Convergent evolution of plant prickles by repeated gene co-option over deep time

Science, Год журнала: 2024, Номер 385(6708)

Опубликована: Авг. 1, 2024

An enduring question in evolutionary biology concerns the degree to which episodes of convergent trait evolution depend on same genetic programs, particularly over long timescales. In this work, we genetically dissected repeated origins and losses prickles-sharp epidermal projections-that convergently evolved numerous plant lineages. Mutations a cytokinin hormone biosynthetic gene caused at least 16 independent prickles eggplants wild relatives genus

Язык: Английский

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Convergent evolution of plant prickles is driven by repeated gene co-option over deep time

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 22, 2024

Abstract An enduring question in evolutionary biology concerns the degree to which episodes of convergent trait evolution depend on same genetic programs, particularly over long timescales. Here we genetically dissected repeated origins and losses prickles, sharp epidermal projections, that convergently evolved numerous plant lineages. Mutations a cytokinin hormone biosynthetic gene caused at least 16 independent prickles eggplants wild relatives genus Solanum . Strikingly, homologs promote prickle formation across angiosperms collectively diverged 150 million years ago. By developing new systems, leveraged this discovery eliminate species an indigenously foraged berry. Our findings implicate shared hormone-activation program underlying evolutionarily widespread recurrent instances morphological innovation.

Язык: Английский

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PE (Prickly Eggplant) encoding a cytokinin-activating enzyme responsible for the formation of prickles in eggplant

Horticulture Research, Год журнала: 2024, Номер 11(7)

Опубликована: Май 10, 2024

Abstract Eggplant is one of the most important vegetables worldwide, with some varieties displaying prickles. These prickles, present on leaves, stems, and fruit calyxes, posing challenges during cultivation, harvesting, transportation, making them an undesirable agronomic trait. However, genetic mechanisms underlying prickle morphogenesis in eggplant remain poorly understood, impeding improvements. In this study, analyses revealed that governed by a single dominant nuclear gene, termed PE (Prickly Eggplant). Subsequent bulk segregant RNA-sequencing (BSR-seq) linkage analysis preliminarily mapped to chromosome 6. This locus was then fine 9233 bp interval segregating population 1109 plants, harboring only candidate SmLOG1, which encodes LONELY GUY (LOG)-family cytokinin biosynthetic enzyme. Expression via transcriptome qRT-PCR demonstrate SmLOG1 predominantly expressed immature CRISPR-Cas9 knockout experiments targeting prickly parental line ‘PI 381159’ abolished prickles across all tissues, confirming its critical role morphogenesis. Sequence pinpointed variations solely within non-coding region. We developed cleaved amplified polymorphic sequences (CAPS) marker from distinct SNP located at −735-bp promoter, finding significant association variation 190 germplasms. findings enhance our understanding molecular governing development facilitate use marker-assisted selection (MAS) for breeding prickleless cultivars.

Язык: Английский

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Map-based cloning of LPD, a major gene positively regulates leaf prickle development in eggplant

Theoretical and Applied Genetics, Год журнала: 2024, Номер 137(10)

Опубликована: Сен. 9, 2024

Язык: Английский

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