Intestinal Microbiota and High-Risk Antibiotic Resistance Genes in Wild Birds with Varied Ecological Traits: Insights from Opportunistic Direct Sampling in Tianjin, China DOI
L. Tong,

Yetong Shao,

Xiaoke Pang

и другие.

Опубликована: Янв. 1, 2024

Within One Health framework, the dissemination of antibiotic resistance genes (ARGs) and pathogenic bacteria by wild birds has attracted increasing attention. In this study, gut samples opportunistically collected in Tianjin, China, situated along East Asian-Australasian Flyway, were used to ascertain realistic distribution ARGs their intestinal tracts. These have different dietary habits (herbivore, carnivore, omnivore) residency statuses (resident migratory birds). Using 16S rRNA gene sequencing qPCR, we analyzed microbial communities abundance high-risk mobile genetic elements (MGEs). Birds with distinct ecological traits exhibited significant variations bacterial composition, yet similar diversity. Shigella sp. emerged as core pathogen, a mean relative 2.57 1,466 times higher than that other bacteria, its concentration correlated host's trophic level indicated δ15N values. The MGEs also varied bird traits. All 10 targeted detected carnivores or passage migrants, while carried significantly greater intI1 residents (p < 0.05). potential harbor disseminate cannot be ignored. Network analysis revealed blaTEM-1 presence multiple microorganisms, positively associated Clostridioides difficile, emphasizing risk potential. dfrA12 transmission across levels facilitated was observed. Our study underscores high posed carrying importance further research surveillance field.

Язык: Английский

Effects of dietary tea polyphenols on intestinal microflora and metabonomics in juvenile hybrid sturgeon (Acipenser baerii♀ × A. schrenckii ♂) DOI Creative Commons
Zhenxin Zhao, Qihui Yang,

Beiping Tan

и другие.

Aquaculture Reports, Год журнала: 2024, Номер 35, С. 102020 - 102020

Опубликована: Март 11, 2024

The present study aimed to evaluate the role of modulatory effect Tea polyphenols (TPs) on intestinal microbiota and its metabolism juvenile hybrid sturgeon. A total 450 fish (97.20 ± 0.18 g) were fed five diets with different concentrations TPs mg / kg: 0 (TP-0), 100 (TP-100), 300 (TP-300), 500 (TP-500) 1000 (TP-1000) for 56 days. results showed that dietary kg significantly increased villus height (VH), crypt depth (CD) number goblet cells (GC) in intestine (p < 0.05), mg/kg supplementation decreased muscle thickness (MT) width (VW) compared TP-0 group 0.05). With increase TPs, relative abundance potential probiotics including Lactobacillus Rhodobacteraceae increased, while pathogens especially Clostridiaceae decreased. And at could be beneficial self-colonization microflora Metabonomics analysis differential amino acids metabolites pathways enriched TPs. highest intestines sturgeon tryptophan metabolism, protein digestion absorption kg. These provide a novel insight into immune-protective regulating which are helpful thoroughly understanding function providing solid basis deeper utilization as natural feed additives.

Язык: Английский

Процитировано

5

Prevention of Clostridium Perfringens induced chicken NE by probiotics -A Review DOI Creative Commons

Ainul Zahra,

Gerui Zhang, Mingyue Wang

и другие.

Animals and zoonoses., Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0

Full-length 16S rRNA sequencing revealed an altered microbiome diversity and composition of the jejunum and cecum in chicken infected with Eimeria necatrix DOI

Nian-Yu Xue,

Qianqian Feng, Yu Zhu

и другие.

Veterinary Parasitology, Год журнала: 2025, Номер unknown, С. 110458 - 110458

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Bacillus subtilis fmbj ameliorates lipopolysaccharide-induced intestinal dysfunction in broilers by enhancing the SIRT1/PGC1α pathway DOI Creative Commons

Luyi Jiang,

Kaiwen Bai,

Tian Wang

и другие.

