Polymer- and lipid-based nanocarriers for ocular drug delivery: Current status and future perspectives

Advanced Drug Delivery Reviews, Год журнала: 2023, Номер 196, С. 114770 - 114770

Опубликована: Март 7, 2023

Ocular diseases seriously affect patients' vision and life quality, with a global morbidity of over 43 million blindness. However, efficient drug delivery to treat ocular diseases, particularly intraocular disorders, remains huge challenge due multiple barriers that significantly the ultimate therapeutic efficacy drugs. Recent advances in nanocarrier technology offer promising opportunity overcome these by providing enhanced penetration, increased retention, improved solubility, reduced toxicity, prolonged release, targeted loaded eyes. This review primarily provides an overview progress contemporary applications nanocarriers, mainly polymer- lipid-based treating various eye highlighting their value achieving delivery. Additionally, covers administration routes, as well prospective future developments challenges field nanocarriers for diseases.

Язык: Английский

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Nanoceria-Mediated Cyclosporin A Delivery for Dry Eye Disease Management through Modulating Immune–Epithelial Crosstalk

ACS Nano, Год журнала: 2024, Номер 18(17), С. 11084 - 11102

Опубликована: Апрель 18, 2024

Dry eye disease (DED) affects a substantial worldwide population with increasing frequency. Current single-targeting DED management is severely hindered by the existence of an oxidative stress–inflammation vicious cycle and complicated intercellular crosstalk within ocular microenvironment. Here, nanozyme-based drop, namely nanoceria loading cyclosporin A (Cs@P/CeO2), developed, which possesses long-term antioxidative anti-inflammatory capacities due to its regenerative activity sustained release (CsA). In vitro studies showed that dual-functional Cs@P/CeO2 not only inhibits cellular reactive oxygen species production, sequentially maintaining mitochondrial integrity, but also downregulates inflammatory processes repolarizes macrophages. Moreover, using flow cytometric single-cell sequencing data, in vivo therapeutic effect was systemically demonstrated, rebalances immune–epithelial communication corneal microenvironment less macrophage polarization, restrained stress, enhanced epithelium regeneration. Collectively, our data proved may provide insights into management.

Язык: Английский

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A “T.E.S.T.” hydrogel bioadhesive assisted by corneal cross-linking for in situ sutureless corneal repair

Bioactive Materials, Год журнала: 2023, Номер 25, С. 333 - 346

Опубликована: Фев. 11, 2023

Corneal transplantation is an effective clinical treatment for corneal diseases, which, however, limited by donor corneas. It of great value to develop bioadhesive patches with functions "Transparency" and "Epithelium & Stroma generation", as well "Suturelessness" "Toughness". To simultaneously meet the "T.E.S.T." requirements, a light-curable hydrogel designed based on methacryloylated gelatin (GelMA), Pluronic F127 diacrylate (F127DA) Aldehyded (AF127) co-assembled bi-functional micelles collagen type I (COL I), combined clinically applied cross-linking (CXL) technology repairing damaged cornea. The patch formed after 5 min ultraviolet irradiation possesses transparent, highly tough, strongly bio-adhesive performance. Multiple makes withstand deformation near 600% exhibit burst pressure larger than 400 mmHg, significantly higher normal intraocular (10-21 mmHg). Besides, slower degradation GelMA-F127DA&AF127 without COL stable stromal beds in vivo, supporting regrowth epithelium stroma. can replace deep defects bio-integrate into tissue rabbit models within 4 weeks, showing potential surgeries keratoconus other diseases combining CXL.

Язык: Английский

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Dopamine‐Based High‐Transparent Hydrogel as Bioadhesive for Sutureless Ocular Tissue Repair

Advanced Functional Materials, Год журнала: 2023, Номер 33(49)

Опубликована: Авг. 4, 2023

Abstract Ocular injuries and their complications represent the most common causes of visual impairment. For ocular surgery, there is an unmet need for highly transparent bioadhesives with superior adhesion, biocompatibility, regenerative properties. Herein, a novel high‐transparent bioadhesive hydrogel composed gelatin methacryloyl (GelMA) dopamine methacrylamide (DMA) developed by in situ oxidative free‐radical polymerization. This overcomes fundamental weakness mussel‐inspired adhesive copolymers clinical practice combining multiple favorable properties, including high light transmission, mechanical strength, biocompatibility. DMA significantly enhances corneal epithelial cells proliferation, migration on GelMA, prevents accumulation reactive oxygen species (ROS) cells. In rabbit models conjunctiva transplantation, able to decrease inflammatory response fibrosis formation induced suture surgical trauma. addition, stromal defect model reveals that Gel/DMA could effectively seal defects, accelerates re‐epithelialization, promotes wound healing. Thus, given advantages bioactivity simple preparation, represents promising strategy suture‐free repair.

Язык: Английский

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A Versatile Hydrogel with Antibacterial and Sequential Drug-Releasing Capability for the Programmable Healing of Infectious Keratitis

ACS Nano, Год журнала: 2023, Номер 17(23), С. 24055 - 24069

Опубликована: Дек. 4, 2023

Hydrogels have attracted tremendous attention as favorable corneal substitutes for treating severe infectious keratitis (IK). However, current hydrogel-based were majorly designed to promote the single stage of regeneration, which falls short in meeting clinical management needs IK including multiple phases wound healing. Herein, we introduce a versatile hybrid hydrogel (SQPV) composed silk fibroin and chitosan, exhibits spatiotemporal properties drug release. The SQPV is fabricated by incorporating verteporfin-loaded poly(lactic-co-glycolic)-polyethylene glycol-o-nitrobenzene micelles into network, formed from methacrylate glycidyl functionalized quaternized chitosan containing polydeoxyribonucleotide. This double network approach results material with exceptional anti-inflammatory, antibacterial, proliferative stimulation tissue remodeling regulation capabilities. Furthermore, showcases mechanical strength transparency akin those native cornea. Extensive vitro vivo studies validate SQPV's ability effectively eliminate residual bacteria, mitigate inflammation, foster regeneration epithelium stroma, prevent scarring, ultimately expedite In summary, SF/CS-based may represent promising substitute comprehensive repair IK.

