Purification and Functional Characterization of a New Endoglucanase from Pleurotus djamor PLO13 Produced by Solid-State Fermentation of Agro-Industrial Waste

Fermentation, Год журнала: 2025, Номер 11(4), С. 182 - 182

Опубликована: Апрель 1, 2025

The increasing generation of agro-industrial waste and its improper disposal have raised significant environmental concerns, highlighting the urgent need for sustainable alternatives which would repurpose these materials. In this context, enzymes such as endoglucanase play a critical role in degrading lignin–cellulose biomass by catalyzing breakdown β-1,4-glycosidic bonds cellulose, thereby converting it into fermentable sugars with diverse industrial applications. This study aimed to investigate production, purification, characterization an produced fungus Pleurotus djamor PLO13, using coconut fiber, sugarcane bagasse, wheat bran, pineapple crown substrates. Endoglucanase activity was measured Miller method (1959), 2% (w/v) carboxymethyl cellulose (CMC) substrate. Solid-state fermentation (SSF) found be highly efficient enzyme synthesis, bran emerging most effective substrate, yielding production 7.19 U after 120 h cultivation. purified through ethanol precipitation ion-exchange chromatography DEAE-Sepharose, achieving recovery rate 110%, possibly due removal inhibitors present crude extract. exhibited stability across broad pH range thermostability, optimal at 5.0 50 °C. Furthermore, activated EDTA, Mn2+, Ca2+, while being inhibited Mg2+. Notably, demonstrated halotolerance, 60% upon addition 3 M NaCl. Kinetic analysis revealed that showed affinity CMC substrate analyzed parameters (pH °C), Km Vmax values 0.0997 mg/mL 112.2 µg/min/mL, respectively. These findings suggest from P. PLO13 has promising potential biotechnological applications, underscoring feasibility use lignocellulosic substrates processes.

Язык: Английский

Synthesis of nanoparticles using advanced techniques

Next Nanotechnology, Год журнала: 2025, Номер 8, С. 100169 - 100169

Опубликована: Янв. 1, 2025

Язык: Английский