Indus journal of bioscience research.,
Год журнала:
2024,
Номер
3(1), С. 868 - 880
Опубликована: Дек. 31, 2024
This
research
sought
to
explore
the
role
of
epigenetic
changes
in
cancer
initiation
and
progression,
therapeutic
potential
modulators,
i.e.,
DNMT
inhibitors
HDAC
inhibitors,
expression
levels
non-coding
RNAs,
miRNAs,
controlling
cells.
The
used
a
quantitative
method,
structured
questionnaires
regression
analysis
determine
biology.
validated
that
65%
respondents
were
familiar
with
DNA
methylation,
60%
histone
modification,
55%
gene-editing
technologies
like
CRISPR.
Chi-Square
presence
significant
disparity
awareness
among
demographic
groups,
p-values
0.02
0.05
for
methylation
by
age
modification
medical
specialty,
respectively.
Regression
significantly
caused
cell
apoptosis
(β
=
0.55,
p
0.0002)
suppressed
migration
-0.30,
0.02),
while
also
0.47,
0.005)
promoted
inhibition
-0.25,
0.03).
test
miRNAs
further
correlations
between
miR-21
type
(χ²
10.4,
0.02)
miR-34a
12.1,
0.01).
These
results
suggest
therapies
miRNA-based
behavior
enhancing
efficacy.
study
highlights
modifications
cancer,
urging
on
optimization.
Pharmacological Research,
Год журнала:
2024,
Номер
208, С. 107354 - 107354
Опубликована: Авг. 17, 2024
Breast
cancer
is
a
major
public
health
concern
worldwide,
being
the
most
commonly
diagnosed
among
women
and
leading
cause
of
cancer-related
deaths.
Recent
studies
have
highlighted
significance
non-histone
methylation
in
breast
cancer,
which
modulates
activity,
interaction,
localization,
stability
target
proteins.
This
regulation
affects
critical
processes
such
as
oncogenesis,
tumor
growth,
proliferation,
invasion,
migration,
immune
responses.
review
delves
into
enzymes
responsible
for
methylation,
protein
arginine
methyltransferases
(PRMTs),
lysine
(KMTs),
demethylases,
explores
their
roles
cancer.
By
elucidating
molecular
mechanisms
functional
consequences
this
aims
to
provide
insights
novel
therapeutic
strategies
targeting
these
pathways.
The
potential
overcome
drug
resistance
enhance
treatment
efficacy
also
discussed,
highlighting
promising
avenues
future
research
clinical
applications.
General
Background:
Breast
cancer
is
the
most
prevalent
affecting
women,
with
increasing
incidence
worldwide.
Specific
Recent
research
has
focused
on
role
of
epigenetic
changes
in
DNA
damage,
repair
mechanisms,
and
potential
therapeutic
effects
probiotics.
Probiotics
have
shown
promise
promoting
tissue
regeneration
repair.
Knowledge
Gap:
However,
precise
impact
probiotics
cells,
specifically
breast
remains
underexplored.
Aims:
This
study
aimed
to
evaluate
damage
AMJ13
Iraqi
cells
assess
cytotoxic
these
cells.
Results:
Using
comet
assay,
we
found
significant
increases
treated
Lactobacillus
plantarum
(T1)
a
combination
eight
probiotic
strains
(T2).
Exposure
T1
for
48
hours
resulted
tail
(P≤0.001),
head
moment
(P<0.001),
while
T2
showed
similar
at
72
(P<0.05).
Image
analysis
further
supported
probiotics,
as
indicated
by
small
curve
Novelty:
provides
novel
insights
into
treatment
demonstrating
their
capacity
enhance
mechanisms
Implications:
The
findings
suggest
that
therapy
may
be
promising
adjunct
cancer,
offering
new
avenue
management
through
enhancement
reduction
damage.
Highlights:
significantly
repaired
enhanced
within
48-72
hours.
offer
therapy.
Keywords:
repair,
cytotoxicity
Next frontier.,
Год журнала:
2024,
Номер
8(1), С. 199 - 199
Опубликована: Ноя. 28, 2024
Epigenetic
modifications
play
a
pivotal
role
in
cellular
differentiation
and
the
onset
of
complex
diseases
by
regulating
gene
expression
without
altering
underlying
DNA
sequence.
