Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Ноя. 28, 2024
The
study
focuses
on
the
in
silico
analysis
of
cysteine-rich
secretory
proteins
and
PR1-like
(CAP)
genes
finger
lime
(Citrus
australasica),
a
citrus
species
known
for
its
tolerance
to
Huanglongbing
(HLB).
We
identified
several
genes,
all
belonging
CRISP
family
within
CAP
superfamily.
Of
them,
CaCAP2
transcript
levels
increased
by
over
300-fold
compared
'Valencia'
sweet
orange
upon
infection
with
'Candidatus
Liberibacter
asiaticus'
(CaLas).
Localization
studies
using
an
EGFP
fusion
showed
that
CAP2
protein
is
predominantly
located
nucleus,
extracellular
plasma
membrane.
also
examined
response
cold,
drought
stress,
salicylic
acid
application.
Despite
environmental
stress
causing
apparent
damage,
seem
play
significant
role
managing
both
biotic
abiotic
stresses.
Analysis
gene
promoters
from
revealed
95.33%
sequence
identity,
variations
transcription
factor-binding
sites
cis-acting
elements
such
as
Stress
Response
Element
(STRE:
AGGGG),
which
might
influence
differential
expression
between
two
species.
Additionally,
expressing
lime-derived
transgenic
Nicotiana
tabacum
induced
strong
defense
against
Pseudomonas
syringae
pv.
Tabaci.,
underscoring
gene's
crucial
plant
mechanisms
bacterial
pathogens.
Physiologia Plantarum,
Год журнала:
2024,
Номер
176(6)
Опубликована: Ноя. 1, 2024
Climate
changes
and
environmental
contaminants
are
daunting
challenges
that
require
an
urgent
change
from
current
agricultural
practices
to
sustainable
agriculture.
Biostimulants
natural
solutions
adhere
the
principles
of
organic
farming
believed
have
low
impacts
on
environment
human
health.
Further,
they
may
contribute
reducing
use
chemical
inputs
while
maintaining
productivity
in
adverse
environments.
generally
defined
as
formulated
substances
microorganisms
showing
benefits
for
plant
growth,
yield,
rhizosphere
function,
nutrient-use
efficiency,
quality
harvested
products,
or
abiotic
stress
tolerance.
These
biosolutions
categorized
different
subclasses.
Several
them
enriched
glycomolecules
their
oligomers.
However,
very
few
studies
considered
active
molecules
biostimulation
a
subclass
own.
Herein,
we
describe
structure
functions
complex
polysaccharides,
glycoproteins,
glycolipids
relation
defense
biostimulation.
We
also
discuss
parallels
between
sugar-enhanced
with
introduce
concept
sweet
glycostimulation.
Agronomy,
Год журнала:
2025,
Номер
15(4), С. 802 - 802
Опубликована: Март 24, 2025
Peppers
are
widely
cultivated
around
the
world,
yet
they
suffer
from
infections
caused
by
Phytophthora
capsici
fungi
throughout
year,
which
severely
impacts
their
yield.
The
β-1,3-glucanase
gene
has
been
shown
in
previous
studies
to
significantly
enhance
plants’
ability
cope
with
both
biotic
and
abiotic
stresses,
including
fungal
infections.
However,
its
function
peppers
not
reported.
In
this
study,
80
CaBG
genes
were
initially
identified,
before
being
filtered
down
54
CaBGs
peppers,
analyses
conducted
on
physicochemical
properties,
chromosomal
localization,
phylogenetic
tree
relationships,
synteny,
promoters,
expression
levels
of
BG
family.
results
indicated
that
these
located
12
chromosomes.
Phylogenetic
analysis
classified
into
three
subfamilies,
α,
β,
γ,
each
own
specific
functions,
γ
subfamily
associated
disease
resistance
peppers.
Synteny
showed
relatively
conserved
have
undergone
extensive
whole-genome
duplication
events.
Promoter
induced
plant
hormones
various
external
stressors.
Transcriptomic
RT-qPCR
revealed
CaBG7
CaBG12
activated
following
infection
P.
capsici,
two
markedly
higher
resistant
plants
compared
susceptible
ones.
