Production of short-chain fatty acids (SCFAs) as chemicals or substrates for microbes to obtain biochemicals

Biotechnology for Biofuels and Bioproducts, Год журнала: 2023, Номер 16(1)

Опубликована: Июнь 3, 2023

Carboxylic acids have become interesting platform molecules in the last years due to their versatility act as carbon sources for different microorganisms or precursors chemical industry. Among carboxylic acids, short-chain fatty (SCFAs) such acetic, propionic, butyric, valeric, and caproic can be biotechnologically produced an anaerobic fermentation process from lignocellulose other organic wastes of agricultural, industrial, municipal origin. The biosynthesis SCFAs is advantageous compared synthesis, since latter relies on fossil-derived raw materials, expensive toxic catalysts harsh conditions. This review article gives overview complex waste products. Different applications are explored how these considered a source bioproducts, aiming at development circular economy. use requires adequate concentration separation processes that also addressed this review. Various bacteria oleaginous yeasts efficiently SCFA mixtures derived fermentation, attribute exploited microbial electrolytic cells produce biopolymers oils polyhydroxyalkanoates. Promising technologies conversion into bioproducts outlined with recent examples, highlighting future bioeconomy.

Язык: Английский

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Microbial Lipid Based Biorefinery Concepts: A Review of Status and Prospects

Foods, Год журнала: 2023, Номер 12(10), С. 2074 - 2074

Опубликована: Май 22, 2023

The use of lignocellulosic biomass as a raw material for the production lipids has gained increasing attention, especially in recent years when food biofuels become current technology. Thus, competition materials both uses brought need to create technological alternatives reduce this that could generate reduction volume offered and consequent commercial increase value food. Furthermore, microbial oils been studied many industrial branches, from generation renewable energy obtainment several value-added products pharmaceutical industries. review provides an overview feasibility challenges observed through biorefinery. Topics covered include biorefining technology, oil market, oily microorganisms, mechanisms involved lipid-producing metabolism, strain development, processes, lipids, technical drawbacks, lipid recovery.

Язык: Английский

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Co-valorization of crude glycerol and low-cost substrates via oleaginous yeasts to micro-biodiesel: Status and outlook

Renewable and Sustainable Energy Reviews, Год журнала: 2023, Номер 180, С. 113303 - 113303

Опубликована: Апрель 24, 2023

Язык: Английский

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New trends in microbial lipid-based biorefinery for fermentative bioenergy production from lignocellulosic biomass

Biofuel Research Journal, Год журнала: 2024, Номер 11(01), С. 2040 - 2064

Опубликована: Март 1, 2024

Using oleaginous microbial lipid-based biorefinery from lignocellulosic biomass (LCB) to produce fermentative bioenergy (i.e., biodiesel) represents an innovative second-generation fuel production technology. These lipids are predominantly intracellular triglycerides that accumulate through the metabolism of sugars in fermentation following pretreatment and enzymatic hydrolysis LCB. This review investigates recent advances lipid LCB, focusing on factors influencing lead producers, different methods physical, chemical, biological, combined pretreatment), approaches, novel bioprocessing strategies microbes-specific model specific), engineering techniques microbes genetic metabolic alterations). The study demonstrates yeasts can synthesize significantly higher quantities when incorporated into system, known as separated production, various methods. Interestingly, CRISPR is found be most suitable way genetically metabolically for increased synthesis. also explores economically viable addressing associated challenges, outlines future directions, including comprehensive techno-economic life cycle assessments. offers invaluable insights highlighting potential significant technological environmental enhancements ongoing research development efforts.

Язык: Английский

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Valorization of durian peel as a carbon feedstock for a sustainable production of heterogeneous base catalyst, single cell oil and yeast-based biodiesel

Carbon Resources Conversion, Год журнала: 2024, Номер 7(3), С. 100224 - 100224

Опубликована: Янв. 20, 2024

The present study reports a successful attempt to produce single cell oil (SCO), heterogeneous base catalyst and yeast-based biodiesel from durian peel as promising carbon feedstock by means of the waste-to-energy concept. For this purpose, first, (DP) was hydrolyzed dilute sulfuric acid obtain xylose-rich DP hydrolysate (XDPH) post-hydrolysis solid residue (DPS). Candida viswanathii PSY8, newly isolated oleaginous yeast, showed high SCO accumulation (5.1±0.1 g/L) content (35.3 ± 0.13%) on undetoxified XDPH medium. A novel (DPS-K) prepared DPS wet impregnation technique with KOH, exhibited considerable catalytic activity convert SCO-rich yeast C. PSY8 into (FAME) via direct transesterification maximum FAME yield 94.3% under optimal conditions (6 wt% catalyst, 10:1 methanol ratio, 75 °C, 2 h). Moreover, most properties obtained profiles were correlated well standards limit Thai, ASTM D6751 EN 14214. Additionally, energy output produced about 37.5 MJ/kg estimated. Thus, finding demonstrated favorable strategy for sustainable eco-friendly production new generation biodiesel.

