Natural Source of Drugs Targeting Central Nervous System Tumors—Focus on NAD(P)H Oxidoreductase 1 (NQO1) Activity
Brain Sciences,
Год журнала:
2025,
Номер
15(2), С. 132 - 132
Опубликована: Янв. 29, 2025
Central
nervous
system
(CNS)
tumors
involve
a
large
and
diverse
group
of
malignancies
that
arise
from
various
cell
types
within
the
brain
tissue.
Although
there
are
advances
in
treatments,
CNS
still
remain
challenging,
due
to
their
complex
biology
delicate
nature
surrounding
NAD(P)H
O=oxidoreductase
1
(NQO1)
is
an
enzyme
plays
critical
role
detoxification
quinones,
protecting
cells
oxidative
stress.
In
this
often
overexpressed,
which
contributes
resistance
tumor
chemotherapy
by
enhancing
antioxidant
defenses.
NQO1
influences
progression
affecting
downstream
signaling
pathways,
such
as
those
involving
transcription
factor
SNAIL,
well
others
associated
with
behavior.
Plants
represent
valuable
source
numerous
constituents
different
chemical
structures
known
affect
molecular
pathways
pathologies.
Язык: Английский
The Occurrence, Uses, Biosynthetic Pathway, and Biotechnological Production of Plumbagin, a Potent Antitumor Naphthoquinone
Molecules,
Год журнала:
2025,
Номер
30(7), С. 1618 - 1618
Опубликована: Апрель 4, 2025
Plumbagin
is
an
important
naphthoquinone
with
potent
anticancer
properties
besides
multitudinous
uses
in
healthcare.
It
produced
a
limited
number
of
species
and
families
but
mostly
the
roots
Plumbaginaceae
family
members.
The
biosynthetic
pathway
genes
that
regulate
plumbagin
synthesis
are
not
completely
known,
details
these
being
revealed.
Several
species,
including
Plumbago,
Drosera,
others,
uprooted
for
extraction
by
pharmaceutical
industries,
leading
to
destruction
natural
habitats.
industry
therefore
facing
acute
shortage
plant
material.
This
necessitates
enhancing
accumulation
using
suspensions
hairy
meet
market
demands.
Many
factors,
such
as
aggregate
size
inoculum,
stability
culture,
sequential
effects
elicitors,
immobilization,
permeabilization,
have
been
demonstrated
act
synergistically
markedly
augment
accumulation.
Hairy
root
cultures
can
be
used
large-scale
production,
growth,
accumulation,
exploration
their
efficacy
now
imperative.
secretion
compounds
into
spent
medium
situ
adsorption
via
resin
has
remarkable
potential,
this
thoroughly
exploited.
Improvements
quality
biomass,
selection
cell
lines,
production
bioreactors
thus
far
sporadic,
parameters
need
further
In
review,
we
report
advances
made
relating
importance
stable
line
long-term
cultures,
plumbagin,
its
semicontinuous
total
recycling
different
types
bioreactors.
Such
might
pave
way
industrial
exploitation.
steps
currently
understood
also
aid
us
isolating
relevant
order
examine
overexpression
or
heterologous
downregulation
edit
genome
CRISPR-Cas9
technology
enhance
plumbagin.
Its
potential
molecule
mode
action
amply
demonstrated,
exploited
clinics
due
insolubility
water
highly
lipophilic
nature.
Plumbagin-loaded
nanoemulsions,
plumbagin–silver,
albumin
nanoparticle
formulations
overcome
problems
solubility
tried
improve
bioavailability
antiproliferative
activities,
discussed
current
paper.
Язык: Английский
Synergistic Inhibition of Colon Cancer Cell Proliferation via p53, Bax, and Bcl-2 Modulation by Curcumin and Plumbagin Combination
ACS Omega,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 29, 2025
Cancer
is
a
major
contributor
to
global
morbidity
and
mortality.
Among
the
different
forms
of
cancer,
colorectal
cancer
(CRC)
third
most
frequently
diagnosed
in
men
second
common
type
women
globally.
We
aimed
explore
possible
synergistic
anticancer
potential
curcumin
(Cur)
plumbagin
(PL)
human
colon
cell
line
(HCT-116).
The
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide
(MTT)/cytotoxicity
assay
revealed
IC50
values
7.7
7.5
μM
for
Cur
PL,
respectively,
as
separate
entity.
However,
combined
treatment
+
PL
significantly
enhanced
growth
inhibitory
compared
with
solitary
treatments
an
value
6.8
μM.
also
led
induction
apoptosis
by
41%,
cycle
arrest
at
G2/M
phase,
while
Bax
p53
genes
were
found
be
upregulated
Bcl-2
gene
was
downregulated
untreated/solvent
control.
Furthermore,
elevated
reactive
oxygen
species
(ROS)
production
59%
resulted
decline
mitochondrial
membrane
(MMP)
Catalase
superoxide
dismutase
(SOD)
activities
reduced,
leading
lipid
peroxidation
(LPO)
compromised
integrity,
which
confirmed
4',6-diamidino-2-phenylindole
(DAPI)
propoidium
iodide
(PI)
staining
noted.
Our
vitro
data
further
supported
molecular
docking,
showed
higher
binding
energy
proteins
(Bax,
Bcl-2,
p53)
PL.
Overall,
our
findings
highlight
potent
effects
combination,
can
exploited
combination
therapy
CRC.
Язык: Английский