Plumbagin controls fungal postharvest pathogens by affecting metabolism and inducing autophagy
Postharvest Biology and Technology,
Год журнала:
2024,
Номер
212, С. 112904 - 112904
Опубликована: Март 21, 2024
Язык: Английский
Current insights into the biocontrol and biotechnological approaches for postharvest disease management of Botrytis cinerea
Postharvest Biology and Technology,
Год журнала:
2024,
Номер
216, С. 113055 - 113055
Опубликована: Июнь 18, 2024
Язык: Английский
A receptor‐like kinase SlFERL mediates immune responses of tomato to Botrytis cinerea by recognizing BcPG1 and fine‐tuning MAPK signaling
New Phytologist,
Год журнала:
2023,
Номер
240(3), С. 1189 - 1201
Опубликована: Авг. 18, 2023
FERONIA
(FER)
is
a
receptor-like
kinase
showing
versatile
functions
during
plant
growth,
development,
and
responses
to
environmental
stimuli.
However,
its
the
interaction
between
fruit
necrotrophic
fungal
pathogens
are
still
unclear.
Combining
reverse
genetic
approaches,
physiological
assays,
co-immunoprecipitation,
protein
phosphorylation
identification,
site-directed
mutagenesis,
we
reported
tomato
FER
homolog
SlFERL
(Solanum
lycopersicum
Like)
involved
in
immune
Botrytis
cinerea
invasion.
The
results
indicated
that
extracellular
domain
recognized
interacted
with
secreted
virulence
BcPG1
from
B.
cinerea,
further
revealed
triggered
downstream
signaling
by
phosphorylating
SlMAP3K18
at
Thr45,
Ser49,
Ser76,
Ser135.
Moreover,
verified
SlMAP2K2
SlMAP2K4
synergistically
contributed
response
of
which
SlFERL-SlMAP3K18
module
substantially
modulated
level
and/or
activity
SlMAP2K2/SlMAP2K4.
These
findings
reveal
new
pattern-triggered
pathway,
indicating
participates
invasion
via
recognizing
fine-tuning
MAPK
signaling.
Язык: Английский
2-Phenylethanol biocontrol postharvest tomato gray mold and its effect on tomato quality
Scientia Horticulturae,
Год журнала:
2024,
Номер
337, С. 113550 - 113550
Опубликована: Авг. 15, 2024
Язык: Английский
Mitigating Response of SlCSE06 Induced by 2-Ethylfuran to Botrytis cinerea Infection
Plants,
Год журнала:
2025,
Номер
14(4), С. 575 - 575
Опубликована: Фев. 13, 2025
Tomato
(Solanum
lycopersicum
L.)
is
a
major
economic
vegetable
crop
globally,
yet
it
prone
to
gray
mold
disease
caused
by
Botrytis
cinerea
infection
during
cultivation.
Caffeoyl
shikimate
esterase
(CSE)
crucial
component
of
the
lignin
biosynthesis
pathway,
which
significantly
contributes
plant
stress
resistance.
Therefore,
investigating
expression
patterns
SlCSE
after
may
offer
theoretical
foundation
for
breeding
resistant
tomato
varieties.
In
this
study,
11
family
members
were
identified
from
genome
using
bioinformatics
analyses.
Public
transcriptome
databases
and
RT-qPCR
experiments
used
analyze
gene
in
tissues,
responses
infection,
temporal
characteristics
response
2-ethylfuran
treatment
infection.
These
resulted
identification
key
SlCSE06.
Transgenic
lines
that
overexpressed
SlCSE06
constructed
examine
their
resistance
levels
disease.
Many
genes
upregulated
when
fruit
infected
with
ripening
stage.
Furthermore,
24
h
2-ethylfuran,
most
exhibited
increased
compared
control
group,
but
they
lower
at
other
time
points.
Thus,
enhance
responsiveness
SlCSEs.
Based
on
research,
was
as
involved
The
SlCSE06-overexpressing
(OE6)
plants
197.94%
increase
wild
type
(WT).
content
OE6
higher
than
WT,
suggesting
overexpression
enhanced
formation
plants.
At
5
days
post-inoculation
cinerea,
lesion
diameter
decreased
31.88%
relative
whereas
370.90%.
level
upregulated,
showing
17.08-fold
WT.
findings
suggest
enhances
activation
critical
stress,
thereby
promoting
deposition
mitigate
further
cinerea.
Язык: Английский
Temporal proteome profiling of Botrytis cinerea reveals proteins involved in plant invasion and survival
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Апрель 7, 2025
Abstract
Botrytis
cinerea
is
a
necrotrophic
fungal
pathogen
that
poses
significant
threat
to
many
crops.
Understanding
the
proteome
dynamics
of
phytopathogens
during
infection
can
help
combat
plant
diseases.
However,
most
proteomics
studies
in
face
interference
from
abundant
host
proteins.
Here,
we
optimized
solid
media
better
mimics
in-planta
conditions
and
used
it
perform
temporal
protein
.
