Screening, identification, and application of aptamers against allergens in food matrices DOI
Chao Zhu, Hongxia Du, Hao Liu

и другие.

Critical Reviews in Food Science and Nutrition, Год журнала: 2024, Номер unknown, С. 1 - 26

Опубликована: Дек. 11, 2024

Food allergies have become one of the most pressing issues in food safety and public health globally along with their incidence increasing recent years. The reliable recognition allergens from different sources, especially food-hidden allergens, is essential for preventing controlling allergies. Recently, aptamers, as emerging elements, gained considerable attention allergy, detection allergens. This review systematically summarizes latest progress screening, identification, application aptamers against over past five We first introduce a brief overview allergy followed by detailed focus on aptamers' research broadly based major categories Big-8 allergens: highlighting newly screened applied systematic evolution ligands exponential enrichment (SELEX) strategies, emphasizing practical applications including aptasensors, inhibitors, or affinity adsorptions. Finally, remaining challenges future exploitations faced are comprehensively discussed depicted. holds promise inspiring broader range researchers to gain an in-depth understanding assisted aptamer facilitate improved biochemical analyses successful application.

Язык: Английский

Miniaturized solid-phase extraction techniques in sample preparation applied to food matrices: A review DOI Creative Commons
Ana Jano, Adrián Fuente-Ballesteros, Ana M. Ares

и другие.

Microchemical Journal, Год журнала: 2025, Номер 213, С. 113794 - 113794

Опубликована: Апрель 27, 2025

Язык: Английский

Процитировано

1

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods DOI Creative Commons
Norberto A. Guzman,

Andrea Guzman

International Journal of Translational Medicine, Год журнала: 2024, Номер 4(2), С. 309 - 333

Опубликована: Май 31, 2024

Many ailments can be diagnosed while they are asymptomatic, meaning that the patient has no signs or symptoms of a progressing disease. If caught in their initial stage formation, these disorders effectively treated, leading to successful outcomes; curative therapies halt illnesses from advancing, thus improving quality life and long-term survival patient. Still, cutting-edge upgrades precision technologies necessary for early, reliable, affordable, rapid disease detection, but also vital well-being people future global public health. The emerging role utility non-invasive repeatable diagnostic test approaches detection health conditions have been exemplified by liquid biopsies based on genomic biomarkers. As such, biological fluids permit any measurable molecular indicator signature (e.g., proteins) provide valuable information an individual’s wellness and/or Among bodily secretions used diagnostics is sputum, complex viscous gel-like biopolymeric network gained growing recognition as rich source biomarkers airway infections pulmonary diseases, serves determinant reveal other illnesses. per World Health Organization, burden respiratory exacerbated factors ranging considerable subjection air pollution occupational contaminants tobacco smoking second-hand smoke, addition poor socio-economic status. Due likely increase determinants, tract rise, affecting many individuals, putting stress healthcare facilities services worldwide. aim this study was perform narrative review sputum constituents with emphasis proteins glycoproteins assessed possible lung organ diseases. A search conducted using mucus, proteomics, biomarkers, point-of-care testing keywords employing Google, PubMed (MEDLINE), Web Science, selecting most referenced related papers last decade. We, therefore, highlight need use expectorated induced specimens routine sample protein diagnose chronic disorders, predict inflammation progression, well monitor effectiveness treatments. Further, we discuss urgent fast reliable methods detect quantify crucial specimens, limitations faced when dealing matrices.

