DNA metabarcoding reveals a diverse, omnivorous diet of Arctic amphipods during the polar night, with jellyfish and fish as major prey

Frontiers in Marine Science, Год журнала: 2024, Номер 11

Опубликована: Фев. 14, 2024

Introduction Currently, Arctic marine ecosystems are witnessing the most rapid physical changes worldwide, leading to shifts in pelagic and benthic communities food web structure, concomitant with introduction of boreal species. Gelatinous zooplankton or jellyfish represent one particular group which several species prone undergo significant poleward range expansions population increases course ongoing changes. Historically, were considered a trophic dead-end, but an increasing number studies using modern tools have highlighted their role as major prey items webs. In this study, we aimed verify other metazoans sources polar night web, when resources limited. Methods We identified diet different bentho-pelagic amphipod Atlantifying Kongsfjorden (West Svalbard) during night. regularly sampled lysianassoid gammarid amphipods baited traps hand nets over period month spectrum by applying DNA metabarcoding (COI) stomach contents. Results demonstrate that all investigated omnivorous. Fish including cod snailfish, likely shape carrion, played important scavengers Orchomenella minuta Anonyx sarsi . Predation potential scavenging on contributed four species, particularly for Gammarus setosus G. oceanicus , evidenced high read abundances frequencies occurrence. Besides jellyfish, crustaceans macroalgae components two Discussion The diverse community present is clearly being utilized source, either through feeding jelly-falls, albeit extent local community. These findings provide novel insights into first evidence natural (non-experimental) jelly-falls.

Язык: Английский

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DNA metabarcoding reveals the importance of gelatinous zooplankton in the diet ofPandalus borealis, a keystone species in the Arctic

Molecular Ecology, Год журнала: 2021, Номер 31(5), С. 1562 - 1576

Опубликована: Дек. 22, 2021

Information about the dietary composition of a species is crucial to understanding their position and role in food web. Increasingly, molecular approaches such as DNA metabarcoding are used studying trophic relationships, not least because they may alleviate problems low taxonomic resolution or underestimation digestible taxa diet. Here, we with universal primers for cytochrome c oxidase I (COI) study diet northern shrimp (Pandalus borealis), an Arctic keystone large socio-economic importance. Across locations, jellyfish chaetognaths were most important components P. borealis, jointly accounting 40%-60% total read abundance. This importance gelatinous zooplankton contrasts sharply published results based on stomach content analysis. At same time, differed between fjord shelf pointing different webs supporting borealis these two systems. Our underlines potential provide new insights into marine invertebrates that difficult obtain traditional methods, calls revision key extension, webs.

Язык: Английский

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Searchin' for Urchins: Utilising Museum Collections and Citizen Science to Assess Species on the Move in the Genus Tripneustes

Journal of Biogeography, Год журнала: 2025, Номер unknown

Опубликована: Янв. 24, 2025

ABSTRACT Aim With the global redistribution of species due to warming, accurately quantifying distributions is critical understanding patterns in biodiversity and range shift trajectories. The genus Tripneustes comprises globally important sea urchin taxa that graze seagrass macroalgae have potential transform ecosystems. In eastern Australia, there are two taxa, tropical T. g. gratilla subtropical‐temperate australiae . temperate distribution was considered be a climate‐driven extension until recent taxonomic clarification. We quantified present future these warming hot spot. Location Eastern Australia Tasman Sea, including Lord Howe Island, Norfolk north New Zealand. Taxon (Echinodermata: Echinoidea: Toxopneustidae). Methods reassessed specimen identifications across four museum collections synthesise data citizen science observations, determining biogeographic realised thermal niches Habitat suitability models were used determine current suitable habitat predict distributional change. Results Whilst has wide tropical‐temperate region from Papua Guinea (9°56′2.4″ S) Jervis Bay (35°7′12″ broad niche (16.3°C–29.97°C), (Byron Bay, 28°37′0.12″ S, Narooma, 36°15′0″ narrower (15.3°C–26°C). densities highest subtropical ecoregions, where co‐occur. modelling indicated narrow‐range specialist will likely undergo poleward by 2100. contrast, generalist appears capable occupying broader conditions. Main Conclusions Despite their similar ecological roles, congeneric contrasting niches, with distinct implications for as ocean warms.

