Mitochondrial Ribosomal Proteins and Cancer
Medicina,
Год журнала:
2025,
Номер
61(1), С. 96 - 96
Опубликована: Янв. 9, 2025
Mitochondria
play
key
roles
in
maintaining
cell
life
and
function,
their
dysfunction
can
lead
to
damage.
Mitochondrial
ribosomal
proteins
(MRPs)
are
encoded
by
nuclear
genes
assembled
within
the
mitochondria.
MRPs
pivotal
components
of
mitochondrial
ribosomes,
which
responsible
for
translating
13
DNA-encoded
essential
respiratory
chain.
Recent
studies
have
underscored
importance
cancer
biology,
revealing
altered
expression
patterns
various
types
potential
as
both
prognostic
biomarkers
therapeutic
targets.
Herein,
we
review
current
knowledge
regarding
multiple
functions
structure
ribosome
apoptosis,
implications
susceptibility
progression,
innovative
strategies
being
developed
target
mitoribosome
biogenesis
therapy.
This
comprehensive
overview
aims
provide
insights
into
role
biology
highlight
promising
future
precision
oncology.
Язык: Английский
A correlative workflow streamlines synaptic imaging by cryo-electron tomography
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Март 3, 2025
Abstract
Despite
decades
of
intense
research,
the
molecular
organization
synapse
is
not
well
understood.
To
address
this
issue,
we
sought
to
develop
a
method
for
systematic
imaging
synapses
by
cryo-electron
tomography
(cryo-ET),
technology
capable
mapping
cellular
architecture
at
resolution.
Thinning
samples
cryo-focused
ion
beam
milling
prerequisite
high-quality
cryo-ET
imaging,
but
process
needs
be
guided
structures
interest.
allow
robust
synaptic
targeting,
established
correlative
cryo-light/electron
microscopy
approach
which
are
fluorescently
labeled
in
minimally
invasive
manner,
using
synthetic
binder
postsynaptic
scaffold
PSD-95
and
antibodies
against
presynaptic
protein
Synaptotagmin-1.
Cryo-ET
sites
colocalization
consistently
revealed
excitatory
synapses.
Our
allows
structural
studies
complexes
situ
,
facilitating
investigations
Язык: Английский
New dimensions in acidocalcisome research: the potential of cryo-EM to uncover novel aspects of protozoan parasite physiology
mBio,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 8, 2025
ABSTRACT
Cryo-electron
microscopy
(cryo-EM)
has
revolutionized
structural
biology
by
enabling
high-resolution,
near-native
visualization
of
macromolecular
structures
and
entire
cells.
Its
application
to
etiologic
agents
diseases
is
an
expanding
field,
particularly
for
those
caused
viruses
or
unicellular
eukaryotes,
such
as
protozoan
parasites
fungi.
This
review
focuses
on
acidocalcisomes—ion-rich,
multifunctional
organelles
essential
cell
physiology
survival
in
several
pathogens.
The
structure
function
these
are
examined
through
a
range
electron
techniques,
using
Trypanosoma
cruzi
model.
advantages
limitations
the
methods
employed
study
acidocalcisome
morphofunctional
organization—such
chemical
fixation,
plunge
high-pressure
freezing,
cryo-electron
vitrified
sections
(CEMOVIS),
freeze-drying,
freeze
substitution,
tomography,
microanalysis
X
rays
inelastic
scattered
electrons—are
discussed,
alongside
their
contributions
our
current
understanding
function.
Recent
advances
cryo-EM
its
potential
address
longstanding
questions
fill
existing
gaps
parasite
ion
mobilization
mechanisms
also
discussed.
Язык: Английский