Язык: Английский

Процитировано

3

Human Sputum Proteomics: Advancing Non-Invasive Diagnosis of Respiratory Diseases with Enhanced Biomarker Analysis Methods DOI Open Access
Norberto A. Guzman,

Andrea Guzman

Опубликована: Апрель 29, 2024

Many ailments can be diagnosed while they are asymptomatic, meaning that the patient has no signs or symptoms of a progressing disease. If caught in their initial stage formation, these maladies effectively treated, leading to successful outcomes; curative therapies halt illnesses from advancing, thus improving quality life, and long-term survival patient. Still cutting-edge upgrades precision technologies necessary for early, reliable, affordable, rapid disease detection, but also vital well-being people future global public health. The emerging role utility non-invasive repeatable diagnostic test approaches detection health conditions been liquid biopsies based on genomic biomarkers. As such, biological fluids permit any measurable molecular indicator signature (e.g., proteins), provide valuable information individual’s wellness and/or Among bodily secretions used diagnostics is sputum, complex viscous gel-like biopolymeric network, gained growing recognition as rich source biomarkers airway infections, pulmonary diseases, serves determinant reveal other illnesses. per World Health Organization, burden respiratory exacerbated by factors, ranging considerable subjection air pollution occupational contaminants, tobacco smoking second-hand smoke, addition poor socio-economic status. Due likely increase determinants, tract rise, affecting many individuals, putting stress healthcare facilities services worldwide. We therefore highlight need use expectorated induced sputum specimens routine sample testing protein diagnose chronic maladies, predict inflammation progression, well monitor effectiveness treatments. Further, we discuss urgency fast reliable point-of-care methods detect quantify crucial specimens, limitations faced when dealing with matrices.

Язык: Английский

Процитировано

2

A Comprehensive Review on Capillary Electrophoresis–Mass Spectrometry in Advancing Biomolecular Research DOI Creative Commons
Laura Pont, María Vergara‐Barberán, Enrique Javier Carrasco‐Correa

и другие.

Electrophoresis, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 7, 2024

ABSTRACT This review provides an in‐depth exploration of capillary electrophoresis–mass spectrometry (CE–MS) in biomolecular research from 2020 to 2024. CE–MS emerges as a versatile and powerful tool due its numerous advantages, facilitating the analysis various biomolecules, including proteins, peptides, oligonucleotides, other metabolites, such lipids, carbohydrates, or amines, among others. The extends CE modes interfaces for coupling, offering comprehensive insights into their applications within research. Furthermore, it effectively summarizes conditions employed while also addressing critical aspects sample preparation requirements. Despite highlights gap between discovery practical implementation, underscoring need large‐scale validation method standardization fully realize potential

Язык: Английский

Процитировано

1

The potential of new solvents and materials in the context of green analytical chemistry DOI Creative Commons
Lourdes Ramos,

Jose Manuel Herreros-Martínez,

Belén Gómara

и другие.

Advances in Sample Preparation, Год журнала: 2023, Номер 8, С. 100096 - 100096

Опубликована: Сен. 25, 2023

Язык: Английский

Процитировано

1

On-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry for the determination of SARS-CoV-2 nucleocapsid protein DOI Creative Commons
Hiba Salim, Laura Pont, Estela Giménez

и другие.

Microchemical Journal, Год журнала: 2024, Номер unknown, С. 112505 - 112505

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

0

Screening, identification, and application of aptamers against allergens in food matrices DOI
Chao Zhu, Hongxia Du, Hao Liu

и другие.

Critical Reviews in Food Science and Nutrition, Год журнала: 2024, Номер unknown, С. 1 - 26

Опубликована: Дек. 11, 2024

Food allergies have become one of the most pressing issues in food safety and public health globally along with their incidence increasing recent years. The reliable recognition allergens from different sources, especially food-hidden allergens, is essential for preventing controlling allergies. Recently, aptamers, as emerging elements, gained considerable attention allergy, detection allergens. This review systematically summarizes latest progress screening, identification, application aptamers against over past five We first introduce a brief overview allergy followed by detailed focus on aptamers' research broadly based major categories Big-8 allergens: highlighting newly screened applied systematic evolution ligands exponential enrichment (SELEX) strategies, emphasizing practical applications including aptasensors, inhibitors, or affinity adsorptions. Finally, remaining challenges future exploitations faced are comprehensively discussed depicted. holds promise inspiring broader range researchers to gain an in-depth understanding assisted aptamer facilitate improved biochemical analyses successful application.

Язык: Английский

Процитировано

0