Язык: Английский

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Photocrosslinked methacrylated natural macromolecular hydrogels for tissue engineering: A review

International Journal of Biological Macromolecules, Год журнала: 2023, Номер 246, С. 125570 - 125570

Опубликована: Июнь 25, 2023

Язык: Английский

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Microneedles for in situ tissue regeneration

Materials Today Bio, Год журнала: 2023, Номер 19, С. 100579 - 100579

Опубликована: Фев. 11, 2023

Tissue injury is a common clinical problem, which may cause great burden on patients' life. It important to develop functional scaffolds promote tissue repair and regeneration. Due their unique composition structure, microneedles have attracted extensive attention in various tissues regeneration, including skin wound, corneal injury, myocardial infarction, endometrial spinal cord et al. Microneedles with micro-needle structure can effectively penetrate the barriers of necrotic or biofilm, therefore improving bioavailability drugs. The use deliver bioactive molecules, mesenchymal stem cells, growth factors

Язык: Английский

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Current Advances in Corneal Stromal Stem Cell Biology and Therapeutic Applications

Cells, Год журнала: 2024, Номер 13(2), С. 163 - 163

Опубликована: Янв. 16, 2024

Corneal stromal stem cells (CSSCs) are of particular interest in regenerative ophthalmology, offering a new therapeutic target for corneal injuries and diseases. This review provides comprehensive examination CSSCs, exploring their anatomy, functions, role maintaining integrity. Molecular markers, wound healing mechanisms, potential applications discussed. Global blindness, especially more resource-limited regions, underscores the need innovative solutions. Challenges posed by defects, emphasizing urgent advanced interventions, The places spotlight on exosome therapy as therapy. CSSC-derived exosomes exhibit significant modulating inflammation, promoting tissue repair, addressing transparency. Additionally, rejuvenation CSSCs through epigenetic reprogramming adds to evolving landscape. imperative clinical trials human studies seamlessly integrate these strategies into practice is emphasized. points towards future where CSSC-based therapies, particularly leveraging exosomes, play central diversifying ophthalmic medicine.

Язык: Английский

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Squishy matters – Corneal mechanobiology in health and disease

Progress in Retinal and Eye Research, Год журнала: 2024, Номер 99, С. 101234 - 101234

Опубликована: Янв. 2, 2024

The cornea, as a dynamic and responsive tissue, constantly interacts with mechanical forces in order to maintain its structural integrity, barrier function, transparency refractive power. Cells within the cornea sense respond various that fundamentally regulate their morphology fate development, homeostasis pathophysiology. Corneal cells also dynamically extracellular matrix (ECM) ensuing cell-ECM crosstalk serves signaling reservoir providing biophysical biochemical cues corneal cells. Here we provide an overview of mechanotransduction pathways then delve into recent advances mechanobiology, focusing on interplay between responses epithelial, stromal, endothelial We identify species-specific differences biomechanics facilitate identification optimal animal models study wound healing, disease, novel therapeutic interventions. Finally, key knowledge gaps opportunities mechanobiology are pressing for research community address especially pertinent domains limbal stem cell deficiency, keratoconus Fuchs' dystrophy. By furthering our understanding can contextualize discoveries regarding diseases well innovative treatments them.

Язык: Английский

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Fostering the unleashing potential of nanocarriers-mediated delivery of ocular therapeutics

International Journal of Pharmaceutics, Год журнала: 2024, Номер 658, С. 124192 - 124192

Опубликована: Май 3, 2024

Ocular delivery is the most challenging aspect in field of pharmaceutical research. The major hurdle for controlled drugs to eye includes physiological static barriers such as complex layers cornea, sclera and retina which restrict drug from permeating into anterior posterior segments eye. Recent years have witnessed inventions conventional nanocarrier shown considerable enhancement delivering small large molecules across dynamic challenges associated with systems include limited contact time inadequate ocular bioavailability resulting solution drainage, tear turnover, dilution or lacrimation. To this end, various bioactive-based nanosized carriers including liposomes, ethosomes, niosomes, dendrimer, nanogel, nanofibers, lenses, nanoprobes, selenium nanobells, nanosponge, polymeric micelles, silver nanoparticles, gold nanoparticles among others been developed circumvent limitations dosage forms. These nanocarriers achieve enhanced permeation retention prolong release tissue due their better adherence. surface charge size (10–1000 nm) are important key factors overcome barriers. Various deliver active therapeutic timolol maleate, ampicillin, natamycin, voriconazole, cyclosporine A, dexamethasone, moxifloxacin, fluconazole treatment diseases. Taken together, a nutshell, book chapter provides comprehensive perspective on numerous facets special focus bioactive nanocarrier-based approaches, difficulties constraints involved fabrication nanocarriers. This also detailed invention applications ophthalmic therapeutics.

Язык: Английский

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