Cellular and Molecular Bioengineering, Год журнала: 2024, Номер 17(4), С. 243 - 261
Опубликована: Авг. 1, 2024
Язык: Английский
Journal of Ophthalmology, Год журнала: 2025, Номер 2025(1)
Опубликована: Янв. 1, 2025
Severe dry eye disease (DED) is an inflammatory condition characterized by a lack of sufficient moisture or lubrication on the surface eye, significantly impacting quality life and visual function. Since detrimental immune response crucially responsible for development aggravation DED, therapeutic agents which modulate phenotype function eye-infiltrated cells could be used treatment severe DED. Due to their potent immunomodulatory properties, mesenchymal stem (MSCs) represent potentially new remedies diseases. The majority MSC-sourced bioactive factors are contained within MSC-derived exosomes (MSC-Exos), nano-sized extracellular vesicles which, due nanosize dimension lipid envelope, easily pass all biological barriers in body deliver cargo directly into target cells. MSC-Exos contain variety proteins (growth factors, immunoregulatory molecules, cytokines, chemokines) lipids, microRNAs (miRNAs) affect viability, proliferation, phenotype, Accordingly, may progression diseases, including Therefore, this review article, we summarized current knowledge regarding molecular cellular mechanisms were trophic, anti-inflammatory, immunoregulatory, regenerative properties For purpose, extensive literature was carried out February 2024 across several databases (Medline, Embase, Google Scholar), from 2000 present. Eligible studies delineated MSC-Exos-based modulation cell-driven inflammation findings analyzed review. Results obtained these demonstrated beneficial effects paving way future clinical use ophthalmology. Trial Registration: ClinicalTrials.gov identifier: NCT04213248, NCT06475027, NCT06543667, NCT05738629.
Язык: Английский
Процитировано
1
Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 13
Опубликована: Март 10, 2025
Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs), especially, exosomes are considered to have diverse therapeutic effects for various significant diseases. MSC-derived (MSCex) offer substantial advantages over MSCs due their long-term preservation, stability, absence of nuclei and fewer adverse such as infusion toxicity, thereby paving the way towards regenerative medicine cell-free therapeutics. These harbor several cellular contents DNA, RNA, lipids, metabolites, proteins, facilitating drug delivery intercellular communication. MSCex ability immunomodulate trigger anti-inflammatory process hence, playing a key role in alleviating inflammation enhancing tissue regeneration. In this review, we addressed underlying immunomodulatory pathways. Moreover, discussed recent updates on treating specific inflammatory diseases, including arthritis, bowel disease, eye respiratory diseases asthma acute distress syndrome (ARDS), well neurodegenerative cardiac Finally, highlighted challenges using successful tool future perspectives.
Язык: Английский
Процитировано
1
Molecular Neurobiology, Год журнала: 2025, Номер unknown
Опубликована: Май 10, 2025
Язык: Английский
Процитировано
1
Heliyon, Год журнала: 2024, Номер 10(20), С. e39251 - e39251
Опубликована: Окт. 1, 2024
Язык: Английский
Процитировано
4
European Journal of Pharmacology, Год журнала: 2025, Номер 992, С. 177349 - 177349
Опубликована: Фев. 5, 2025
Spinal cord injury (SCI) is a devastating event for the central nervous system (CNS), often resulting in loss of sensory and motor functions. It profoundly affects both physiological psychological well-being patients, reducing their quality life while also imposing significant economic pressure on families healthcare system. Due to complex pathophysiology SCI, effective treatments promoting recovery remain scarce. Mesenchymal stem cell-derived exosomes (MSC-Exos) offer advantages such as low immunogenicity, good biocompatibility, ability cross blood-spinal barrier (BSCB). In preclinical studies, they have progressively shown efficacy SCI repair functional recovery. However, yield insufficient targeting MSC-Exos limit therapeutic efficacy. Currently, genetic engineering other preprocessing techniques are being employed optimize properties exosomes, thereby enhancing potential. Therefore, this paper provides an overview biogenesis exosomes. summarizes current approaches optimizing exosome performance. Additionally, it details mechanisms through which optimized provide neuroprotection explores potential combined involving hydrogels.
Язык: Английский
Процитировано
0
Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 81 - 104
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Clinical and Experimental Dental Research, Год журнала: 2025, Номер 11(1)
Опубликована: Фев. 1, 2025
ABSTRACT Objective Cell secretomes represent a promising strategy for periodontal and bone regeneration. The objective of this study was to characterize the secretome human gingival fibroblasts (GF) from periodontally diseased tissues (GF‐perio) using proteomics. Materials Methods Conditioned media GF‐perio periodontitis patients ( n = 6, 48‐h serum‐free culture) were subjected liquid chromatography with tandem mass spectrometry. Global profiles, differentially expressed proteins (DEPs), functional/gene‐set enrichment (FEA) analyzed bioinformatics. Selected regeneration‐related additionally measured multiplex immunoassay. GF healthy subjects used as reference. Results Overall, 1833 detected in secretomes, including several growth factors, cytokines, chemokines, extracellular matrix important wound healing Key bone‐related cytokines (FGF2, MCP1, GPNMB, MMP2, IL6, IL8) confirmed by an Compared reference group, 127 exclusive 73 DEPs p < 0.05) identified group. FEA revealed significant “exosome” “cytoplasm” related cellular components secretomes. Conclusion may hold therapeutic potential, regeneration, especially those exosome functions.
Язык: Английский
Процитировано
0
Arabian Journal of Chemistry, Год журнала: 2025, Номер 0, С. 1 - 10
Опубликована: Март 11, 2025
Язык: Английский
Процитировано
0
Regenerative Therapy, Год журнала: 2025, Номер 29, С. 455 - 465
Опубликована: Апрель 23, 2025
Язык: Английский
Процитировано
0
Journal of the Egyptian National Cancer Institute, Год журнала: 2025, Номер 37(1)
Опубликована: Май 1, 2025
Extracellular vesicles (EVs) have emerged as key cell-to-cell communication mediators and play significant roles in both physiological pathological processes. In EVs, exosomes represent a distinct subpopulation of EVs that been found to be involved cancer initiation therapeutic resistance. Exosomes transfer diverse spectrum molecular cargos effects on the tumor microenvironment (TME), thereby enabling initiation, metastasis, recently interest therapy due their role important treatment The exosomal content-proteins, miRNAs, lncRNAs-allows perform functions including drug efflux detoxification, cell death pathway modulation, induction epithelial-to-mesenchymal transition (EMT), suppression immune system. addition facilitating stromal interactions, cause extracellular matrix remodeling induce heterogeneity, making it more difficult respond therapy. This review covers intricate resistance with regard biogenesis, content, functional impact TME. Along this, we also discuss new strategies overcome exosome-mediated utilizing exosome inhibitors, designed therapy, combination conventional therapies. While hold promise prediction diagnosis through biomarker function, heterogeneous origins cryptic make target interventions. emphasizes research pathways is urgently required develop can improve outcomes.
Язык: Английский
Процитировано
0