Prognostic relevance of MDK and TIMP1 with immune infiltration in lung adenocarcinoma DOI Creative Commons
Qinghua Zhu, Qingqing Huang, Xiaohua He

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Окт. 17, 2024

Abstract Background LUAD is a prevalent and deadly lung cancer type. MDK TIMP1 expression shows variations in different cancers. The specific contributions of these proteins to progression tumor immunity, however, are not well delineated. Methods We leveraged RNA-seq data from TCGA applied ggpubr R package discern the disparity normal versus tissues. levels were further validated by qRT-PCR western blot. Subsequently, patients stratified into high low groups based on expression, impact their overall survival (OS), disease-free interval (DFI), progression-free (PFI), disease-specific (DSS) was analyzed. Kaplan-Meier curves receiver operation characteristic plotted. also explored KEGG GO annotations for 50 genes exhibiting profiles akin TIMP1, constructed gene-gene interaction network using GeneMANIA. enrichment DEGs pathways scrutinized both TIMP1. Furthermore, we investigated mutational landscape within assessed correlation between infiltration immune cells. Results found be markedly overexpressed LUAD. with diminished have extended OS, DFI, DSS, PFI. Area under curve values 0.943 0.875, respectively. Regression analysis identified as risk factor influencing OS patients. Genes similar notably enriched Proteasome pathway peptidase activator activity, while those exhibit patterns predominantly involved endopeptidase activity Cytoskeleton muscle cells pathway. Functional predictions showed parallel, particularly regulation activity. Mutations determinants There negative purity. dysfunction exclusion score elevated group expression. IPS_ctla_pos IPS_pd1_pos scores statistically significant group. Infiltration immune-related functions more substantial groups. Conclusion A strong exists prognosis LUAD, extent cell infiltration, indicating that targeting related immunotherapy could clinical value.

Язык: Английский

Knockdown of integrin β1 inhibits proliferation and promotes apoptosis in bladder cancer cells DOI

Jin‐feng Wang,

Jianshe Wang, Yang Liu

и другие.

BioFactors, Год журнала: 2024, Номер 51(1)

Опубликована: Дек. 7, 2024

Abstract Bladder cancer (BC) is the most common urinary tract malignancy. Identifying biomarkers that predict prognosis and immune function in patients with BC can enhance our understanding of its pathogenesis provide valuable guidance for diagnosis treatment. Our findings indicate increased ITGB1 expression associated higher clinical grade stage, establishing as an independent prognostic risk factor BC. Enrichment analysis revealed was linked to extracellular matrix. The experimental results showed knockdown cell lines 5637 RT112 reduced their proliferation, migration, invasion. Furthermore, suppression promotes apoptosis cells by inhibiting PI3K‐AKT pathway. A model incorporating CES1, NTNG1, SETBP1, AIFM3 developed based on ITGB1, this accurately patient immunological status. In conclusion, study shows restrain migratory invasive capabilities accelerate apoptosis, role might be PI3K‐AKT, highlighting potential a diagnostic marker therapeutic target

Язык: Английский

Процитировано

19
Pathogenetic development, diagnosis and clinical therapeutic approaches for liver metastasis from colorectal cancer (Review) DOI Creative Commons
Zhenhua Jin,

Yin Li,

Yi Hao

и другие.

International Journal of Oncology, Год журнала: 2025, Номер 66(3)

Опубликована: Фев. 14, 2025

Colorectal cancer (CRC) is a prevalent malignancy and significant proportion of patients with CRC develop liver metastasis (CRLM), which major contributor to CRC‑related mortality. The present review aimed comprehensively examine the pathogenetic development diagnosis CRLM clinical therapeutic approaches for treatment this disease. molecular mechanisms underlying were discussed, including role tumour microenvironment epithelial‑mesenchymal transition. also highlighted importance early detection current challenges in predicting CRLM. Various strategies reviewed, surgical resection, chemotherapy immunotherapy, potential novel therapies, such as selective internal radiation therapy Traditional Chinese Medicine. Despite recent advancements options, remains challenge due complexity heterogeneity CRC. emphasized need multidisciplinary approach integration emerging therapies improve patient outcomes.

Язык: Английский

Процитировано

1
DNA methyltransferase 3A: A prognostic biomarker and potential target for immunotherapy in gastric cancer DOI Creative Commons

Zhou Wei,

Zhenzhen Kou, Yun Luo

и другие.

