Synergistic Inhibition of Colon Cancer Cell Proliferation via p53, Bax, and Bcl-2 Modulation by Curcumin and Plumbagin Combination DOI Creative Commons
Iftikhar Ahmad, Sameer Ahmad, Md Abdus Samad

и другие.

ACS Omega, Год журнала: 2025, Номер unknown

Опубликована: Апрель 29, 2025

Cancer is a major contributor to global morbidity and mortality. Among the different forms of cancer, colorectal cancer (CRC) third most frequently diagnosed in men second common type women globally. We aimed explore possible synergistic anticancer potential curcumin (Cur) plumbagin (PL) human colon cell line (HCT-116). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)/cytotoxicity assay revealed IC50 values 7.7 7.5 μM for Cur PL, respectively, as separate entity. However, combined treatment + PL significantly enhanced growth inhibitory compared with solitary treatments an value 6.8 μM. also led induction apoptosis by 41%, cycle arrest at G2/M phase, while Bax p53 genes were found be upregulated Bcl-2 gene was downregulated untreated/solvent control. Furthermore, elevated reactive oxygen species (ROS) production 59% resulted decline mitochondrial membrane (MMP) Catalase superoxide dismutase (SOD) activities reduced, leading lipid peroxidation (LPO) compromised integrity, which confirmed 4',6-diamidino-2-phenylindole (DAPI) propoidium iodide (PI) staining noted. Our vitro data further supported molecular docking, showed higher binding energy proteins (Bax, Bcl-2, p53) PL. Overall, our findings highlight potent effects combination, can exploited combination therapy CRC.

Язык: Английский

Natural Source of Drugs Targeting Central Nervous System Tumors—Focus on NAD(P)H Oxidoreductase 1 (NQO1) Activity DOI Creative Commons
Nikola M. Stojanović,

M Mitić,

Jovan Ilić

и другие.

Brain Sciences, Год журнала: 2025, Номер 15(2), С. 132 - 132

Опубликована: Янв. 29, 2025

Central nervous system (CNS) tumors involve a large and diverse group of malignancies that arise from various cell types within the brain tissue. Although there are advances in treatments, CNS still remain challenging, due to their complex biology delicate nature surrounding NAD(P)H O=oxidoreductase 1 (NQO1) is an enzyme plays critical role detoxification quinones, protecting cells oxidative stress. In this often overexpressed, which contributes resistance tumor chemotherapy by enhancing antioxidant defenses. NQO1 influences progression affecting downstream signaling pathways, such as those involving transcription factor SNAIL, well others associated with behavior. Plants represent valuable source numerous constituents different chemical structures known affect molecular pathways pathologies.

Язык: Английский

Процитировано

0

The Occurrence, Uses, Biosynthetic Pathway, and Biotechnological Production of Plumbagin, a Potent Antitumor Naphthoquinone DOI Creative Commons
P. B. Kavi Kishor, Bangaru Naidu Thaddi, G. Rajasheker

и другие.

Molecules, Год журнала: 2025, Номер 30(7), С. 1618 - 1618

Опубликована: Апрель 4, 2025

Plumbagin is an important naphthoquinone with potent anticancer properties besides multitudinous uses in healthcare. It produced a limited number of species and families but mostly the roots Plumbaginaceae family members. The biosynthetic pathway genes that regulate plumbagin synthesis are not completely known, details these being revealed. Several species, including Plumbago, Drosera, others, uprooted for extraction by pharmaceutical industries, leading to destruction natural habitats. industry therefore facing acute shortage plant material. This necessitates enhancing accumulation using suspensions hairy meet market demands. Many factors, such as aggregate size inoculum, stability culture, sequential effects elicitors, immobilization, permeabilization, have been demonstrated act synergistically markedly augment accumulation. Hairy root cultures can be used large-scale production, growth, accumulation, exploration their efficacy now imperative. secretion compounds into spent medium situ adsorption via resin has remarkable potential, this thoroughly exploited. Improvements quality biomass, selection cell lines, production bioreactors thus far sporadic, parameters need further In review, we report advances made relating importance stable line long-term cultures, plumbagin, its semicontinuous total recycling different types bioreactors. Such might pave way industrial exploitation. steps currently understood also aid us isolating relevant order examine overexpression or heterologous downregulation edit genome CRISPR-Cas9 technology enhance plumbagin. Its potential molecule mode action amply demonstrated, exploited clinics due insolubility water highly lipophilic nature. Plumbagin-loaded nanoemulsions, plumbagin–silver, albumin nanoparticle formulations overcome problems solubility tried improve bioavailability antiproliferative activities, discussed current paper.

Язык: Английский

Процитировано

0

Synergistic Inhibition of Colon Cancer Cell Proliferation via p53, Bax, and Bcl-2 Modulation by Curcumin and Plumbagin Combination DOI Creative Commons
Iftikhar Ahmad, Sameer Ahmad, Md Abdus Samad

и другие.

ACS Omega, Год журнала: 2025, Номер unknown

Опубликована: Апрель 29, 2025

Cancer is a major contributor to global morbidity and mortality. Among the different forms of cancer, colorectal cancer (CRC) third most frequently diagnosed in men second common type women globally. We aimed explore possible synergistic anticancer potential curcumin (Cur) plumbagin (PL) human colon cell line (HCT-116). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)/cytotoxicity assay revealed IC50 values 7.7 7.5 μM for Cur PL, respectively, as separate entity. However, combined treatment + PL significantly enhanced growth inhibitory compared with solitary treatments an value 6.8 μM. also led induction apoptosis by 41%, cycle arrest at G2/M phase, while Bax p53 genes were found be upregulated Bcl-2 gene was downregulated untreated/solvent control. Furthermore, elevated reactive oxygen species (ROS) production 59% resulted decline mitochondrial membrane (MMP) Catalase superoxide dismutase (SOD) activities reduced, leading lipid peroxidation (LPO) compromised integrity, which confirmed 4',6-diamidino-2-phenylindole (DAPI) propoidium iodide (PI) staining noted. Our vitro data further supported molecular docking, showed higher binding energy proteins (Bax, Bcl-2, p53) PL. Overall, our findings highlight potent effects combination, can exploited combination therapy CRC.

Язык: Английский

Процитировано

0