Язык: Английский

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Performance of DNA Metabarcoding vs. Morphological Methods for Assessing Intertidal Turf and Foliose Algae Diversity

Molecular Ecology Resources, Год журнала: 2025, Номер unknown

Опубликована: Апрель 24, 2025

ABSTRACT Large biogeographical shifts in marine communities are taking place response to climate change and biological invasions yet we still lack a full understanding of their diversity distribution. An important example this is turf foliose algae that key coastal primary producers several regions expanding into new environments. Traditionally, monitoring involves species identification based on morphological traits, which challenging due reduced dimensions highly variable morphology. Molecular methods promise revolutionise field, but effectiveness detecting has be tested. Here, evaluate the performance DNA metabarcoding (COI rbc L markers) (in situ photoquadrat) describe intertidal along Portuguese coast. Both molecular markers detected more taxa than showed greater discrimination between regions, matching our knowledge geographical climatic patterns for region. In sum, multi‐marker approach was efficient morphology‐based characterising coast, differentiating morphologically similar species, unicellular organisms. However, certain were identified by photoquadrat approaches not through metabarcoding, partly reference barcodes or taxonomic resolution. Metabarcoding emerges as valuable tool these communities, particularly long‐term programmes requiring accuracy, speed, reproducibility.

Язык: Английский

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Trophic niches reflect compositional differences in microbiota among Caribbean sea urchins

PeerJ, Год журнала: 2021, Номер 9, С. e12084 - e12084

Опубликована: Авг. 31, 2021

Sea urchins play a critical role in marine ecosystems, as they actively participate maintaining the balance between coral and algae. We performed first in-depth survey of microbiota associated with four free-living populations Caribbean sea urchins: Lytechinus variegatus, Echinometra lucunter, Tripneustes ventricosus, Diadema antillarum. compared influence collection site, echinoid species trophic niche to composition microbiota. This dataset provides comprehensive overview date, bacterial communities their ecological relevance natural environments. A total sixty-samples, including surrounding reef water seagrass leaves underwent 16S rRNA gene sequencing (V4 region) high-quality reads were analyzed standard bioinformatic approaches. While dominated by Cyanobacteria such Prochlorococcus Rivularia respectively, gut samples had dominant Bacteroidetes, Proteobacteria Fusobacteria. Propionigenium was across all species' guts, revealing host-associated likely responsive digestive process animals. Beta-diversity analyses showed significant differences community among three sites, animal species, niches. Alpha diversity significantly higher L. variegatus other species. also displayed an increased abundance Planctomycetes Cyanobacterial OTUs. The this herbivorous reflected similarities microfilm found on Thalassia testudinum leaves; very abundant its main food resource. results study elaborate microbial ecology important echinoids, confirming that selection is trophic-niche dependent.

Язык: Английский

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Isotopic niche shift in the sea urchins Echinometra lucunter and E. viridis after massive arrivals of Sargassum in the Mexican Caribbean

Regional Studies in Marine Science, Год журнала: 2023, Номер 65, С. 103064 - 103064

Опубликована: Июнь 26, 2023

Язык: Английский

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Differences of Macroalgal Consumption by Eight Herbivorous Coral Reef Fishes From the Xisha Islands, China

Frontiers in Marine Science, Год журнала: 2022, Номер 9

Опубликована: Апрель 12, 2022

Herbivorous fishes play an important role in preventing the overabundance of macroalgae on coral reefs. Understanding feeding selectivity and consumption by herbivorous can be challenging studies their ecological preservation recovery Coral reef decline, macroalgal overgrowth overfishing are clearly visible Xisha Islands, China. However, there seldom behaviors this area. We used microscopy, 18S rRNA high-throughput sequencing, stable isotope analyses to comprehensively examine diet eight fish species common including one parrotfish, two chub, unicorn fish, three rabbitfish. Based microscopic examination, Siganus argenteus fed highest number (five species), followed Naso unicornis (four species). Kyphosus cinerascens , K. vaigiensis N. S. punctatissimus entire thallus, indicating greater importance compared with that only consume algal fronds. According sequencing intestinal contents, cluster analysis revealed consumed composition from always grouped together based species, but low similarity. removed diversity as well greatest quantity macroalgae. Calotomus carolinus red alga Pneophyllum conicum which is widely distributed Indo-Pacific reefs overgrow kill live corals. was found occupy lowest trophic position analysis. Multi-technique showed a high potential macroalgae, suggesting they key browsers should receive priority protection Islands. A diverse fauna also very These results not demonstrated various functions different removal, provided insights into management South China Sea.