Mechanisms
such
as
methylation,
histone
modification,
chromatin
remodeling
orchestrate
transition
from
pluripotent
stem
cells
to
specialized
cell
types,
ensuring
tissue-specific
functionality.
This
research
delves
into
intricate
interplay
between
these
epigenetic
regulators
their
involvement
developmental
processes.
Moreover,
it
examines
how
aberrations
landscapes
contribute
pathogenesis
cancer,
neurodegenerative
disorders,
autoimmune
conditions.
By
employing
advanced
techniques
like
single-cell
epigenomics
CRISPR-based
editing,
this
study
aims
uncover
novel
biomarkers
therapeutic
targets.
The
findings
highlight
dynamic
reversible
nature
marks,
offering
promising
avenues
for
personalized
medicine.
Understanding
disease
development
is
essential
advancing
diagnostics,
therapeutics,
our
broader
understanding
human
biology.
Advances in molecular oncology,
Год журнала:
2024,
Номер
11(4), С. 66 - 79
Опубликована: Дек. 9, 2024
Introduction.
Breast
cancer
(BC)
represents
a
group
of
malignant
neoplasms
with
various
molecular
profiles,
which
are
characterized
by
aberrations
in
the
mechanisms
epigenetic
transcription
regulation.
One
these
disruptions
associated
worse
prognosis
is
overexpression
BET
protein
family,
responsible
for
interaction
factors
histone-rich
acetylated
regions.
Previously,
multitargeted
epigenetically
active
agent
curaxin
CBL0137
(CBL),
we
have
shown
ability
to
inhibit
expression
BRD2,
BRD3,
BRD4
proteins
HeLa
TI
cells
and
BC
cells.
Aim.
To
analyze
action
on
vitro
,
including:
1)
assessment
cytotoxicity,
2)
analysis
effect
cell
cycle,
3)
trigger
apoptosis,
4)
cause
DNA
damage,
5)
genes
involved
proliferation,
apoptosis
repair
processes.
Materials
methods.
The
cytotoxicity
CBL
(MCF7,
MDA-MB-231,
SKBR3)
was
assessed
using
MTT
assay.
cycle
activation
analyzed
flow
cytometry.
Analysis
damage
under
performed
comet
Changes
were
evaluated
real-time
polymerase
chain
reaction.
Results.
half
maximal
inhibitory
concentration
(IC
50
)
1
μM
at
72-hour
exposure,
14–25
24-hour.
Curaxin
(0.5
μM)
caused
G2/M
arrest,
also
triggered
all
lines.
Under
CBL,
an
increase
degree
MCF7
SKBR3
recorded,
both
concentrations
MDA-MB-231
gene
profile
proliferation
corresponded
arrest
phase
cycle.
Also,
p53-dependent
occurred.
An
pro-apoptotic
decrease
anti-apoptotic
shown.
Conclusion.
showed
differential
effects
processes
We
identified
cytostatic
compound,
confirmed
levels.
data
obtained
indicate
tumor
cells,
makes
it
potentially
interesting
combination
therapy.
Indus journal of bioscience research.,
Год журнала:
2024,
Номер
3(1), С. 868 - 880
Опубликована: Дек. 31, 2024
This
research
sought
to
explore
the
role
of
epigenetic
changes
in
cancer
initiation
and
progression,
therapeutic
potential
modulators,
i.e.,
DNMT
inhibitors
HDAC
inhibitors,
expression
levels
non-coding
RNAs,
miRNAs,
controlling
cells.
The
used
a
quantitative
method,
structured
questionnaires
regression
analysis
determine
biology.
validated
that
65%
respondents
were
familiar
with
DNA
methylation,
60%
histone
modification,
55%
gene-editing
technologies
like
CRISPR.
Chi-Square
presence
significant
disparity
awareness
among
demographic
groups,
p-values
0.02
0.05
for
methylation
by
age
modification
medical
specialty,
respectively.
Regression
significantly
caused
cell
apoptosis
(β
=
0.55,
p
0.0002)
suppressed
migration
-0.30,
0.02),
while
also
0.47,
0.005)
promoted
inhibition
-0.25,
0.03).
test
miRNAs
further
correlations
between
miR-21
type
(χ²
10.4,
0.02)
miR-34a
12.1,
0.01).
These
results
suggest
therapies
miRNA-based
behavior
enhancing
efficacy.
study
highlights
modifications
cancer,
urging
on
optimization.
