Lathyrol inhibits the proliferation of renca cells by affecting the expression of TGF-β/smad pathway and then affecting the cell cycle
Abstract
Background
Renal
cell
carcinoma
(RCC)
is
a
prevalent
malignant
tumor
with
high
morbidity
and
mortality.
The
TGF-β/smad
signaling
pathway
plays
significant
role
in
the
development
progression
of
RCC.
Methods
This
study
explored
impact
lathyrol
on
proliferation
mice
RCC
renca
cells
by
inhibiting
arresting
cycle.
Bioinformatics
analysis,
culture
experiments,
animal
experiments
were
conducted
to
detect
effects
activity,
mRNA,
protein
expressions
xenograft
tumors,
as
well
cycle
proteins
regulatory
proteins.
Results
Lathyrol
treatment
showed
positive
correlation
inhibitory
effect
proliferation.
IC
values
786-O
comparable.
In
vitro,
decreased
mRNA
TGF-β1,
TGF-βR1,
smad2,
smad3,
smad4,
smad9,
while
increasing
smad4.
vivo,
suppressed
smad9
xenografts,
cyclinD1,
cyclinB1,
cyclinA1,
cyclinE1,
CDK6,
CDK4,
CDK1,
P16,
P21,
P27.
Conclusion
can
repress
expression
key
pathway,
impede
signal
transduction,
arrest
cells,
subsequently
inhibit
cells.
Future
studies
are
needed
further
explore
mechanism
treatment.
Research Square (Research Square), Год журнала: 2025, Номер unknown
Опубликована: Март 3, 2025
Язык: Английский