Chitosan Nanoparticles as a Strategy for enhancing the Tropane Alkaloid Compounds Production and h6h Gene Expression in Hyoscyamus reticulatus Hairy Roots

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 14, 2025

Background: Hyoscyamus reticulatus L. is a rich source of tropane alkaloids (TA) which widely used in medicine. In this study, hairy roots were obtained from two-week cotyledon explants H. by using the A7 strain Agrobacterium rhizogenes. The effects different concentrations chitosan nanoparticles (CNP) (0, 10, 30, 50 and 100 mg L⁻¹) as signaling molecule with two exposure times (24 48 h) on growth rate, antioxidant enzymes activity, phenol content, TA contents hyoscyamine-6-beta-hydroxylase (h6h) gene expression levels investigated. After extraction roots, High-performance liquid chromatography (HPLC) was to measure amount hyoscyamine scopolamine. Results: results showed that highest (1300.3 μg g⁻¹ FW) scopolamine (918.06 accumulated treated L⁻¹ CNP at 48h 10 24h, respectively. present effect on h6h also investigated semi-quantitative RT-PCR. observed at concentration 24h lowest control sample. Conclusions: Based these results, it concluded CNP can be an effective stimulant for production plant secondary metabolites, including TA, probably due stimulation TA.

Язык: Английский

---

Plant growth regulator studies and emerging biotechnological approaches in Artemisia annua L.: A comprehensive overview

South African Journal of Botany, Год журнала: 2025, Номер 181, С. 181 - 222

Опубликована: Апрель 21, 2025

Язык: Английский

---

Enhanced and predictive modelling of direct shoot regeneration of Evolvulus alsinoides (L.) using ANN machine learning model and Genetic stability studies

Current Plant Biology, Год журнала: 2024, Номер unknown, С. 100423 - 100423

Опубликована: Ноя. 1, 2024

Язык: Английский

---

Cutting-Edge Strategies to Enhance Bioactive Compound Production in Plants: Potential Value of Integration of Elicitation, Metabolic Engineering, and Green Nanotechnology

Agronomy, Год журнала: 2024, Номер 14(12), С. 2822 - 2822

Опубликована: Ноя. 27, 2024

The increasing demand for bioactive compounds from plants has spurred the exploration of innovative strategies to enhance their production. This review focuses on three advanced methodologies: elicitation, metabolic engineering, and using biosynthesized nanoparticles. Elicitation, through application abiotic biotic stressors, stimulates plant defense mechanisms, significantly boosts secondary metabolite Metabolic engineering further refines this process by genetically modifying key pathways redirect resources towards synthesis valuable compounds. Additionally, metallic oxide nanoparticles offer a sustainable method growth These act not only as elicitors, but also catalyze processes improve efficiency compound synthesis. Despite significant advancements, challenges remain in optimizing these approaches large-scale agricultural application, particularly understanding precise interactions between systems. integration holds great potential agriculture development high-value phytochemicals. Future research should focus unraveling complex mechanisms behind refining methods commercial use, ensuring environmental economic sustainability.

Язык: Английский

---

Carbon Dot Unravels Accumulation of Triterpenoid in Evolvulus alsinoides Hairy Roots Culture by Stimulating Growth, Redox Reactions and ANN Machine Learning Model Prediction of Metabolic Stress Response

Plant Physiology and Biochemistry, Год журнала: 2024, Номер 216, С. 109142 - 109142

Опубликована: Сен. 27, 2024

Язык: Английский

---

The Untapped Potential of Hairy Root Cultures and Their Multiple Applications

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12682 - 12682

Опубликована: Ноя. 26, 2024

Plants are rich sources of specialized metabolites, such as alkaloids, terpenes, phenolic acids, flavonoids, coumarins, and volatile oils, which provide various health benefits including anticancer, anti-inflammatory, antiaging, skin-altering, anti-diabetic properties. However, challenges low inconsistent yields, environment geographic factors, species-specific production some metabolites limit the supply raw plant material for food, cosmetic, or pharmaceutical industries. Therefore, biotechnological approaches using in vitro systems offer an appealing alternative biologically active metabolites. Among these, hairy root cultures induced by Rhizobium rhizogenes have firmed up their position “green cell factories” due to genotypic biosynthetic stability. Hairy roots valuable platforms producing high-value phytomolecules at a cost, amenable pathway engineering, can be scaled bioreactors, making them attractive commercialization. This review explores potential biosynthesis focusing on biotechnology tools enhance production. Aspects morphological peculiarities roots, diversity bioreactors design, process intensification technologies maximizing capacity, well examples patented plant-derived (green-labeled) products produced through cultivation lab industrial scales, addressed discussed.

Язык: Английский

---

Induction of hairy roots a promising and useful biotechnological tool in tobacco (Nicotiana tabacum L.) and bitter apple (Citrullus colocynthis L.) plants using Rhizobium rhizogenes

South African Journal of Botany, Год журнала: 2024, Номер 177, С. 421 - 428

Опубликована: Дек. 24, 2024

Язык: Английский

---