Язык: Английский

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A review of lipid accumulation by oleaginous yeasts: Culture mode

The Science of The Total Environment, Год журнала: 2024, Номер 919, С. 170385 - 170385

Опубликована: Фев. 2, 2024

Язык: Английский

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Response surface methodology and repeated-batch fermentation strategies for enhancing lipid production from marine oleaginous Candida parapsilosis Y19 using orange peel waste

Microbial Cell Factories, Год журнала: 2025, Номер 24(1)

Опубликована: Янв. 10, 2025

Abstract Oleaginous yeasts are considered promising sources for lipid production due to their ability accumulate high levels of lipids under appropriate growth conditions. The current study aimed isolate and identify oleaginous having superior quantities lipids; enhancing using response surface methodology repeated-batch fermentation. Results revealed that, twenty marine were isolated, the most potent producer was Candida parapsilosis Y19 according qualitative screening test Nile-red dye. Orange peels used as substrate where C. produced 1.14 g/l at 23.0% in batch To enhance production, statistical optimization Taguchi design through Response carried out. Total increased 2.46 content 30.7% optimal conditions of: orange peel 75 g/l, peptone 7 yeast extract 5 inoculum size 2% ( v/v ), pH incubation period 6 d. Furthermore, fermentation enhanced total 4.19 folds (4.78 g/l) compared culture (before optimization). Also, 1.7 (39.1%) Fatty acid profile includes unsaturated fatty acids (USFAs) 74.8% saturated (SFAs) 25.1%. Additionally, fermentation, major oleic 45.0%; followed by linoleic 26.0%. In conclusion, is a strain production. both optimizations RSM efficient methods from Y19.

Язык: Английский

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Production of biofuels from C₁‐gases with Clostridium and related bacteria—Recent advances

Microbial Biotechnology, Год журнала: 2023, Номер 16(4), С. 726 - 741

Опубликована: Янв. 20, 2023

Clostridium spp. are suitable for the bioconversion of C1 -gases (e.g., CO2 , CO and syngas) into different bioproducts. These products can be used as biofuels reviewed here, focusing on ethanol, butanol hexanol, mainly. The production higher alcohols hexanol) has hardly been reviewed. Parameters affecting optimization process bioreactor performance addressed well pathways involved in these bioconversions. New aspects, such mixotrophy sugar versus gas fermentation, also In addition, Clostridia produce from integration Wood-Ljungdahl pathway reverse ß-oxidation pathway, which not yet comprehensively latter process, acetogen uses reducing power CO/syngas to reduce C4 or C6 fatty acids, previously produced by a chain elongating microorganism (commonly kluyveri), corresponding bioalcohol.

Язык: Английский

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High-throughput screening of non-conventional yeasts for conversion of organic waste to microbial oils via carboxylate platform

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июнь 20, 2024

Abstract Converting waste into high-value products promotes sustainability by reducing and creating new revenue streams. This study investigates the potential of diverse yeasts for microbial oil production utilizing short-chain fatty acids (SCFAs) that can be produced from organic focuses on identifying strains with best SCFA utilisation, tolerance lipid production. A collection 1434 yeast was cultivated SCFAs as sole carbon source. Eleven emerged candidates promising growth rates high accumulation. Subsequent fermentation experiments in liquid SCFA-rich media, which focused optimizing accumulation adjusting to nitrogen (C/N) ratio, showed an increase content at a C/N ratio 200:1, but concurrent reduction biomass. Two were characterized their superior ability produce lipids compared reference strain Yarrowia lipolytica CECT124: Y. EXF-17398 Pichia manshurica EXF-7849. Characterization these two indicated they exhibit biotechnologically relevant balance between maximizing yield maintaining concentrations. These results emphasize using sustainable feedstock oleochemical production, offering dual benefit valorisation

Язык: Английский

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Poly-Unsaturated Fatty Acids (PUFAs) from Cunninghamella elegans Grown on Glycerol Induce Cell Death and Increase Intracellular Reactive Oxygen Species

Journal of Fungi, Год журнала: 2024, Номер 10(2), С. 130 - 130

Опубликована: Фев. 4, 2024

Cunninghamella elegans NRRL-1393 is an oleaginous fungus able to synthesize and accumulate unsaturated fatty acids, amongst which the bioactive gamma-linolenic acid (GLA) has potential anti-cancer activities. C. was cultured in shake-flask nitrogen-limited media with either glycerol or glucose (both at ≈60 g/L) employed as sole substrate. The assimilation rate of both substrates similar, total biomass production reached 13.0–13.5 g/L, c. 350 h after inoculation (for instances, 27–29 g/L substrate were consumed). Lipid slightly higher on glycerol-based media, compared growth (≈8.4 vs. ≈7.0 g/L). Lipids from grown glycerol, containing 9.5% w/w GLA, transformed into lithium salts (FALS), their effects assessed human normal cancerous cell lines. FALS exhibited cytotoxic within a 48 interval IC50 about 60 μg/mL. Additionally, suppression migration shown, significant elevation oxidative stress levels, induction death. Elementary differences between cancer cells not indicating generic mode action; however, level augmentation may increase susceptibility anticancer drugs, improving chemotherapy effectiveness.

Язык: Английский

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