Juvenile
hormone
(JH)
is
important
to
maintain
insect
larval
status;
however,
its
cell
membrane
receptor
has
not
been
identified.
Using
the
lepidopteran
Helicoverpa
armigera
(cotton
bollworm),
a
serious
agricultural
pest,
as
model,
we
determined
that
tyrosine
kinases
(RTKs)
cadherin
96ca
(CAD96CA)
and
fibroblast
growth
factor
homologue
(FGFR1)
function
JH
receptors
by
their
roles
in
JH-regulated
gene
expression,
status
maintaining,
calcium
increase,
phosphorylation
of
intracellular
MET1
cofactor
Taiman,
high
affinity
III.
Gene
knockout
Cad96ca
Fgfr1
CRISPR/Cas9
embryo
knockdown
various
cells,
overexpression
CAD96CA
FGFR1
mammalian
HEK-293T
cells
all
supported
transmitting
signal
receptors.
Proceedings of the National Academy of Sciences,
Год журнала:
2021,
Номер
118(39)
Опубликована: Сен. 20, 2021
It
is
well
documented
that
the
juvenile
hormone
(JH)
can
function
as
a
gonadotropic
stimulates
vitellogenesis
by
activating
production
and
uptake
of
vitellogenin
in
insects.
Here,
we
describe
phenotype
associated
with
mutations
Frontiers in Physiology,
Год журнала:
2022,
Номер
12
Опубликована: Фев. 10, 2022
In
the
field
of
insect
endocrinology,
juvenile
hormone
(JH)
is
one
most
wondrous
entomological
terms.
As
a
unique
sesquiterpenoid
produced
and
released
by
endocrine
gland,
corpus
allatum
(CA),
JH
critical
regulator
in
multiple
developmental
physiological
processes,
such
as
metamorphosis,
reproduction,
behavior.
Benefited
from
precise
genetic
interventions
simplicity,
fruit
fly,
PLoS Genetics,
Год журнала:
2022,
Номер
18(6), С. e1010292 - e1010292
Опубликована: Июнь 27, 2022
Juvenile
hormone
(JH)
acts
as
a
gonadotrophic
stimulating
insect
vitellogenesis
and
oogenesis.
Paracellular
transport
of
yolk
proteins
through
intercellular
channels
(patency)
in
the
follicular
epithelium
is
developmentally
regulated
evolutionarily
conserved
process
during
vitellogenesis.
However,
mechanisms
underlying
patency
opening
are
poorly
understood.
Using
migratory
locust
Locusta
migratoria
model
system,
we
report
here
that
JH-regulated
remodeling
zonula
adherens
(ZA),
belt-like
junction
maintaining
physical
linking
between
follicle
cells
controlled
patency.
JH
triggered
phosphorylation
Partitioning
defective
protein
3
(Par3)
via
signaling
cascade
including
G
protein-coupled
receptor
(GPCR),
small
GTPase
Cell
division
cycle
42
(Cdc42)
atypical
Protein
kinase
C
(aPKC).
Par3
resulted
its
disassociation
from
β-Catenin,
cytoplasmic
partner
ZA
core
component
E-Cadherin.
Release
β-Catenin/E-Cadherin
complex
caused
disassembly
at
tricellular
contacts,
consequently
leading
to
enlargement.
This
study
provides
new
insight
into
how
stimulates
egg
production
inducing
paracellular
route
for
vitellogenin
crossing
barrier.
G3 Genes Genomes Genetics,
Год журнала:
2024,
Номер
14(7)
Опубликована: Апрель 29, 2024
Abstract
The
Drosophila
melanogaster
male
accessory
gland
(AG)
is
a
functional
analog
of
the
mammalian
prostate
and
seminal
vesicles
containing
two
secretory
epithelial
cell
types,
termed
main
secondary
cells.
This
tissue
responsible
for
making
secreting
fluid
proteins
other
molecules
that
contribute
to
successful
reproduction.
cells
this
are
binucleate
polyploid,
due
variant
cycles
include
endomitosis
endocycling
during
metamorphosis.
Here,
we
provide
evidence
additional
cycle
variants
in
tissue.
We
show
connected
by
ring
canals
form
after
penultimate
mitosis,
describe
an
post-eclosion
endocycle
required
maturation
dependent
on
juvenile
hormone
signaling.
present
D.
AG
undergo
unique
reprogramming
throughout
organ
development
results
step-wise
truncations
culminating
octoploid
nuclei
with
under-replicated
heterochromatin
mature
gland.
propose
as
model
study
developmental
hormonal
temporal
control
terminally
differentiating
tissues.
Ecotoxicology and Environmental Safety,
Год журнала:
2025,
Номер
289, С. 117656 - 117656
Опубликована: Янв. 1, 2025
The
increasing
utilization
of
antimony
(Sb)
in
manufacturing
industries
has
led
to
the
emergence
Sb
contamination
environment
as
a
significant
public
health
concern.