Medicine, Год журнала: 2025, Номер 104(7), С. e41578 - e41578

Опубликована: Фев. 14, 2025

DNA methyltransferase 3A (DNMT3A) has been associated with the occurrence or progression of various tumors, including gastric cancer. However, role DNMT3A in efficacy immune-cell infiltration tumor microenvironment and immunotherapy cancer remains less explored. expression level was analyzed using TIMER 2.0, Sangerbox 3.0, The Cancer Genome Atlas database further verified by immunohistochemical staining RT-qPCR. UALCAN, chi-square test, Kaplan–Meier plotter databases were performed to assess correlation clinicopathological characteristics prognosis. GeneMANIA database, STRING R package used construct a co-expression gene network. Gene set enrichment analysis identify signaling pathways related expression. correlations between immune infiltrates investigated Plotter, package, TISIDB databases. immunomodulators Immune cell Proportion Score. association mutational burden (TMB), microsatellite instability, dryness evaluated TMB function 2.0. Finally, biological cells assessed CCK-8, cloning formation, transwell assay. remarkably upregulated high poor clinical features survival patients Moreover, analyses showed that its genes involved promoted influencing microenvironment. significantly tumor-infiltrating cells, immunomodulators, TMB, checkpoints knockdown reduced proliferation migration cells. Our findings highlight potential as prognosis biomarker an immunotherapeutic target for

Язык: Английский

Процитировано

0
Single cell analysis reveals that SPP1+ macrophages enhance tumor progression by triggering fibroblast extracellular vesicles DOI
Haocheng Wang, Bowen Qiu, Xinyu Li

и другие.

Translational Oncology, Год журнала: 2025, Номер 55, С. 102347 - 102347

Опубликована: Март 13, 2025

Язык: Английский

Процитировано

0
Hydrogel systems for spatiotemporal controlled delivery of immunomodulators: engineering the tumor immune microenvironment for enhanced cancer immunotherapy DOI Creative Commons
Yanting Liu, Fang Liu, Yan Zeng

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Дек. 13, 2024

Tumor immunotherapy, modulating innate and adaptive immunity, has become an important therapeutic strategy. However, the tumor immune microenvironment’s (TIME) complexity heterogeneity challenge immunotherapy. Hydrogel is a hydrophilic three-dimensional (3D) mesh structure with good biocompatibility drug release control, which widely used in delivery, agriculture, industry, etc. Hydrogels loaded cells, cytokines, checkpoint inhibitors, anti-tumor drugs can achieve targeted delivery ultimately activate response TIME. In this review, we will summarize components of TIME their effects, emerging immunomodulatory agents, characteristics functions hydrogels, how hydrogels regulate cells

Язык: Английский

Процитировано

2
Prognostic relevance of MDK and TIMP1 with immune infiltration in lung adenocarcinoma DOI Creative Commons
Qinghua Zhu, Qingqing Huang, Xiaohua He

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Окт. 17, 2024

Abstract Background LUAD is a prevalent and deadly lung cancer type. MDK TIMP1 expression shows variations in different cancers. The specific contributions of these proteins to progression tumor immunity, however, are not well delineated. Methods We leveraged RNA-seq data from TCGA applied ggpubr R package discern the disparity normal versus tissues. levels were further validated by qRT-PCR western blot. Subsequently, patients stratified into high low groups based on expression, impact their overall survival (OS), disease-free interval (DFI), progression-free (PFI), disease-specific (DSS) was analyzed. Kaplan-Meier curves receiver operation characteristic plotted. also explored KEGG GO annotations for 50 genes exhibiting profiles akin TIMP1, constructed gene-gene interaction network using GeneMANIA. enrichment DEGs pathways scrutinized both TIMP1. Furthermore, we investigated mutational landscape within assessed correlation between infiltration immune cells. Results found be markedly overexpressed LUAD. with diminished have extended OS, DFI, DSS, PFI. Area under curve values 0.943 0.875, respectively. Regression analysis identified as risk factor influencing OS patients. Genes similar notably enriched Proteasome pathway peptidase activator activity, while those exhibit patterns predominantly involved endopeptidase activity Cytoskeleton muscle cells pathway. Functional predictions showed parallel, particularly regulation activity. Mutations determinants There negative purity. dysfunction exclusion score elevated group expression. IPS_ctla_pos IPS_pd1_pos scores statistically significant group. Infiltration immune-related functions more substantial groups. Conclusion A strong exists prognosis LUAD, extent cell infiltration, indicating that targeting related immunotherapy could clinical value.

Язык: Английский

Процитировано

0