Structural Characterization of a Novel Lactuca Sativa Polysaccharide and its Adjuvant Hypoglycemic Effect in Hfd/Stz-Induced Obese C57 Bl/6 J Mice

Опубликована: Янв. 1, 2025

A novel natural polysaccharide (LSP) was isolated and purified from Lactuca sativa L. The structure of LSP characterized using various techniques, including FT-IR, IC, Congo red, XRD, DSC, SEM, AFM, NMR. Furthermore, its in vivo hypoglycemic activity as an adjuvant studied a diabetic mouse model induced by HFD/STZ. findings revealed that possesses molecular weight 1.3 × 10⁶ kDa, with primary structural elements identified →4)-α-D-Glcp-(1→, →4,6)-α-Glcp-(1→, α-Araf-(1→, →6)-β-D-Galp-(1→, →3,4)-β-Xylp-(1→, →4)-β-D-GalpA-(1→. contains Ara, Gal, Glc, Xyl GalA molar ratio 0.44:1:2.07:2.11:0.77. is semi-crystalline polymer lacking triple helix exhibiting stable thermal properties below 200°C. Studies on demonstrated the conjunction metformin significantly improved effects, decreased body weight, boosted antioxidant capacity, enhanced lipid profiles, insulin sensitivity, hepatic glycolysis-related enzyme activities mice compared to alone. This research presents conceptual rationale for exploring function polysaccharides, highlighting their potential adjunctive role control diabetes mellitus.

Язык: Английский

Structural characterization of a novel Lactuca sativa L. polysaccharide and its adjuvant hypoglycemic effect in HFD/STZ-induced obese C57 BL/6 J mice

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106458 - 106458

Опубликована: Апрель 1, 2025

Язык: Английский