To
elucidate
toxicity
and
its
mechanism
action,
this
study
aimed
investigate
adverse
effects
on
cosmopolitan
insect,
housefly
(Musca
domestica),
under
whole
life
cycle
(from
embryonic
adult
stage)
exposure
through
examination
suite
parameters,
including
biological,
physiological,
behavioral,
molecular
endpoints.
A
range
concentrations,
moderate
(0.07
mM),
heavy
(0.7
extreme
(7
70
were
conducted
study.
results
indicated
that
houseflies
could
maintain
their
when
exposed
0.07
mM
Sb.
(0.7-70
mM)
resulted
effects,
developmental
retardation,
locomotor
inhibition,
gut
damage,
oxidative
stress,
mitochondrial
dysfunction
houseflies.
Significantly,
demonstrated
reproductive
houseflies,
evidenced
by
reduced
capacity,
DNA
ovarian
abnormalities.
disturbance
hormonal
synthesis
MAPK
pathway
induced
treatment
may
contribute
toxicity.
These
comprehensive
toxicological
data
provide
insight
into
prediction
assessment
ecological
risk
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(4), С. 1745 - 1745
Опубликована: Фев. 18, 2025
The
prolonged
use
of
pyrethroid
insecticides
for
controlling
the
plant
bug
Lygus
pratensis
has
led
to
upward
resistance.
This
study
aims
elucidate
molecular
mechanisms
and
potential
regulatory
pathways
associated
with
lambda-cyhalothrin
resistance
in
L.
pratensis.
In
this
study,
we
constructed
a
network
by
integrating
transcriptome
RNA-Seq
proteome
iTRAQ
sequencing
analyses
one
lambda-cyhalothrin-susceptible
strain
two
resistant
strains,
annotating
key
gene
families
detoxification,
identifying
differentially
expressed
genes
proteins,
screening
transcription
factors
involved
regulation
detoxification
metabolism,
examining
metabolic
A
total
82,919
unigenes
were
generated
following
assembly
data.
Of
these,
24,859
received
functional
annotations,
while
1064
differential
proteins
functionally
annotated,
1499
belonging
64
distinct
factor
identified.
Notably,
66
metabolism
classified
within
zf-C2H2,
Homeobox,
THAP,
MYB,
bHLH,
HTH,
HMG,
bZIP
families.
Co-analysis
revealed
that
CYP6A13
was
significantly
up-regulated
at
both
transcriptional
translational
levels.
GO
KEGG
enrichment
co-up-regulated
DEGs
DEPs
enriched
related
sphingolipid
Terpenoid
backbone
biosynthesis,
ABC
transporters,
RNA
transport,
peroxisome
function,
as
well
other
signaling
metabolism.
Conversely,
co-down-regulated
primarily
Oxidative
phosphorylation,
Fatty
acid
Neuroactive
ligand-receptor
interactions,
pertinent
growth
development.
results
series
physiological
biochemical
adaptations
exhibited
during
work
provided
theoretical
basis
further
analysis
mechanism
underlying
Juvenile
hormone
(JH)
is
important
to
maintain
insect
larval
status;
however,
its
cell
membrane
receptor
has
not
been
identified.
Using
the
lepidopteran
Helicoverpa
armigera
(cotton
bollworm),
a
serious
agricultural
pest,
as
model,
we
determined
that
tyrosine
kinases
(RTKs)
cadherin
96ca
(CAD96CA)
and
fibroblast
growth
factor
homologue
(FGFR1)
function
JH
receptors
by
their
roles
in
JH-regulated
gene
expression,
status
maintaining,
rapid
intracellular
calcium
increase,
phosphorylation
of
MET1
cofactor
Taiman,
high
affinity
III.
Gene
knockout
Cad96ca
Fgfr1
CRISPR/Cas9
embryo
knockdown
various
cells,
overexpression
CAD96CA
FGFR1
mammalian
HEK-293T
cells
all
supported
transmitting
signal
receptors.
Juvenile
hormone
(JH)
is
important
to
maintain
insect
larval
status;
however,
its
cell
membrane
receptor
has
not
been
identified.
Using
the
lepidopteran
Helicoverpa
armigera
(cotton
bollworm),
a
serious
agricultural
pest,
as
model,
we
determined
that
tyrosine
kinases
(RTKs)
cadherin
96ca
(CAD96CA)
and
fibroblast
growth
factor
homologue
(FGFR1)
function
JH
receptors
by
their
roles
in
JH-regulated
gene
expression,
status
maintaining,
rapid
intracellular
calcium
increase,
phosphorylation
of
MET1
cofactor
Taiman,
high
affinity
III.
Gene
knockout
Cad96ca
Fgfr1
CRISPR/Cas9
embryo
knockdown
various
cells,
overexpression
CAD96CA
FGFR1
mammalian
HEK-293T
cells
all
supported
transmitting
signal
receptors.
