Cloning, In Silico Characterization, and Physiochemical Analysis of the Zea mays PR-1 Gene: Implications for Developing Fungi-Resistant Transgenic Plants DOI
Muhammad Saleem,

Zaher Uddin Babar,

Sumaia Saif

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 27, 2024

Abstract Pathogenesis-related protein 1 (PR-1) is a critical component of plant defense mechanisms, exhibiting significant antifungal activity. This study focused on the PR-1 from Zea mays and five other species wheat, barley, rice, oats, rye to explore their physicochemical, structural, functional characteristics. The gene was successfully isolated Z. amplified using Polymerase Chain Reaction (PCR). Following cloning into pET15b vector, expressed in BL21-DE3 (E. coli) cells. Induction with 1mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) for 4 hours at 37°C led successful expression, as confirmed by 12% SDS-PAGE. exhibited resistance against broad spectrum oomycete pathogens, demonstrating its potential developing fungi-resistant transgenic plants. revealed that proteins different shared high sequence similarity, identities ranging 65–90%. However, physicochemical properties varied based origin. cereals (maize, rice) were found be more hydrophobic compared those grasses (oats rye). Structurally, all adopted similar three-dimensional conformation, featuring central beta-barrel surrounded alpha-helices. Functional assays six activity diverse array fungal pathogens. mays, along species, shows agricultural biotechnology. By incorporating these genes crop plants, it possible enhance thereby advancing development resilient sustainable systems.

Язык: Английский

Arginine and Spermine Ameliorate Water Deficit Stress in Fenugreek (Trigonella foenum-graecum L.) by Enhancing Growth and Physio-Biochemical Processes DOI Creative Commons
Ali A. Badawy,

Wadha Kh. Alshammari,

Noura F. G. Salem

и другие.

Antioxidants, Год журнала: 2025, Номер 14(3), С. 329 - 329

Опубликована: Март 11, 2025

Plants face various stresses, particularly water deficit, which negatively impacts photosynthesis, growth, and development, thereby limiting agricultural production. Utilizing growth regulators, such as amino acids polyamines, to enhance osmotic stress tolerance is a crucial area of research in sustainable agriculture. This study investigates the impact arginine spermine treatments on attributes, enzymatic non-enzymatic antioxidants, photosynthetic pigments, protein lipid peroxidation, yield traits fenugreek plants under both normal drought conditions. The results indicate that conditions significantly reduce morphological characteristics, leaf traits. However, application enhances these parameters, with showing more pronounced effect. Additionally, boost antioxidant enzymes activities improve levels antioxidants osmolytes, contributing better performance. Principal component analysis confirms alters plant physiology, increasing proline malondialdehyde levels, while alleviate by enhancing activity osmolyte accumulation. current investigation aims evaluate effectiveness attributes conditions, focusing their comparative efficacy.

Язык: Английский

Процитировано

1
Spermine driven water deficit tolerance in early growth phases of sweet corn genotypes under hydroponic cultivation DOI Creative Commons
Tahoora Batool Zargar, Mawia Sobh, Oqba Basal

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 13, 2025

Abstract Sweet corn is highly susceptible to water deprivation, making it crucial identify effective strategies for enhancing its tolerance deficit conditions. This study investigates the novel application of Spermine as a bio-stimulant improve sweet ( Zea mays L. var. saccharata ) resilience under hydroponic Four genotypes (Dessert, Messenger, Tyson, and Royalty) were treated with (0.2 mM foliar application), polyethylene glycol 6000 (8% 12%), their combinations. The impacts on growth parameters, photosynthetic performance, oxidative stress markers evaluated. significantly enhanced biomass counteracting severe reductions caused by PEG-induced deprivation. In Dessert Tyson genotypes, total increased 145%, while 118% in Messenger 110% Royalty when However, treatment did not recorded higher differences compared control non genotype, root length 36.6% combined 12% PEG alone. also mitigated shoot length, improved 90.6% specific leaf area, notable 272.6% increase deficit. Photosynthetic including chlorophyll carotenoid levels, was enhanced, 103.1% relative content reduced damage, indicated 48.7% decrease malondialdehyde levels peroxidase activity, antioxidant defense quantum efficiency Photosystem II, which deficit, showed substantial improvement treatment, increases 107.2% 99.4% moderate These results highlight potential an strategy water-limited conditions, offering approach sustainable crop management.

Язык: Английский

Процитировано

0
Physiological and Flavonoid Metabolic Responses of Black Locust Leaves to Drought Stress in the Loess Plateau of China DOI Open Access
Yan Wang, Peng Ning, Binbin Liu

и другие.

Forests, Год журнала: 2025, Номер 16(4), С. 695 - 695

Опубликована: Апрель 17, 2025

Drought threatens the stability of artificial black locust forests on Loess Plateau, yet there is limited research physiological and metabolic responses mature to drought stress. This study employed a throughfall exclusion system—i.e., moderate (40% reduction), extreme (80% 0% reduction for control—to analyze leaf microstructure, relative water content (RWC), osmotic adjustment substances, hormone levels, flavonoid metabolites in under controlled The results demonstrated that as stress intensified, stomatal aperture density decreased, while trichome length exhibited significant increases. MDA, proline, IAA, substances (soluble protein, reducing sugar, total sugar) first increased then decreased intensified. A 245 compounds were identified through metabolomic analysis, among which 91 differential expression treatments. Notably, 37 flavonoids, including flavonols glycosylated derivatives, consistently upregulated. These findings suggest can lead accumulation flavonoids. explored trees stress, offering insights selecting drought-resistant species vegetation restoration informing ecological management practices arid regions.

Язык: Английский

Процитировано

0
Harnessing Belowground Interaction: Re-analyzing the Role of Rhizosphere Microbiome in Plant–Pathogen Interaction Under Water Stress DOI

Sumanti Gupta,

Shelly Sinha,

Anirban Bhar

и другие.

Journal of Plant Growth Regulation, Год журнала: 2024, Номер unknown

Опубликована: Сен. 24, 2024

Язык: Английский

Процитировано

0
Cloning, In Silico Characterization, and Physiochemical Analysis of the Zea mays PR-1 Gene: Implications for Developing Fungi-Resistant Transgenic Plants DOI
Muhammad Saleem,

Zaher Uddin Babar,

Sumaia Saif

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 27, 2024

Abstract Pathogenesis-related protein 1 (PR-1) is a critical component of plant defense mechanisms, exhibiting significant antifungal activity. This study focused on the PR-1 from Zea mays and five other species wheat, barley, rice, oats, rye to explore their physicochemical, structural, functional characteristics. The gene was successfully isolated Z. amplified using Polymerase Chain Reaction (PCR). Following cloning into pET15b vector, expressed in BL21-DE3 (E. coli) cells. Induction with 1mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) for 4 hours at 37°C led successful expression, as confirmed by 12% SDS-PAGE. exhibited resistance against broad spectrum oomycete pathogens, demonstrating its potential developing fungi-resistant transgenic plants. revealed that proteins different shared high sequence similarity, identities ranging 65–90%. However, physicochemical properties varied based origin. cereals (maize, rice) were found be more hydrophobic compared those grasses (oats rye). Structurally, all adopted similar three-dimensional conformation, featuring central beta-barrel surrounded alpha-helices. Functional assays six activity diverse array fungal pathogens. mays, along species, shows agricultural biotechnology. By incorporating these genes crop plants, it possible enhance thereby advancing development resilient sustainable systems.

Язык: Английский

Процитировано

0