Frontiers in Microbiology, Год журнала: 2024, Номер 15
Опубликована: Дек. 9, 2024
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Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 285, С. 117109 - 117109
Опубликована: Сен. 30, 2024
Язык: Английский
Процитировано
5
Poultry Science, Год журнала: 2025, Номер 104(2), С. 104787 - 104787
Опубликована: Янв. 10, 2025
Escherichia coli (E. coli) is a significant pathogen responsible for intestinal infections and foodborne diseases. The rise of antibiotic resistance poses challenge to global public health. Traditional therapy becoming increasingly ineffective, highlighting the urgent need innovative control strategies. This study explores potential bacteriophages as sustainable alternative traditional antibiotics. From 2021 2022, total 183 non-repetitive duck source fecal samples were collected from Mianyang City, Sichuan Province, 126 strains E. isolated. minimum inhibitory concentration (MIC) test showed that these exhibited high piperacillin (96.8%), tetracycline (88.9%), chloramphenicol (86.5%). It concerning 93.7% isolates are classified multidrug-resistant (MDR), posing threat existing treatment options. 20 isolated soil samples, among which 5 selected further analysis. Bacteriophage YP6 excellent lytic effects on MDR strains, especially strain MY104, well representative serotypes O1 MY51) O18 MY106). identification member Myoviridae family was conducted using transmission electron microscopy, it found have an optimal infection factor 0.1. Bacteriophages exhibit thermal pH stability, maintaining survival at temperatures up 60 °C ranges 4 10. Whole genome sequencing confirmed has double stranded DNA 139,323 base pairs (bp), no or virulence genes found, indicating low possibility horizontal gene transfer. In addition, effectively inhibits formation biofilm, key in chronic infections. vivo experiments Galleria mellonella (G. mellonella) larvae shown protective effect against infection. summary, bacteriophage expected become therapeutic agent due its broad host range, environmental biofilm inhibition properties. Future research should optimize preparations, evaluate safety efficacy animal models, establish clinical application plans field food safety.
Язык: Английский
Процитировано
0
Frontiers in Veterinary Science, Год журнала: 2025, Номер 12
Опубликована: Фев. 5, 2025
Escherichia coli is a major pathogen responsible for calf diarrhea. However, it has developed resistance to many antimicrobial drugs their inappropriate usage. The bacterial quorum sensing system transmits information between bacteria, it's important in regulating virulence, drug and acid so on. This can found Gram-negative bacteria operates through acyl-homoserine lactone (AHL) signaling molecules. In this study, type I AHL, N-Octanoyl-L-Homoserine (C8), was added E. growth medium investigate its regulatory functions resistance. After screening out the strains of that showed an obvious effect ofloxacin (OFX), transcriptomic sequencing performed on from sub-inhibitory concentration group plus C8 group, control group. It shows significantly influenced OFX minimum inhibitory tested strain increased. To Analyze transcriptome results identified 415 differentially expressed genes groups, which 201 were up-regulated 214 down. There 125 treated with those C8, 102 23 Finally, adding increased OFX. Data differently helps explain how controls .
Язык: Английский
Процитировано
0
Frontiers in Microbiology, Год журнала: 2025, Номер 16
Опубликована: Фев. 21, 2025
β-lactam antibiotics are essential medications for treating human diseases. The spread of extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) exists globally in multiple reservoirs and the natural environment poses an immense threat to public health. Plasmid incompatibility groups, such as IncFIA, IncI1, IncY, IncFIB, IncN, IncFIC, IncX4, IncB/O/K/Z, IncHI1/2, IncA/C, which exist humans, animals, environment, carrying bla CTX-M, TEM, SHV genes. ISEcp1 upstream orf477 downstream CTX-M genes, well other mobile genetic elements (MGEs) IS903 IS26, involved capturing mobilizing antibiotic-resistant genes (ARGs). CTX-M-15 gene is most common among all discussed reservoirs. environmental reservoir propagation mode ESBL-PE increasing difficult control. reasons include but not limited bacterial adaptability horizontal transfer (HGT) mediated by MGEs plasmids. Conjugation a pathway HGT that almost uncontrollable. plasmids Tn3, IS1380 families, IncK, IncN facilitating This review highlights need monitor trends antimicrobial resistance (AMR) environment. Therefore, policies antibiotic management plans, training healthcare providers and/or patients, cautious use antibiotics, epidemiological networks, pre-travel consultations, World Health Organization (WHO) infection control biosafety guidelines, intervention measures considered desirable.
