Bridging imaging-based in vitro methods from biomedical research to regulatory toxicology

Archives of Toxicology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 13, 2025

Язык: Английский

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Oligodendrogenesis and myelination tracing in a CRISPR/Cas9-engineered brain microphysiological system

Frontiers in Cellular Neuroscience, Год журнала: 2023, Номер 16

Опубликована: Янв. 19, 2023

Introduction Oligodendrocytes (OLs) are the myelin-forming cells of central nervous system (CNS). Although OLs can be differentiated from human-induced pluripotent stem (hiPSCs), in vitro modeling axon myelination human remains challenging. Brain microphysiological systems (bMPS, e.g. organoids) complex three-dimensional (3D) cultures that offer an ideal to study this process as differentiate a more vivo -like environment; surrounded by neurons and astrocytes, which support axons. Methods Here, we take advantage CRISPR/Cas9 technology generate hiPSC line proteolipid protein 1 (PLP1), marker, is tagged with super-fold GFP (sfGFP). While generating PLP1-sfGFP reporter, used reverse transfection obtained higher Knock-In (KI) efficiency compared forward (61–72 vs. 46%). Results After validation KI quality control line, selected clones were into bMPS, fidelity, specificity, function PLP verified model. We tracked different stages oligodendrogenesis lines based on + cells’ morphology, presence surrounding axons during bMPS’ differentiation. Finally, challenged bMPS cuprizone quantified changes both percentage expressing intensity expression. Discussion This work demonstrates efficient method for description new 3D model OL differentiation, migration, maturation neurodevelopment well response environmental chemicals or disease-associated stressors.

Язык: Английский

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Recommendations on fit-for-purpose criteria to establish quality management for microphysiological systems and for monitoring their reproducibility

Stem Cell Reports, Год журнала: 2024, Номер 19(5), С. 604 - 617

Опубликована: Апрель 25, 2024

SummaryCell culture technology has evolved, moving from single-cell and monolayer methods to 3D models like reaggregates, spheroids, organoids, improved with bioengineering microfabrication bioprinting. These advancements, termed microphysiological systems (MPSs), closely replicate tissue environments human physiology, enhancing research biomedical uses. However, MPS complexity introduces standardization challenges, impacting reproducibility trust. We offer guidelines for quality management control criteria specific MPSs, facilitating reliable outcomes without stifling innovation. Our fit-for-purpose recommendations provide actionable advice achieving consistent performance.

Язык: Английский

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Intestinal organ chips for disease modelling and personalized medicine

Nature Reviews Gastroenterology & Hepatology, Год журнала: 2024, Номер 21(11), С. 751 - 773

Опубликована: Авг. 27, 2024

Язык: Английский

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干细胞资源库的标准化建设：挑战和机遇

Scientia Sinica Vitae, Год журнала: 2024, Номер unknown

Опубликована: Май 29, 2024

干细胞资源在基础科研和临床转化中的重要性日益凸显,干细胞资源库的标准化建设更是成为推动干细胞与再生医学领域规范发展的关键因素。我国大部分干细胞资源库的标准化建设仍处于起步阶段,面临资源合规性建设不健全、质量标准体系建设不够完善、资源收集和共享渠道不流畅以及可持续性发展规划与建设不足等多方面挑战。这些挑战不仅限制了干细胞资源库的高质量发展,而且在很大程度上阻碍了该领域的规范化进程。有鉴于此,本文深入分析干细胞资源库在推动相关领域发展中的关键作用及面临的主要挑战,阐明标准化建设的重要性和紧迫性,并进一步探讨我国干细胞资源库的发展现状及标准化建设的可行路径。通过加强合规性建设、质量管理、数据化建设和国际合作,干细胞资源库将有助于推动再生医学的健康发展,为基础研究和临床应用提供高质量的细胞资源,促进生物医药领域的持续创新。

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Progress in human stem cell-derived models for developmental and reproductive toxicology studies

Reproductive Toxicology, Год журнала: 2022, Номер 113, С. 170 - 171

Опубликована: Сен. 24, 2022

Язык: Английский

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