Dual immunoplatform to assess senescence biomarkers TIMP-1 and GDF-15: Advancing in the understanding of colorectal cancer DOI Creative Commons
Sandra Tejerina-Miranda, María Gamella, Marı́a Pedrero

и другие.

Electrochimica Acta, Год журнала: 2024, Номер 503, С. 144822 - 144822

Опубликована: Авг. 3, 2024

In this work, we report the development of electrochemical bioplatforms for both single determination TIMP-1 and simultaneous GDF-15, two biomarkers cellular senescence prognosis in colorectal cancer (CRC). The designed immunoplatforms rely on a sandwich-type configuration labeled with horseradish peroxidase (HRP) built magnetic microsupports involve pair specific antibodies to capture detect each target protein. Amperometric readout was performed upon trapping bioconjugates surface either or dual disposable carbon electrodes, using hydroquinone/hydrogen peroxide (HQ/H2O2) system. attractive analytical performance bioplatform, achieving LOD 13.0 pg mL‒1 dynamic range between 43.4 2500 mL‒1, led us exploit it cell extracts tissues CRC samples. results showed possibility discriminating metastatic capabilities cells detecting patients, after just simple dilution only 60 min. multiplexing feasibility developed immunoplatform allowed implementation assay (LOD 19.2 mL‒1) GDF-15 16.6 mL‒1). platform used analysis samples (plasma secretomes) 75 min very protocol. These characteristics make tool face particularly challenging samples, such as secretomes plasma from due required sensitivity, thus providing better snapshot CRC.

Язык: Английский

Cu2-xSe@Bi2MoO6 nanozyme-based immunoassay for the colorimetric detection of walnut allergen in foods DOI
Shasha Wang,

Lingjie Sheng,

Yang Lu

и другие.

Food Chemistry, Год журнала: 2025, Номер 471, С. 142808 - 142808

Опубликована: Янв. 7, 2025

Язык: Английский

Процитировано

1
Electrochemical tracking of gluten in marketed foods by using a recombinant antibody fragment based-platform DOI
Víctor Ruiz‐Valdepeñas Montiel, Eduardo Garcia‐Calvo, María Gamella

и другие.

Talanta, Год журнала: 2025, Номер 288, С. 127747 - 127747

Опубликована: Фев. 18, 2025

Язык: Английский

Процитировано

0
Dual immunoplatform to assess senescence biomarkers TIMP-1 and GDF-15: Advancing in the understanding of colorectal cancer DOI Creative Commons
Sandra Tejerina-Miranda, María Gamella, Marı́a Pedrero

и другие.

Electrochimica Acta, Год журнала: 2024, Номер 503, С. 144822 - 144822

Опубликована: Авг. 3, 2024

In this work, we report the development of electrochemical bioplatforms for both single determination TIMP-1 and simultaneous GDF-15, two biomarkers cellular senescence prognosis in colorectal cancer (CRC). The designed immunoplatforms rely on a sandwich-type configuration labeled with horseradish peroxidase (HRP) built magnetic microsupports involve pair specific antibodies to capture detect each target protein. Amperometric readout was performed upon trapping bioconjugates surface either or dual disposable carbon electrodes, using hydroquinone/hydrogen peroxide (HQ/H2O2) system. attractive analytical performance bioplatform, achieving LOD 13.0 pg mL‒1 dynamic range between 43.4 2500 mL‒1, led us exploit it cell extracts tissues CRC samples. results showed possibility discriminating metastatic capabilities cells detecting patients, after just simple dilution only 60 min. multiplexing feasibility developed immunoplatform allowed implementation assay (LOD 19.2 mL‒1) GDF-15 16.6 mL‒1). platform used analysis samples (plasma secretomes) 75 min very protocol. These characteristics make tool face particularly challenging samples, such as secretomes plasma from due required sensitivity, thus providing better snapshot CRC.

Язык: Английский

Процитировано

1