Based
analysis,
we
propose
may
be
key
for
chili
This
study
provides
a
theoretical
basis
breeding
capsici-resistant
molecular
methods.
Horticulture Advances,
Год журнала:
2025,
Номер
3(1)
Опубликована: Апрель 2, 2025
Abstract
Pathogenesis-related
proteins
(PRs)
are
essential
for
plant
defense
against
both
biotic
and
abiotic
stresses.
However,
knowledge
regarding
PRs
in
pears
remains
limited.
In
this
study,
637
PRs,
classified
into
16
families,
were
identified
Pyrus
bretschneideri
.
Phylogenetic,
sequence
similarity,
secondary
structure
analyses
enabled
the
classification
of
several
with
conserved
sequences,
including
PR-11,
PR-8,
PR-15,
PR-16.
Genomic
analysis
revealed
that
expansion
most
PR
families
resulted
from
recent
whole-genome
duplication
(WGD)
events,
occurring
approximately
30–45
million
years
ago
(Mya).
Various
gene
mechanisms
have
collectively
contributed
to
proliferation
purifying
selection
indicated
by
low
Ka/Ks
ratios.
Examination
apoplastic
fluid
composition
showed
only
a
subset
was
present
apoplast.
Transcriptomic
data
infected
Erwinia
amylovora
Colletotrichum
fructicola
expression
profiles
genes
post-infection,
underscoring
complexity
regulation.
This
study
elucidates
evolutionary
expansion,
regulation,
functional
roles
disease
resistance,
highlighting
certain
as
reliable
markers
pear
resistance.
These
findings
offer
valuable
insights
future
breeding
strategies
enhance
resistance
pathogens.
Bulletin of KSAU,
Год журнала:
2025,
Номер
0(3), С. 41 - 51
Опубликована: Март 10, 2025
The
aim
of
the
study
is
to
investigate
effect
drought,
high-temperature
stress
and
their
combination
on
transcriptional
reactions
grapes
different
origins
that
determine
immune
response.
was
conducted
grape
varieties
under
simulated
conditions.
methods
are
generally
accepted.
origin
variety
affected
resistance
factors.
Cabernet
Sauvignon
equally
damaged
by
high
temperature
combined
(EL
increase
22
%).
Moldova
Kurchansky
were
drought
21
19
%,
respectively),
Morozko
31
%
45
%.
Expression
PR2,
PR3,
STS
"immune
response"
genes
consistent
with
predominant
or
temperature.
When
combined,
expression
level
these
decreased
relative
effects
individual
stresses,
while
NCED
MYC2
genes,
which
response
abiotic
stress,
increased.
Each
demonstrated
features
adaptation
combination.
It
found
conditions
temperatures
defense
against
pathogens
actively
function,
two
factors
reduces
"immune"
protection
Euro-American
(Moldova,
Kutuzovsky).
Euro-Amur
characterized
an
in
PR5
PR10
associated
stressors,
can
provide
enhanced
reactions.
Abstract
Alfalfa
(
Medicago
sativa
L.),
a
vital
leguminous
forage
crop,
faces
growth
and
distribution
constraints
due
to
abiotic
stresses,
such
as
drought.
Pathogenesis‐related
protein
1
(PR‐1)
is
major
class
of
plant
defense
proteins
involved
in
responses
environmental
stress.
However,
the
functional
roles
PR‐1
gene
family
alfalfa
under
drought
stress
remain
largely
unexplored.
Nineteen
MsPR‐1
genes
were
identified
genome
through
systematic
screening,
with
phylogenetic
clustering
dividing
these
into
three
evolutionarily
distinct
clades.
Expression
profiling
highlighted
MsPR‐1‐12
candidate
for
tolerance.
Subcellular
localization
indicated
that
localized
nucleus
membranes
cellular
membranes.
Furthermore,
reverse
transcription
quantitative
PCR(RT‐qPCR)
analysis
demonstrated
significant
upregulation
Heterologous
expression
yeast
conferred
enhanced
resistance.
These
findings
provide
novel
insights
offer
valuable
genetic
resources
development
drought‐tolerant
varieties
engineering.
