Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Ноя. 28, 2024
The
study
focuses
on
the
in
silico
analysis
of
cysteine-rich
secretory
proteins
and
PR1-like
(CAP)
genes
finger
lime
(Citrus
australasica),
a
citrus
species
known
for
its
tolerance
to
Huanglongbing
(HLB).
We
identified
several
genes,
all
belonging
CRISP
family
within
CAP
superfamily.
Of
them,
CaCAP2
transcript
levels
increased
by
over
300-fold
compared
'Valencia'
sweet
orange
upon
infection
with
'Candidatus
Liberibacter
asiaticus'
(CaLas).
Localization
studies
using
an
EGFP
fusion
showed
that
CAP2
protein
is
predominantly
located
nucleus,
extracellular
plasma
membrane.
also
examined
response
cold,
drought
stress,
salicylic
acid
application.
Despite
environmental
stress
causing
apparent
damage,
seem
play
significant
role
managing
both
biotic
abiotic
stresses.
Analysis
gene
promoters
from
revealed
95.33%
sequence
identity,
variations
transcription
factor-binding
sites
cis-acting
elements
such
as
Stress
Response
Element
(STRE:
AGGGG),
which
might
influence
differential
expression
between
two
species.
Additionally,
expressing
lime-derived
transgenic
Nicotiana
tabacum
induced
strong
defense
against
Pseudomonas
syringae
pv.
Tabaci.,
underscoring
gene's
crucial
plant
mechanisms
bacterial
pathogens.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(1), С. 638 - 638
Опубликована: Янв. 4, 2024
Chitinases,
which
catalyze
the
hydrolysis
of
chitin,
primary
components
fungal
cell
walls,
play
key
roles
in
defense
responses,
symbiotic
associations,
plant
growth,
and
stress
tolerance.
In
this
study,
23
chitinase
genes
were
identified
watermelon
(Citrullus
lanatus
[Thunb.])
classified
into
five
classes
through
homology
search
phylogenetic
analysis.
The
with
similar
exon-intron
structures
conserved
domains
clustered
same
class.
putative
cis-elements
involved
responses
to
phytohormone,
stress,
development
their
promoter
regions.
A
tissue-specific
expression
analysis
showed
that
ClChi
primarily
expressed
roots
(52.17%),
leaves
(26.09%),
flowers
(34.78%).
Moreover,
qRT-PCR
results
indicate
ClChis
multifaceted
interaction
between
plant/environment.
More
members
induced
by
Race
2
Fusarium
oxysporum
f.
sp.
niveum,
eight
at
higher
levels
on
seventh
day
after
inoculation
Races
1
2,
suggesting
these
a
role
resistance
wilt.
Collectively,
improve
knowledge
gene
family
species
help
elucidate
played
chitinases
various
stresses.
Plants,
Год журнала:
2024,
Номер
13(15), С. 2022 - 2022
Опубликована: Июль 23, 2024
Climate
change
presents
numerous
challenges
for
agriculture,
including
frequent
events
of
plant
abiotic
stresses
such
as
elevated
temperatures
that
lead
to
heat
stress
(HS).
As
the
primary
driving
factor
climate
change,
HS
threatens
global
food
security
and
biodiversity.
In
recent
years,
have
negatively
impacted
physiology,
reducing
plant's
ability
maintain
disease
resistance
resulting
in
lower
crop
yields.
Plants
must
adapt
their
priorities
toward
defense
mechanisms
tolerate
challenging
environments.
Furthermore,
selective
breeding
long-term
domestication
higher
yields
made
varieties
vulnerable
multiple
stressors,
making
them
more
susceptible
events.
Studies
on
predict
concurrent
biotic
will
become
severe
future,
potentially
occurring
simultaneously
or
sequentially.
While
most
studies
focused
singular
effects
systems
examine
how
plants
respond
specific
stresses,
simultaneous
occurrence
pose
a
growing
threat
agricultural
productivity.
Few
explored
interactions
between
plant-biotic
interactions.
Here,
we
aim
shed
light
physiological
molecular
(bacteria,
fungi,
oomycetes,
nematodes,
insect
pests,
pollinators,
weedy
species,
parasitic
plants),
well
combined
impact
growth
We
also
advances
designing
developing
various
strategies
address
multi-stress
scenarios
related
factors.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(12), С. 6463 - 6463
Опубликована: Июнь 12, 2024
Plant
resistance
against
biotic
stressors
is
significantly
influenced
by
pathogenesis-related
1
(PR1)
proteins.
This
study
examines
the
systematic
identification
and
characterization
of
PR1
family
genes
in
sugarcane
(
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Фев. 25, 2025
Reverse
transcription-quantitative
PCR
(RT-qPCR)
is
a
powerful
tool
for
quantifying
gene
expression.
However,
reference
genes
(RGs)
expression
analysis
in
peach
(Prunus
persica)
during
interactions
with
Monilinia
laxa,
major
fungal
pathogen
that
causes
brown
rot,
have
not
been
established.
In
this
study,
we
analysed
12
candidate
RGs
pathosystem
by
analysing
samples
from
to
144
HAI.
The
stability
of
the
was
evaluated
using
ΔCq
method
and
BestKeeper,
NormFinder,
geNorm
algorithms.
Our
results
identified
AKT3,
RNA
pol
II
(RPII)
SNARE
(using
geNorm),
RPII,
AKT3
TEF2
NormFinder),
RPII
BestKeeper)
MUB6
method)
as
most
stable
mRNA
normalisation
across
all
tested
samples.
algorithm
used
determine
optimal
number
suitable
required
proper
under
these
experimental
conditions,
indicating
three
were
sufficient
normalisation.
Analysis
obtained
different
algorithms
showed
RGs.
Furthermore,
confirm
validity
genes,
levels
six
interest,
involved
metabolic
pathways,
normalized
inoculated
uninoculated
fruit.
These
findings
provide
set
accurate
RT-qPCR
studies
involving
M.
laxa
interactions,
facilitating
deeper
insights
into
molecular
mechanisms
underlying
important
plant–pathogen
relationship.
