. In addition, limitations will be discussed, new proposed development advanced peptide‐based nanoprobes, aimed improving potential clinical translation.

Язык: Английский

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