International Journal of Food Engineering,
Год журнала:
2025,
Номер
21(5), С. 285 - 303
Опубликована: Май 1, 2025
Abstract
Lipase
has
emerged
as
a
promising
eco-friendly
biocatalyst.
However,
its
free-form
application
is
limited
by
challenges,
such
poor
recovery
and
stability.
Immobilization
presents
an
effective
strategy
to
overcome
these
limitations,
thereby
enhancing
catalytic
performance.
Nevertheless,
lipase
immobilization
not
obstacle-free
either
necessitates
comprehensive
solutions.
The
development
of
novel
techniques
matrix
materials
remains
relatively
underexplored.
This
review
offers
analysis
both
traditional
advanced
enzyme
strategies,
with
focus
on
how
different
carrier
influence
enzymatic
properties
efficiency.
By
discussing
the
diverse
industrial
applications
immobilized
lipases
while
also
outlining
potential
research
trajectories
within
this
domain,
aims
establish
robust
theoretical
framework
for
improving
performance
enzymes.
Furthermore,
innovative
technologies
3D
printing
membrane-based
are
expected
drive
future
advancements,
providing
strong
foundation
engineering
lipases.
Preparative Biochemistry & Biotechnology,
Год журнала:
2025,
Номер
unknown, С. 1 - 10
Опубликована: Апрель 21, 2025
The
present
study
aimed
to
immobilize
an
inulinase
obtained
from
Aspergillus
terreus
URM4658
by
entrapment
in
calcium
alginate
beads.
immobilization
process
yielded
a
satisfactory
yield
(92.72%)
using
1.25%
sodium
and
0.35
M
CaCl2
with
curing
time
of
90
min.
immobilized
enzyme
exhibited
optimum
pH
temperature
at
7.0
60
°C,
respectively,
showing
increased
affinity
for
the
substrate
after
process,
as
evidenced
decrease
Km
compared
its
free
form.
Moreover,
demonstrated
good
thermostability
50
observed
t1/2
(649.83-420.84
min)
D-values
(2158.67-1398.00
min),
respectively.
biocatalyst
also
reusability,
maintaining
92.73%
residual
activity
10
reaction
cycles
no
loss
30
days
storage.
A
continuous
inulin
hydrolysis
operation
packed
bed
reactor
was
performed
inulinase,
maximum
release
total
reducing
sugars
nystose
3.27
0.82
g
L-1,
observed.
results
indicate
that
is
promising
alternative
bioprocess
involving
inulin-rich
feedstocks.
International Journal of Food Engineering,
Год журнала:
2025,
Номер
21(5), С. 285 - 303
Опубликована: Май 1, 2025
Abstract
Lipase
has
emerged
as
a
promising
eco-friendly
biocatalyst.
However,
its
free-form
application
is
limited
by
challenges,
such
poor
recovery
and
stability.
Immobilization
presents
an
effective
strategy
to
overcome
these
limitations,
thereby
enhancing
catalytic
performance.
Nevertheless,
lipase
immobilization
not
obstacle-free
either
necessitates
comprehensive
solutions.
The
development
of
novel
techniques
matrix
materials
remains
relatively
underexplored.
This
review
offers
analysis
both
traditional
advanced
enzyme
strategies,
with
focus
on
how
different
carrier
influence
enzymatic
properties
efficiency.
By
discussing
the
diverse
industrial
applications
immobilized
lipases
while
also
outlining
potential
research
trajectories
within
this
domain,
aims
establish
robust
theoretical
framework
for
improving
performance
enzymes.
Furthermore,
innovative
technologies
3D
printing
membrane-based
are
expected
drive
future
advancements,
providing
strong
foundation
engineering
lipases.
