Neural Circuit Revision in Retinal Remodeling, A Pathoconnectomics Approach

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 15, 2024

Abstract The Aii glycinergic amacrine cell (Aii) plays a central role in bridging rod pathways with cone pathways, enabling an increased dynamic range of vision from scotopic to photopic ranges. integrates signals via chemical synapses bipolar cells (RodBCs) onto the arboreal processes ACs, injecting into ON-cone (CBbs) gap junctions Aiis on and waist ACs. CBbs then carry this information ON OFF ganglion classes. In addition, is involved surround inhibition (CBas) through ACs CBas. We have previously shown changes RodBC connectivity as consequence photoreceptor degeneration pathoconnectome early retinal degeneration: RPC1. Here, we evaluated impact determine impacts downstream network neural retina its suitability for integrating therapeutic interventions photoreceptors are lost. Previously, reported that degeneration, prior loss, Rod BCs make pathological Aiis. further characterize altered additional shifts both excitatory drive junctional coupling along discussion broader networks. New findings here demonstrate CBas increasing number BC classes degenerating retina. study, also report Aii, tertiary neuron alters their synaptic contacts indicating rewiring occurs more distant members earlier than was predicted. This processing, presumably acuity, ultimately ability support therapeutics designed restore image-forming vision. Finally, these alterations may be cellular level finding explains one first clinical complaints human patients degenerative disease, inability adapt back forth conditions.

Язык: Английский

---

Altered Functional Responses of the Retina in B6 Albino Tyr^c/c Mice

Investigative Ophthalmology & Visual Science, Год журнала: 2024, Номер 65(10), С. 39 - 39

Опубликована: Авг. 27, 2024

Mammals with albinism present low visual discrimination ability and different proportions of certain retinal cell subtypes. As the spatial resolution retina depends on field sampling by ganglion cells (RGCs) based convergence upstream inputs, it could be affected in thus modify RGC function.

Язык: Английский

---

Cellular Characterization and Interspecies Evolution of the Tree Shrew Retina across Postnatal Lifespan

Research, Год журнала: 2024, Номер 7

Опубликована: Янв. 1, 2024

Tree shrews (TSs) possess a highly developed visual system. Here, we establish an age-related single-cell RNA sequencing atlas of retina cells from 15 TSs, covering 6 major cell classes and 3 glial types. An age effect is observed on the subset composition gene expression pattern. We then verify subtypes identify specific markers in TS including CA10 for bipolar cells, MEGF11 H1 horizontal SLIT2 , RUNX1 FOXP2 SPP1 retinal ganglion subpopulations. The cross-species analysis elucidates type-specific transcriptional programs, different compositions, communications. comparisons also reveal that cones subclasses amacrine exhibit closest relationship with humans macaques. Our results suggests could be used as better disease model to understand age-dependent cellular genetic mechanisms retina, particularly diseases associated cones.

Язык: Английский

---

The long-term outcome of eyes with unexplained visual loss after gas tamponade for macula-on retinal detachment

Retinal Cases & Brief Reports, Год журнала: 2023, Номер 18(4), С. 491 - 495

Опубликована: Март 29, 2023

Purpose: To analyze the long-term outcome of eyes suffering from unexplained visual loss after gas tamponade for primary macula-sparing rhegmatogenous retinal detachment. Methods: Cross-sectional analysis all with macula-on detachment experiencing an vision reabsorption that were treated and followed 2010 to 2019. The investigational work-up included: best-corrected acuity (BCVA), clinical examination, spectral-domain optical coherence tomography, automated computerized perimetry. Results: nine patients analyzed 5.9 ± 2.4 years. Best-corrected improved by 0.54 0.50 logMAR baseline, achieving a final value 1.17 0.52 (∼20/320; P = 0.0115). thicknesses macula, macular ganglion cells, nerve fiber layers unchanged compared rate ellipsoid zone defects (22.2%). proportion microcystoid edema significantly decreased 44.4% ( 0.0294). Perimetry mean deviation increased baseline −18.06 2.72 −17.23 2.29 dB 0.0390), pattern SD 0.1289). In general, reduction scotomata relative depth was evident in eyes. Conclusion: Eyes reabsorption, despite disclosing overall structural morphology, showed moderate, but significant, perimetric improvement long-term.

Язык: Английский

---

Contribution of membrane-associated oscillators to biological timing at different timescales

Опубликована: Июнь 26, 2023

Environmental rhythms such as the daily light-dark cycle selected for endogenous clocks. These clocks predict regular environmental changes and provide basis well-timed adaptive homeostasis in physiology behavior of organisms. Endogenous are oscillators that based on positive feedforward negative feedback loops. They generate stable even under constant conditions. Since weak interactions between allow autonomous synchronization, coupling/synchronization provides self-organized physiological timing. Amongst most thoroughly researched circadian clock neurons mammals insects. comprise nuclear clockworks transcriptional/translational loops (TTFL) ~24 h gene expression entrained to day-night cycle. It is generally assumed this TTFL clockwork drives all oscillations within cells, being any rhythm Instead current gene-based hierarchical model we here a systems view We suggest coupled system posttranslational loop (PTFL) oscillators/clocks run at multiple timescales governs adaptive, dynamic behavior. focus mammalian insect timescales. neuronal plasma membrane-associated signalosomes constitute specific PTFL localized but interlinked membrane potential intracellular messengers with frequencies. In each neuron multiscale form temporally structured oscillatory network common complex frequency-band comprising superimposed oscillations. Coupling oscillator/clock next level complexity an network. This systemic molecular cellular suggested cycles through homeostatic setpoints characteristic hallmark. propose mechanisms plasticity maintain stability these setpoints, whereas Hebbian enables switching via coupling factors, like biogenic amines and/or neuropeptides. reprogram new frequency, setpoint. Our novel hypothesis up experimental challenge.

Язык: Английский

---

Translational roadmap for regenerative therapies of eye disease

Med, Год журнала: 2023, Номер 4(9), С. 583 - 590

Опубликована: Сен. 1, 2023

Язык: Английский

---