Tumor‐associated exosomes in cancer progression and therapeutic targets
MedComm,
Год журнала:
2024,
Номер
5(9)
Опубликована: Сен. 1, 2024
Exosomes
are
small
membrane
vesicles
that
released
by
cells
into
the
extracellular
environment.
Tumor-associated
exosomes
(TAEs)
play
a
significant
role
in
cancer
progression
mediating
intercellular
communication
and
contributing
to
various
hallmarks
of
cancer.
These
carry
cargo
proteins,
lipids,
nucleic
acids,
other
biomolecules
can
be
transferred
recipient
cells,
modifying
their
behavior
promoting
tumor
growth,
angiogenesis,
immune
modulation,
drug
resistance.
Several
potential
therapeutic
targets
within
TAEs
have
been
identified,
including
oncogenic
miRNAs,
tumor-associated
antigens,
checkpoint
resistance
tissue
factor.
In
this
review,
we
will
systematically
summarize
biogenesis,
composition,
function
highlight
targets.
Considering
complexity
exosome-mediated
signaling
pleiotropic
effects
exosome
cargoes
has
challenge
developing
effective
strategies.
Further
research
is
needed
fully
understand
develop
therapies
target
them.
particular,
development
strategies
block
release,
cargo,
inhibit
uptake,
modulate
content
could
provide
novel
approaches
treatment.
Язык: Английский
Identification of a Plasma Exosomal lncRNA‐ and circRNA‐Based ceRNA Regulatory Network in Patients With Lung Adenocarcinoma
The Clinical Respiratory Journal,
Год журнала:
2024,
Номер
18(10)
Опубликована: Окт. 1, 2024
ABSTRACT
Background
Exosomes
have
been
established
to
be
enriched
with
various
long
noncoding
RNAs
(lncRNAs)
and
circular
(circRNAs)
that
exert
biological
effects.
However,
the
lncRNA‐
circRNA‐mediated
coexpression
competing
endogenous
RNA
(ceRNA)
regulatory
network
in
exosomes
derived
from
plasma
of
patients
lung
adenocarcinoma
(LUAD)
remains
elusive.
Methods
Results
This
study
enrolled
nine
three
healthy
individuals,
differential
expression
messenger
(mRNAs),
lncRNAs,
circRNAs
was
detected
using
microarray
analysis,
while
microRNAs
(miRNAs)
were
through
sequencing.
Additionally,
bioinformatics
algorithms
applied
evaluate
lncRNA–miRNA–mRNAs/circRNA–miRNA–mRNA
network.
Differentially
expressed
cicRNAs
identified
via
quantitative
reverse
transcription
polymerase
chain
reaction
(RT‐qPCR).
A
total
1016
1396
circRNAs,
45
miRNAs,
699
mRNAs
differentially
LUAD
compared
controls.
Among
them,
881
lncRNAs
upregulated
135
downregulated,
916
480
miRNAs
none
591
108
downregulated
(
p
≤
0.05,
fold
change
≥
2).
Gene
Ontology
(GO)
analysis
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
pathway
revealed
functions
RNAs.
Meanwhile,
networks
displayed
relationship
between
dysregulated
Finally,
RT‐qPCR
validated
circ‐0033861,
circ‐0043273,
circ‐0011959
exosome
controls
=
0.0327,
0.0002,
0.0437,
respectively).
Conclusion
proposed
a
newly
discovered
ncRNA–miRNA–mRNA/circRNA–miRNA–mRNA
ceRNA
circulating
up‐regulated
LUAD,
offering
valuable
insights
for
exploring
potential
function
exosomal
identifying
biomarkers
LUAD.
Язык: Английский
Profiling Plasma Extracellular Vesicle Metabotypes and miRNAs: An Unobserved Clue for Predicting Relapse in Patients with Early-Stage NSCLC
Cancers,
Год журнала:
2024,
Номер
16(22), С. 3729 - 3729
Опубликована: Ноя. 5, 2024
Lung
cancer,
the
second
most
prevalent
cancer
globally,
poses
significant
challenges
in
early
detection
and
prognostic
assessment.
Despite
advancements
targeted
therapies
immunotherapy,
timely
identification
of
relapse
remains
elusive.
