Regulatory role of N6-Methyladenosine on skeletal muscle development in Hu sheep DOI Creative Commons

Junfang Jiang,

Liangyong Guo,

Xin Huang

и другие.

Frontiers in Genetics, Год журнала: 2024, Номер 15

Опубликована: Авг. 21, 2024

N6-Methyladenosine (m6A) RNA modification plays an essential role in many biological processes. To investigate the regulatory of m6A on skeletal muscle development Hu sheep, this study took newborn sheep (b_B Group) and six-month-old (s_B as objects. MeRIP-seq RNA-Seq analysis techniques were used to detect differentially methylated genes (DMGs) expressed (DEGs) longissimus dorsi at different months age. Then, conjoint was further employed screen for key involved that are modified by mRNA. According results analysis, there 285 peaks (DMPs) total between b_B Group s_B Group, with 192 significant upregulated 93 downregulated peaks. GO KEGG revealed DMGs mainly enriched actin-binding, cellular transport, metabolic pathways. RNA-seq 4,349 DEGs 2010 2,339 genes. found be regulation actin cytoskeleton tissue, AMPK FoxO signaling pathways, etc. The demonstrated 283 both Among them, three relevant growth (RGMB, MAPK8IP3, RSPO3) selected candidates quantitative validation, line sequencing results. mentioned above all suggest a certain sheep.

Язык: Английский

The m6A Modification Regulates the Composition of Myofiber Types in Chicken Skeletal Muscle DOI Creative Commons

Yanju Shan,

Yifan Liu, Ming Zhang

и другие.

Poultry Science, Год журнала: 2025, Номер unknown, С. 104811 - 104811

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
The landscape of RNA 5-methylcytosine modification during chicken embryonic myogenesis DOI Creative Commons
Hong‐Xia Jiang, Jing Liu, Yuxiang Wang

и другие.

Poultry Science, Год журнала: 2025, Номер unknown, С. 105109 - 105109

Опубликована: Март 1, 2025

Skeletal muscle is a vital protein source for human diets, making its development significant focus in poultry research. This study examines the effects of RNA 5-methylcytosine (m5C) modifications on chicken skeletal at two critical embryonic stages: before myoblast differentiation (E10) and after (E19). By employing MeRIP-seq, we analyzed dynamic distribution m5C within leg transcriptome, uncovering notable differences modification states between these stages. Our results indicate that are widespread transcriptome present unique pattern. Unlike N6-methyladenosine modifications, which typically show negative correlation with expression, exhibited weak positive our findings. Additionally, identified multiple peaks important epigenetic regulators, including DNMT3A, DNMT3B, DNMT1, TET3, METTL3. observation suggests may interact genes to jointly influence development. Furthermore, key linked enriched pathways associated cell cycle regulation, growth, lipid metabolism. provides valuable insights into role highlights their potential coordinating myogenesis metabolic processes.

Язык: Английский

Процитировано

0
METTL3-dependent m6A modification of GHR mRNA regulates mitochondrial function through mitochondrial biogenesis during myoblast differentiation DOI Creative Commons
Changbin Zhao,

Bowen Hu,

Zhijun Wang

и другие.

Poultry Science, Год журнала: 2025, Номер 104(7), С. 105216 - 105216

Опубликована: Апрель 25, 2025

Язык: Английский

Процитировано

0
Genome-wide re-sequencing reveals selection signatures for important economic traits in Taihang chickens DOI Creative Commons
Ran Zhang, Wenjun Wang, Zhenhong Zhang

и другие.

Poultry Science, Год журнала: 2024, Номер 103(11), С. 104240 - 104240

Опубликована: Авг. 22, 2024

Taihang chickens is precious genetic resource with excellent adaptability and disease resistance, as well high-quality eggs meat. However, the mechanism underlying important economic traits remain largely unknown. To address this gap, we conducted whole-genome resequencing of 66 15 White Plymouth rock chicken (Baiyu). The population structure analysis revealed that Baiyu are 2 independent populations. genomic regions strong selection signals some candidate genes related to appearance were identified. Additionally, found a continuously selected 1.2 Mb region on chromosome closely resistance. Therefore, our findings helpful in further understanding architecture provided worthy theoretical basis technological support improve chickens.

Язык: Английский

Процитировано

2
Identification of Key Differentially Methylated Genes Regulating Muscle Development in Chickens: Insights from Jingyuan Breed DOI Creative Commons
Tong Zhang, Jiwei Li,

Jiahuan Hu

и другие.

Poultry Science, Год журнала: 2024, Номер 103(12), С. 104292 - 104292

Опубликована: Сен. 6, 2024

Язык: Английский

Процитировано

2
Regulatory role of N6-Methyladenosine on skeletal muscle development in Hu sheep DOI Creative Commons

Junfang Jiang,

Liangyong Guo,

Xin Huang

и другие.

Frontiers in Genetics, Год журнала: 2024, Номер 15

Опубликована: Авг. 21, 2024

N6-Methyladenosine (m6A) RNA modification plays an essential role in many biological processes. To investigate the regulatory of m6A on skeletal muscle development Hu sheep, this study took newborn sheep (b_B Group) and six-month-old (s_B as objects. MeRIP-seq RNA-Seq analysis techniques were used to detect differentially methylated genes (DMGs) expressed (DEGs) longissimus dorsi at different months age. Then, conjoint was further employed screen for key involved that are modified by mRNA. According results analysis, there 285 peaks (DMPs) total between b_B Group s_B Group, with 192 significant upregulated 93 downregulated peaks. GO KEGG revealed DMGs mainly enriched actin-binding, cellular transport, metabolic pathways. RNA-seq 4,349 DEGs 2010 2,339 genes. found be regulation actin cytoskeleton tissue, AMPK FoxO signaling pathways, etc. The demonstrated 283 both Among them, three relevant growth (RGMB, MAPK8IP3, RSPO3) selected candidates quantitative validation, line sequencing results. mentioned above all suggest a certain sheep.

Язык: Английский

Процитировано

0