An
agar
with
20%
tomato
fruit
extract
2%
deproteinised
leaf
was
utilized
for
label-free
quantitative
at
12,
36,
72
120
hpi.
Out
3244
quantified
proteins,
2045
showed
differential
regulation.
Glycosyl
hydrolases,
pectin
esterases,
stress
DDR48,
RhoGEF
essential
transcription
factors
were
found
be
upregulated
early
phase,
highlighting
their
role
virulence.
Meanwhile,
pathways
such
as
macromolecule
synthesis,
purine,
carbohydrate
metabolism
late-growth
phase.
Overall,
study
provides
comprehensive
understanding
infection.
Язык: Английский
Characterization of two SGNH family cell death-inducing proteins from the horticulturally important fungal pathogen Botrytis cinerea based on the optimized prokaryotic expression system
Molecular Horticulture,
Год журнала:
2024,
Номер
4(1)
Опубликована: Март 7, 2024
Abstract
Botrytis
cinerea
is
one
of
the
most
destructive
phytopathogenic
fungi,
causing
significant
losses
to
horticultural
crops.
As
a
necrotrophic
fungus,
B.
obtains
nutrients
by
killing
host
cells.
Secreted
cell
death-inducing
proteins
(CDIPs)
play
crucial
role
in
infection;
however,
only
limited
number
have
been
reported.
For
high-throughput
CDIP
screening,
we
optimized
prokaryotic
expression
system
and
compared
its
efficiency
with
other
commonly
used
protein
systems.
The
showed
superior
effectiveness
was
selected
for
subsequent
screening.
screening
verified
fifty-five
candidate
identified
two
novel
SGNH
family
CDIPs:
BcRAE
BcFAT.
BcFAT
exhibited
high
levels
throughout
infection
process.
Site-directed
mutagenesis
targeting
conserved
Ser
residues
abolished
activity
both
Moreover,
transient
plants
enhanced
plant
resistance
against
without
inducing
death,
independent
their
enzymatic
activities.
Our
results
suggest
high-efficiency
provide
new
targets
further
study
-plant
interactions.
Язык: Английский
2-Heptanol inhibits Botrytis cinerea by accelerating amino acid metabolism and retarding membrane transport
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Июнь 3, 2024
During
the
postharvest
storage
of
tomatoes,
they
are
susceptible
to
infection
by
Botrytis
cinerea
,
leading
significant
economic
losses.
This
study
evaluated
antifungal
potential
2-heptanol
(2-HE),
a
volatile
biogenic
compound,
against
B.
and
explored
underlying
mechanism.
The
results
indicated
that
2-HE
effectively
suppressed
growth
mycelia
both
in
vivo
vitro
stimulated
activities
antioxidative
enzymes,
including
superoxide
dismutase
(SOD),
peroxidase
(POD),
catalase
(CAT)
tomatoes.
Furthermore,
reduced
spore
viability,
compromised
membrane
integrity,
resulted
increased
levels
extracellular
nucleic
acids,
protein
content,
lipid
peroxidation.
Transcriptomic
analysis
revealed
disrupted
transport
system
enhanced
amino
acid
metabolism,
which
led
intracellular
nutrient
depletion
subsequent
cell
death.
Additionally,
treatment
did
not
negatively
impact
appearance
or
quality
In
conclusion,
findings
this
offer
insights
into
use
as
biocontrol
agent
food
agricultural
applications.
Язык: Английский
Transcriptomic and metabolomic analyses provide evidence on the biocontrol mechanism responsible for the inhibition of Botrytis cinerea and Alternaria alternata by Bacillus velezensis in kiwifruit
Food Frontiers,
Год журнала:
2024,
Номер
5(6), С. 2623 - 2640
Опубликована: Авг. 20, 2024
Abstract
Botrytis
cinerea
and
Alternaria
alternata
are
major
pre‐
postharvest
plant
pathogens
of
kiwifruit
responsible
for
significant
economic
losses.
Although
synthetic
fungicides
the
main
source
controlling
these
pathogens,
their
use
can
lead
to
pathogen
resistance,
pollution
environment,
residues
on
agricultural
produce
represent
a
health
risk
humans.
Bacillus
velezensis
is
an
ecofriendly
bacterium
with
biocontrol
potential.
The
objectives
present
study
were
determine
efficacy
B.
against
A.
,
both
which
kiwifruit,
investigate
mechanisms
its
activity
using
transcriptomic
metabolomic
methodology.
Dual
cultures
either
or
versus
single
used
conduct
analyses
provide
insight
into
mechanism
by
inhibits
.
A
total
2499
3248
differentially
expressed
genes
(DEGs)
identified
in
/
velezensis/A.
comparisons,
respectively.
Genes
related
sporulation,
virulence,
hydrolase
downregulated
associated
MAPK
signaling
TCA
cycle
also
downregulated.
Our
provides
new
insights
underlying
inhibition
results
demonstrate
potential
as
agent
two
kiwifruit.
Язык: Английский