LvbHLH13 Regulates Anthocyanin Biosynthesis by Activating the LvMYB5 Promoter in Lily (Lilium ‘Viviana’) DOI Creative Commons

Wenzhong An,

Yibo Sun,

Zhenhua Gao

и другие.

Horticulturae, Год журнала: 2024, Номер 10(9), С. 926 - 926

Опубликована: Авг. 30, 2024

Anthocyanins, constituents of flavonoid compounds prevalent in plants, possess significant value both plant development and human nutrition. The regulation anthocyanin biosynthesis primarily involves the orchestration MYB, bHLH, WD40 transcription factors. Consequently, bHLH family assumes a pivotal role modulating developmental processes. In present investigation, factor, denoted as LvbHLH13, was identified positive regulator pigmentation lily petals. LvbHLH13 is classified within IIId subgroup Arabidopsis proteins. Functional analyses involving transient expression gene silencing revealed its capacity to enhance diminish accumulation, respectively, by LvMYB5 expression, thereby influencing downstream structural expression. overexpression resulted an increase genes related synthesis, whereas correspondingly decreased Yeast one-hybrid EMSA assays demonstrated interaction between promoter, leading activation biosynthesis. A further luciferase (LUC) analysis corroborated stimulatory effect on promoter sequence. assumed crucial lily-petal pigmentation. yeast two-hybrid that diverged from typical factor behavior it did not form complex with MYB regulate This discrepancy could be attributed deletion N-terminal conserved sequence LvbHLH13. study provides new candidate bHLH-MYB partner explore regulatory network research opportunities for breeding lilies various contents. These findings lay theoretical foundation subsequent investigations into flower coloring mechanisms.

Язык: Английский

LvWRKY75 enhances the transcription of LvMYB5 and promotes anthocyanin biosynthesis in lily petals during the blooming phase DOI Open Access
Yibo Sun, X Zhang, Hao Zhang

и другие.

Physiologia Plantarum, Год журнала: 2025, Номер 177(2)

Опубликована: Март 1, 2025

Anthocyanin accumulation plays a crucial role in enhancing Lilium petal colouration; however, breeding efforts are hindered by our lack of understanding the complex molecular mechanism behind pigment's synthesis. This study explores potential WRKY family gene WRKY75 anthocyanin synthesis lilies. Contrary to inhibitory effect observed Arabidopsis thaliana, both transient silencing and overexpression analyses LvWRKY75 indicate that positively regulates The was found cause significant upregulation structural genes pivotal for biosynthesis lilies, including Lv3GT, LvDFR LvANS, as well regulatory LvMYB5. Further in-depth analyses, yeast one-hybrid, electrophoretic mobility shift assay, dual-luciferase assays, demonstrated binds promoter LvMYB5, its transcriptional activity. In turn, increased expression LvMYB5 upregulates transcription downstream such LvANS. summary, this provides deeper mechanisms contributing improving strategies flowers' ornamental value commercial appeal.

Язык: Английский

Процитировано

1
Light regulates SlCOP1-mediated degradation of SlJAF13, a transcription factor essential for anthocyanin biosynthesis in Aft tomato fruit DOI

Zhihong Cao,

Yunzhu Chen,

Yuansen Chen

и другие.

Plant Physiology and Biochemistry, Год журнала: 2025, Номер unknown, С. 109572 - 109572

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
LvbHLH13 Regulates Anthocyanin Biosynthesis by Activating the LvMYB5 Promoter in Lily (Lilium ‘Viviana’) DOI Creative Commons

Wenzhong An,

Yibo Sun,

Zhenhua Gao

и другие.

Horticulturae, Год журнала: 2024, Номер 10(9), С. 926 - 926

Опубликована: Авг. 30, 2024

Anthocyanins, constituents of flavonoid compounds prevalent in plants, possess significant value both plant development and human nutrition. The regulation anthocyanin biosynthesis primarily involves the orchestration MYB, bHLH, WD40 transcription factors. Consequently, bHLH family assumes a pivotal role modulating developmental processes. In present investigation, factor, denoted as LvbHLH13, was identified positive regulator pigmentation lily petals. LvbHLH13 is classified within IIId subgroup Arabidopsis proteins. Functional analyses involving transient expression gene silencing revealed its capacity to enhance diminish accumulation, respectively, by LvMYB5 expression, thereby influencing downstream structural expression. overexpression resulted an increase genes related synthesis, whereas correspondingly decreased Yeast one-hybrid EMSA assays demonstrated interaction between promoter, leading activation biosynthesis. A further luciferase (LUC) analysis corroborated stimulatory effect on promoter sequence. assumed crucial lily-petal pigmentation. yeast two-hybrid that diverged from typical factor behavior it did not form complex with MYB regulate This discrepancy could be attributed deletion N-terminal conserved sequence LvbHLH13. study provides new candidate bHLH-MYB partner explore regulatory network research opportunities for breeding lilies various contents. These findings lay theoretical foundation subsequent investigations into flower coloring mechanisms.

Язык: Английский

Процитировано

4