Highly Sensitive Assays for detection of headache inducing neuropeptides, Pituitary adenylate cyclase-activating polypeptide (PACAP) and Calcitonin gene-related peptide (CGRP). DOI

Ourania Tzara,

Josefine Nielsen Søderberg,

Justyna M.C. Bahl

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 6, 2025

Migraine is one of the most disabling diseases that continues to pose a significant societal burden. Although there are now treatment options for people with migraine, it remains challenging identify them as clinical features diverse and complex, no validated diagnostic or prediction biomarkers. Identification based on either coding use certain acute headache abortive treatments. However, socioeconomic disparities can contribute under-diagnosis under-treatment migraine. Thus, efforts find biomarkers individuals migraine which variables could explain migraine-related chronification disability warranted. We aimed investigate levels inducing neuropeptides; calcitonin gene-related peptide (CGRP) pituitary adenylate cyclase-activating polypeptide (PACAP) in peripheral blood samples potential developed highly sensitive assays CGRP PACAP MSD S-PLEX assay platform used bioanalysis preclinical samples. Wildtype neuropeptide challenged mice rats were profiled using assay. To follow-up, commercially obtained plasma from healthy controls migraineurs initially profiled. Subsequently, we (during after attack controls. Both transferred Celerion where they analysis Using assay, able reliably measure circulating endogenous administrated PACAP38in mouse rat plasma. Additionally, Furthermore, initial human samples, not significantly different compared patients. ≥50% showed increased during their period post attack. Overall, 3 - 396% increase both neuropeptides Circulating control subjects consistent previously measured levels. Our successful measuring PACAP38 qualified measurement Plasma elevated an period, may help differentiation non-Migraineurs, amongst migraines best each patient.

Язык: Английский

Highly Sensitive Assays for detection of headache inducing neuropeptides, Pituitary adenylate cyclase-activating polypeptide (PACAP) and Calcitonin gene-related peptide (CGRP). DOI

Ourania Tzara,

Josefine Nielsen Søderberg,

Justyna M.C. Bahl

и другие.

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 6, 2025

Migraine is one of the most disabling diseases that continues to pose a significant societal burden. Although there are now treatment options for people with migraine, it remains challenging identify them as clinical features diverse and complex, no validated diagnostic or prediction biomarkers. Identification based on either coding use certain acute headache abortive treatments. However, socioeconomic disparities can contribute under-diagnosis under-treatment migraine. Thus, efforts find biomarkers individuals migraine which variables could explain migraine-related chronification disability warranted. We aimed investigate levels inducing neuropeptides; calcitonin gene-related peptide (CGRP) pituitary adenylate cyclase-activating polypeptide (PACAP) in peripheral blood samples potential developed highly sensitive assays CGRP PACAP MSD S-PLEX assay platform used bioanalysis preclinical samples. Wildtype neuropeptide challenged mice rats were profiled using assay. To follow-up, commercially obtained plasma from healthy controls migraineurs initially profiled. Subsequently, we (during after attack controls. Both transferred Celerion where they analysis Using assay, able reliably measure circulating endogenous administrated PACAP38in mouse rat plasma. Additionally, Furthermore, initial human samples, not significantly different compared patients. ≥50% showed increased during their period post attack. Overall, 3 - 396% increase both neuropeptides Circulating control subjects consistent previously measured levels. Our successful measuring PACAP38 qualified measurement Plasma elevated an period, may help differentiation non-Migraineurs, amongst migraines best each patient.

Язык: Английский

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