Blood-based
liquid
biopsy
biomarkers,
including
circulating
tumor
cells
(CTCs),
cell-free
DNA
(cfDNA),
(ctDNA),
circulating-free
RNAs
(cfRNAs),
extracellular
vesicles
(EVs)/exosomes,
offer
promise
for
non-invasive
monitoring.
Язык: Английский
MicroRNAs as Potential Graft Rejection or Tolerance Biomarkers and Their Dilemma in Clinical Routines Behaving like Devilish, Angelic, or Frightening Elements
Biomedicines,
Год журнала:
2024,
Номер
12(1), С. 116 - 116
Опубликована: Янв. 5, 2024
Allograft
rejection
is
a
widespread
complication
in
allograft
recipients
with
chronic
kidney
disease.
Undertreatment
of
subclinical
and
clinical
later
post-transplant
problems
are
caused
by
an
imperfect
understanding
the
mechanisms
at
play
lack
adequate
diagnostic
tools.
Many
different
biomarkers
have
been
analyzed
proposed
to
detect
monitor
these
crucial
events
transplant
outcomes.
In
this
sense,
microRNAs
may
help
diagnose
or
tolerance
indicate
appropriate
treatment,
especially
patients
rejection.
As
key
epigenetic
regulators
physiological
homeostasis,
therapeutic
potential
However,
more
evidence
validation
indispensable
before
ready
for
prime
time.
Язык: Английский
Downregulation of hsa_circTLK1 represses non-small cell lung cancer progression by regulating miR-876-3p/SRSF7 axis
Heliyon,
Год журнала:
2024,
Номер
10(11), С. e31972 - e31972
Опубликована: Май 25, 2024
Backgrounds:
This
study
clarified
the
expression
of
cicrTLK1
in
non-small
cell
lung
cancer
(NSCLC)
and
explored
its
role
growth,
metastasis
immune
escape,
providing
a
potential
molecular
target
theoretical
basis
for
NSCLC
immunotherapy.MethodsThe
levels
circTLK1,
miR-876-3p
SRSF7
were
determined
by
RT-qPCR
assay.
The
localization
circTLK1
cells
was
FISH
EdU
plate
clone
formation
assay
applied
to
explore
proliferation.
Wound
healing
test
Transwell
measure
migration
invasion
ability.
Cell
apoptosis
rate
detected
FCM
Western
blotting
adopted
protein
SRSF7.
Dual-luciferase
reporter
gene
assess
interaction
between
ability
cirTLK1
regulate
tumor
vivo
examined
transplantation
experiments
nude
mice.ResultsThe
relative
increased
lines.
Knockdown
prohibited
proliferation,
migration,
invasion,
promoted
rate,
but
inhibitor
reversed
effects
knockdown.
In
addition,
silencing
overtly
restrained
growth
transplanted
tumors
vivo,
inhibited
escape.
interacted
with
miR-876-3p,
concluded
be
miR-876-3p.ConclusionIn
this
study,
we
researched
inhibitory
effect
circTLK1knockdown
on
progression
further
elucidated
regulatory
mechanism
circTLK1/miR876-3p/SRSF71
axis.
Язык: Английский
Integrative Biomarker Panel for Improved Lung Cancer Diagnosis Using Plasma microRNAs and Sputum Bacterial DNA
Current Oncology,
Год журнала:
2024,
Номер
31(10), С. 5949 - 5959
Опубликована: Окт. 2, 2024
This
study
aimed
to
evaluate
if
integrating
diverse
molecular
biomarkers
in
plasma
and
sputum
could
improve
the
diagnosis
of
lung
cancer.
The
analyzed
miRNAs
bacterial
DNA
from
58
cancer
patients
62
cancer-free
smokers
using
droplet
digital
PCR.
individual
miRNA
had
sensitivities
62–71%
specificities
61–79%
for
diagnosing
A
panel
or
produced
79–85%
74–82%.
An
integromic
signature
consisting
two
three
a
higher
sensitivity
(87%)
specificity
(89%)
compared
biomarkers.
signature’s
diagnostic
value
was
confirmed
validation
cohort
56
59
controls,
independent
tumor
stage,
histological
type,
demographic
factors.
Integrating
Язык: Английский