Язык: Английский
Процитировано
0
Frontiers in Veterinary Science, Год журнала: 2025, Номер 12
Опубликована: Март 21, 2025
Fosfomycin (FOS) is a critical antibiotic for treating multi-drug resistant (MDR) Enterobacteriaceae infections, but its effectiveness jeopardized by the dissemination of plasmids encoding enzymes that modify FOS. Despite prohibition on use in animal breeding China, 100 strains Escherichia coli ( E. ) exhibiting high resistance to FOS (MIC≥512 mg/L) were isolated from samples waterfowl origin collected Hainan, Sichuan, and Anhui. These commonly carried fos A 3 (88/100, 88.0%). In addition, 21 other antimicrobial genes (ARGs) detected these strains, with positivity rates tet A, aphA1 , sul2 folR qnrS bla CTX-M . It noteworthy there was significant positive correlation between A3 (OR = 15.162, 95% CI: 1.875–122.635). The results pulsed-field gel electrophoresis (PFGE) demonstrated existence multiple dispersed clonal clusters. Multilocus sequence typing (MLST) analysis identified 45 ST types, ST48 ST10 representing most dominant clones. conjugation experiments, 53 A-like transconjugants obtained measurable frequency, which strongly may mainly locate different types possessing an efficient transmission ability. Whole genome sequencing (WGS) further showed co-localized degree similarity genetic structure. Of particular interest observation frequently accompanied IS 26 either side gene. This structure play pivotal role horizontal transfer A3. study revealed alarming prevalence delved deeply into characteristics mechanisms discovery plasmid-mediated, transmissible poses threat “One Health”. There’s urgent need thorough monitoring control measures against resistance.
Язык: Английский
Процитировано
0
Veterinary Microbiology, Год журнала: 2025, Номер unknown, С. 110492 - 110492
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
The Science of The Total Environment, Год журнала: 2025, Номер 976, С. 179321 - 179321
Опубликована: Апрель 9, 2025
Язык: Английский
Процитировано
0
PLoS ONE, Год журнала: 2025, Номер 20(4), С. e0321514 - e0321514
Опубликована: Апрель 11, 2025
The rapid spread of extended-spectrum β-lactamases (ESBLs)-producing Escherichia coli (ESBL-EC) around the world has become a significant challenge for humans and animals. In this study, we aimed to examine characteristics horizontal gene transfer (HGT) capacity ESBL-EC derived from captive primates. We screened among total 444 multidrug-resistant (MDR) E. strains isolated 13 zoos in China using double-disk test. ESBL genes, mobile genetic elements (MGEs), virulence-associated genes (VAGs) were detected through polymerase chain reaction (PCR). Furthermore, conjugation experiments conducted HGT ESBL-EC, population structure (phylogenetic groups MLST) was determined. Our results showed that 69 (15.54%, 69/444) identified, 5 variants bla CTX 3 TEM detected. highest detection rate CTX-M-55 (49.28%, 34/69), followed by CTX-M-15 (39.13%, 27/69). Ten MGEs most prevalent IS26 (78.26%, 54/69), ISEcp1 (60.87%, 42/69). Eighteen combinations detected, which + predominant (18.84%, n = 13). A 15 VAGs fimC (84.06%, 58/69), sitA 54/69). ability analysis 40.58% (28/69) exhibited engage conjugative transfer. Plasmid typing revealed IncFIB (78.57%, 22/28) had rates. antibiotic resistance (ARGs) TEM-135 , tetA qnrS ; trbC ISCR3/14 high rates phylogroup B1 ST2161 prevalent. poses potential threat primates may other animals, humans, environment. It is imperative implement measures prevent transmission
Язык: Английский
Процитировано
0
Biodiversitas Journal of Biological Diversity, Год журнала: 2024, Номер 25(3)
Опубликована: Апрель 8, 2024
Abstract. Kendek IA, Putri MFR, Wibisono FJ, Effendi MH, Tyasningsih W, Ugbo EN, Agumah NB. 2024. Molecular detection of hlyF gene on multidrug resistance avian pathogenic Escherichia coli isolated from ducks wet markets Surabaya, Indonesia. Biodiversitas 25: 1246-1252. Avian Pathogenic (APEC) is a strain pathogen that can cause colibacillosis in poultry, including worldwide. This disease be influenced by various virulence genes, one which the systemic disorders and potentially become zoonotic poultry. study aimed to detect (MDR) properties originating 158 duck cloacal swab samples seven live Surabaya. Samples were identified using Eosin Methylene Blue Agar (EMBA) MacConkey (MCA) media, Gram staining, then continued with biochemical tests Triple Sugar Iron (TSIA), Simmons Citrate (SCA), Sulfide Indole Motility (SIM), Methyl red (MR) Voges-Proskauer (VP). tested for MDR confirmation PCR. Based results this study, it showed 85% (134/158) positive coli. bacteria experienced antibiotic erythromycin 96% (129/134), ciprofloxacin 16% (22/134), antibiotics gentamicin 15% (20/134), aztreonam 4.4% (6/134) chloramphenicol 3% (5/58). The test while PCR 60% (12/20). Therefore, was found have gene, an at Surabaya market. Furthermore, APEC strains MDR, affect public health.
Язык: Английский
Процитировано
1
Frontiers in Microbiology, Год журнала: 2024, Номер 15
Опубликована: Дек. 9, 2024
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Процитировано
1