Silver Nanoparticles Synthesized From Centella asiatica Extract and Asiatic Acid for Enhanced Biofilm Eradication of Streptococcus Associated With Oral Diseases DOI Creative Commons

Sukanlaya Leejae,

Wattana Pelyuntha, G. Roopa Lavanya

и другие.

Scientifica, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

The biofilm‐forming ability of Streptococcus species, particularly mutans , is a key factor in the pathogenesis dental caries and periodontitis. Current treatments often exhibit limitations such as incomplete biofilm eradication cytotoxicity to host tissues, highlighting need for innovative biocompatible therapeutic approaches. Therefore, this study aimed investigate potential Centella asiatica ethanolic extract, its bioactive triterpenoids (asiatic acid madecassic acid), silver nanoparticles (AgNPs) synthesized from extract an alternative strategy targeting S. biofilms. antibacterial antibiofilm activities AgNPs against species were evaluated, alongside assessments on human gingival fibroblast (HGF‐1) cells using MTT assay. exhibited superior antimicrobial efficacy compared with significantly lower minimum inhibitory concentration (MIC) bactericidal (MBC) values (62.5/125 µg/mL) ATCC 25175 mitis 49456, their potent activity. Moreover, achieved rapid disruption, reducing biomass by 76% within 12 h at 1/2 × MIC, outperforming triterpenoids. Scanning electron microscopy further revealed substantial extracellular polymeric substance degradation structural disruption upon AgNP treatment, confirming pronounced efficacy. In addition, demonstrated favorable biocompatibility, maintaining 68% cell viability cells, suggesting optimal balance between potency cytotoxicity. synergistic interaction C. phytochemicals enhanced nonfunctionalized AgNPs. These findings underscore –based novel, plant‐derived nanotechnological approach managing oral infections caused species. This not only contributes development strategies but also paves way future clinical applications healthcare.

Язык: Английский

Silver Nanoparticles Synthesized From Centella asiatica Extract and Asiatic Acid for Enhanced Biofilm Eradication of Streptococcus Associated With Oral Diseases DOI Creative Commons

Sukanlaya Leejae,

Wattana Pelyuntha, G. Roopa Lavanya

и другие.

Scientifica, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

The biofilm‐forming ability of Streptococcus species, particularly mutans , is a key factor in the pathogenesis dental caries and periodontitis. Current treatments often exhibit limitations such as incomplete biofilm eradication cytotoxicity to host tissues, highlighting need for innovative biocompatible therapeutic approaches. Therefore, this study aimed investigate potential Centella asiatica ethanolic extract, its bioactive triterpenoids (asiatic acid madecassic acid), silver nanoparticles (AgNPs) synthesized from extract an alternative strategy targeting S. biofilms. antibacterial antibiofilm activities AgNPs against species were evaluated, alongside assessments on human gingival fibroblast (HGF‐1) cells using MTT assay. exhibited superior antimicrobial efficacy compared with significantly lower minimum inhibitory concentration (MIC) bactericidal (MBC) values (62.5/125 µg/mL) ATCC 25175 mitis 49456, their potent activity. Moreover, achieved rapid disruption, reducing biomass by 76% within 12 h at 1/2 × MIC, outperforming triterpenoids. Scanning electron microscopy further revealed substantial extracellular polymeric substance degradation structural disruption upon AgNP treatment, confirming pronounced efficacy. In addition, demonstrated favorable biocompatibility, maintaining 68% cell viability cells, suggesting optimal balance between potency cytotoxicity. synergistic interaction C. phytochemicals enhanced nonfunctionalized AgNPs. These findings underscore –based novel, plant‐derived nanotechnological approach managing oral infections caused species. This not only contributes development strategies but also paves way future clinical applications healthcare.

Язык: Английский

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