Foods,
Год журнала:
2024,
Номер
13(19), С. 3036 - 3036
Опубликована: Сен. 25, 2024
Postharvest
ethylene
accumulation
and
lignification
are
significant
issues
affecting
the
storage
quality
of
fresh
faba
beans,
resulting
in
rapid
decline.
However,
there
is
still
a
lack
effective
preservation
methods
to
preserve
beans
during
storage.
This
study
aimed
investigate
regulation
using
high-efficiency
remover
(HEER),
examining
physiological
responses,
key
enzyme
activities,
transcriptomic
changes.
Results
showed
that
HEER
treatment
inhibited
lignification,
reducing
it
by
45%
lowering
respiratory
rate
pods
32.8%
Additionally,
suppressed
respiration
rates
activities
lignin
synthesis-related
enzymes,
including
phenylalanine
ammonia-lyase
(from
353.73
246.60
U/g),
cinnamic
acid-4-hydroxylase
635.86
125.00
4-coumarate:
coenzyme
A
ligase
1008.57
516.52
cinnamyl-alcohol
dehydrogenase
129.42
37.12
thus
slowing
accumulation.
During
storage,
hardness
bean
increased
9.79%
from
initial
period,
being
1.44
times
higher
than
HEER.
On
days
8
16
treated
decreased
24.38%
4.12%,
respectively.
Physiological
activity
analyses
indicated
treatment-induced
increase
was
associated
with
phenylpropanoid
metabolic
pathway.
Moreover,
significantly
down-regulated
expression
several
genes,
namely
FaPAL,
FaC4H,
FaCAD.
helps
deepen
understanding
inhibition
provide
new
insights
for
development
technology
bean.
Future Postharvest and Food,
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 14, 2024
Abstract
The
quantitative
determination
of
major
enzyme
activities
in
postharvest
fruit
is
a
necessary
biochemical
experiment
to
investigate
the
physiological
and
metabolic
mechanisms
ripeness,
senescence,
stress
responses.
However,
it
choose
appropriate
methods
stably
extract
determine
target
analytes
from
complex
plant
matrices
due
dynamic
changes
during
storage.
Here,
we
outline
sensitive
accurate
for
measuring
associated
with
key
processes
including
respiration,
reactive
oxygen
species
scavenging,
membrane
lipid
degradation,
energy
synthesis,
cell
walls
disease
resistance.
This
manuscript
aims
provide
guidance
future
research
biology.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(43), С. 23671 - 23688
Опубликована: Окт. 15, 2024
Most
postharvest
fruits
are
highly
perishable,
which
directly
impairs
fruit
taste
and
causes
an
economic
loss
of
fresh
products.
Thus,
it
is
necessary
to
find
effective
techniques
alleviate
this
issue.
Recently,
nitric
oxide
(NO)
brassinosteroid
(BR)
have
been
developed
as
alternatives
improve
quality.
This
work
mainly
reviews
the
recent
processes
NO
BR
in
improving
quality
during
postharvest.
Exogenous
or
treatments
delayed
senescence,
enhanced
disease
resistance,
alleviated
chilling
injury
fruit,
potential
physiological
biochemical
mechanisms
include
(1)
enhancing
antioxidant
defense
ability,
(2)
affecting
ethylene
biosynthesis,
(3)
regulating
sugar
energy
metabolism,
(4)
mediating
plant
hormone
signaling,
(5)
protein
S-nitrosylation
DNA
methylation.
review
concludes
functions
Additionally,
a
specific
finding
possible
crosstalk
applications
storage,
provides
new
insights
into
applicability
for
delaying
resistances
alleviating
fruit.
Journal of Food Science,
Год журнала:
2025,
Номер
90(2)
Опубликована: Фев. 1, 2025
Abstract
A
typical
symptom
of
post‐harvest
pineapple
(
Ananas
comosus
L.)
internal
browning
is
influenced
by
multiple
factors.
However,
the
precise
mechanism
through
which
fruit
size
influences
occurrence
remains
unclear.
Therefore,
this
study
aimed
to
investigate
impacts
in
and
its
possible
mechanisms
harvested
using
physical
biochemical
analysis
RNA‐seq.
Disease
incidence
was
assessed
four
groups
fruits
(1,
1.5,
2,
2.5
±
0.2
kg),
with
two
most
significant
(1
kg)
selected
for
detailed
analysis.
The
results
showed
that
large
had
faster
senescence
membrane
lipid
peroxidation,
higher
respiration
intensity,
more
reactive
oxygen
species
accumulation,
malondialdehyde
content.
Likewise,
lower
antioxidant
capacity
such
as
ascorbic
acid,
ascorbate
peroxidase,
catalase,
superoxide
dismutase
polyphenol
oxidase
activity,
peroxidase
phenylalanine
ammonia‐lyase
lipoxygenase
activity.
It
can
be
concluded
were
severely
subjected
stress
peroxidation
during
storage.
Gene
ontology
enrichment
reveals
relates
mainly
oxidoreductase
glutathione
metabolism.
accelerated
AsA–GSH
cycle
pathway
Real‐time
quantitative
PCR
shows
downregulated
expression
AcAPX1
,
AcAPX6
×
1
AcCAT2
AcSOD[Fe]2
AcSODX2
fruits,
whereas
AcPPO
AcLOX
upregulated
expression.
This
offers
insights
into
browning.
Future
molecular
techniques
may
reduce