Язык: Английский

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High-Throughput Sequencing Analysis Revealed a Preference for Animal-Based Food in Purple Sea Urchins

Biology, Год журнала: 2024, Номер 13(8), С. 623 - 623

Опубликована: Авг. 15, 2024

Sea urchins play an important role in marine ecosystems. Owing to limitations previous research methods, there has been insufficient understanding of the food sources and ecological functional value purple sea urchins, leading considerable controversy regarding their positioning. We focused on Daya Bay as area, utilizing stable isotope technology high-throughput sequencing 16S rDNA 18S analyze potential stone algae areas. The results showed that δ13C range area is −11.42~−8.17‰, δ15N 9.15~10.31‰. However, algal −13.97~−12.44‰, 8.75~10.14‰. There was a significant difference between two areas (p < 0.05), but no > 0.05). main source for both sediment. revealed that, highest proportion urchin gut Molluska (57.37%). In Arthropoda (76.71%). Bacteroidetes dominant group (28.87%), whereas, Proteobacteria (37.83%). Diversity detection number microbes eukaryotes sediment, organic nutritional greater richness microbiota greater. These indicated are likely omnivores where they occur impacts growth development. this study provide theoretical basis restoration wild resources selection restocking release.

Язык: Английский

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Recent molecular techniques to strengthen ecological studies in echinoderms

Revista de Biología Tropical, Год журнала: 2024, Номер 72(S1), С. e58880 - e58880

Опубликована: Март 1, 2024

Introduction: Echinoderms, an integral component of marine ecosystems worldwide, have captivated scientific interest for centuries. Despite this longstanding attention, comprehending key facets such as trophic relationships, diet composition, and host-microbiota relationships still represents a challenge using traditional techniques. Recent years, however, witnessed transformative shift, thanks to the emergence advanced molecular techniques, offering new approaches strengthen ecological studies in echinoderms. Objective: Explore how recent advancements tools impacted research on Specifically, we aim investigate potential these shed light interactions, characterization gut microbial communities organisms. Methods: Available literature was used clarify novel techniques can improve studies. The focus is diet, microbiota. Results: Traditionally, stomach contents compound microscopy provided idea ingested material; nevertheless, sometimes simple magnified visualization dietary content does not allow exhaustive identification entire food spectrum, it limited due rapid digestion maceration items within echinoderm’s digestive tract. use DNA-metabarcoding, targeting specific DNA regions, mitochondrial COI gene, has allowed us enhance accuracy precision by enabling prey down species or even genetic variant level, providing valuable insights into preferences. Another approach stable isotopes, particularly carbon nitrogen, which provide powerful tool trace origin flow nutrients through webs. By analyzing isotopic signatures muscular tissues items, discern sources their primary gain position ecosystem. Lastly, third technique elucidate prokaryotic community 16S rRNA sequencing. This method allows explore composition dynamics tract communities. Conclusions: promising era echinoderms, where advances enabled unprecedented level detail, resolving challenges opening avenues investigation

Язык: Английский

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Effects of temperature and size class on the gut digesta microbiota of the sea urchin Tripneustes ventricosus

PeerJ, Год журнала: 2024, Номер 12, С. e18298 - e18298

Опубликована: Ноя. 28, 2024

Background Understanding the dynamics of gut microbiota in sea urchins is crucial for comprehending ecological balance marine ecosystems. The plays a vital role nutrient metabolism, immune system modulation, and pathogen protection. microbial composition naturally occurring urchin Tripneustes ventricosus have yet to be thoroughly investigated. We hypothesized T. Caribbean, varies across life stages seasons. Methods Thirty-six large individuals six small (42 animals) were collected from shallow waters on northeastern coast Puerto Rico February August 2019. fecal pellet’s was characterized by sequencing V4 region 16S rRNA gene. Results found significant differences pellet between seasons stages. Phylum Bacteroidota had greater relative abundance August, while Firmicutes more dominant February. Propionigenium Roseimarinus Candidatus Hepatoplasma, Kistimonas Differences digest not urchins, but displayed slightly higher diversity dominance Proteobacteria, exhibited Fusobacteria Desulfobacterota. However, genera Ferrominas counts significantly lower individuals. Discussion This first report this species Caribbean adds our comprehension white collection periods size classes, highlighting dynamic nature microbiota.

Язык: Английский

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