Poultry Science, Год журнала: 2024, Номер 103(8), С. 103964 - 103964

Опубликована: Июнь 12, 2024

This study aimed to explore the impact of dietary Bacillus subtilis fmbj (BS) supplementation on acute intestinal dysfunction induced by lipopolysaccharide (LPS) in broilers. One hundred and eighty 1-day-old male Arbor Acres broilers were randomly divided into three treatment groups, each comprising ten replicates 6 birds. On d 20, LPS-challenged (LPS group LPS-BS group) LPS-unchallenged (CON received intraperitoneal injections 1 mg/kg body weight LPS solution an equivalent volume sterile saline, respectively. Compared CON group, disrupted (P < 0.05) morphology small intestine (jejunum or ileum), exacerbated serum, intestinal, mitochondrial antioxidant capacity, oxidative damage, altered expression genes proteins related antioxidants, cell adhesion, function jejunum. The exhibited a tendency towards improvement morphology, jejunum when compared group. In conclusion, BS may confer protection against LPS-induced enhancing activation SIRT1/PGC1α, suggesting its potential as valuable additive for poultry industry.

Язык: Английский

Процитировано

2

Intestinal microbiota and high-risk antibiotic resistance genes in wild birds with varied ecological traits: Insights from opportunistic direct sampling in Tianjin, China DOI
L. Tong,

Yetong Shao,

Xiaoke Pang

и другие.

Environmental Research, Год журнала: 2024, Номер 263, С. 120040 - 120040

Опубликована: Сен. 20, 2024

Язык: Английский

Процитировано

2

In-ovo injection of Bacillus subtilis, raffinose, and their combinations enhances hatchability, gut health, nutrient transport- and intestinal function-related genes, and early development of broiler chicks DOI Creative Commons
Abdelrazeq M. Shehata,

Nermien Helmy Seddek,

Tarek Khamis

и другие.

Poultry Science, Год журнала: 2024, Номер 103(11), С. 104134 - 104134

Опубликована: Июль 30, 2024

An experiment was conducted to assess the response of chicks in-ovo injection Bacillus subtilis (probiotic), raffinose (prebiotic), and their combinations. The study used 1,500 embryonated eggs allotted 10 groups/ 6 replicates (150 eggs/group). experimental treatments were: 1) un-injected control (NC); 2) sham (sterile distilled water) (PC); 3) probiotic 4 ×

Язык: Английский

Процитировано

1

Citric Acid by-Product Fermentation by Bacillus subtilis I9: A Promising Path to Sustainable Animal Feed DOI Creative Commons
Sirisak Tanpong, Nalisa Khochamit, Padsakorn Pootthachaya

и другие.

Veterinary Sciences, Год журнала: 2024, Номер 11(10), С. 484 - 484

Опубликована: Окт. 8, 2024

Citric acid by-products in animal feed pose a sustainability challenge.

Язык: Английский

Процитировано

1

Intestinal Microbiota and High-Risk Antibiotic Resistance Genes in Wild Birds with Varied Ecological Traits: Insights from Opportunistic Direct Sampling in Tianjin, China DOI
L. Tong,

Yetong Shao,

Xiaoke Pang

и другие.

Опубликована: Янв. 1, 2024

Within One Health framework, the dissemination of antibiotic resistance genes (ARGs) and pathogenic bacteria by wild birds has attracted increasing attention. In this study, gut samples opportunistically collected in Tianjin, China, situated along East Asian-Australasian Flyway, were used to ascertain realistic distribution ARGs their intestinal tracts. These have different dietary habits (herbivore, carnivore, omnivore) residency statuses (resident migratory birds). Using 16S rRNA gene sequencing qPCR, we analyzed microbial communities abundance high-risk mobile genetic elements (MGEs). Birds with distinct ecological traits exhibited significant variations bacterial composition, yet similar diversity. Shigella sp. emerged as core pathogen, a mean relative 2.57 1,466 times higher than that other bacteria, its concentration correlated host's trophic level indicated δ15N values. The MGEs also varied bird traits. All 10 targeted detected carnivores or passage migrants, while carried significantly greater intI1 residents (p < 0.05). potential harbor disseminate cannot be ignored. Network analysis revealed blaTEM-1 presence multiple microorganisms, positively associated Clostridioides difficile, emphasizing risk potential. dfrA12 transmission across levels facilitated was observed. Our study underscores high posed carrying importance further research surveillance field.

Язык: Английский

Процитировано

0