Light regulates SlCOP1-mediated degradation of SlJAF13, a transcription factor essential for anthocyanin biosynthesis in Aft tomato fruit
Plant Physiology and Biochemistry,
Год журнала:
2025,
Номер
unknown, С. 109572 - 109572
Опубликована: Янв. 1, 2025
Язык: Английский
LvWRKY75 enhances the transcription of LvMYB5 and promotes anthocyanin biosynthesis in lily petals during the blooming phase
Physiologia Plantarum,
Год журнала:
2025,
Номер
177(2)
Опубликована: Март 1, 2025
Anthocyanin
accumulation
plays
a
crucial
role
in
enhancing
Lilium
petal
colouration;
however,
breeding
efforts
are
hindered
by
our
lack
of
understanding
the
complex
molecular
mechanism
behind
pigment's
synthesis.
This
study
explores
potential
WRKY
family
gene
WRKY75
anthocyanin
synthesis
lilies.
Contrary
to
inhibitory
effect
observed
Arabidopsis
thaliana,
both
transient
silencing
and
overexpression
analyses
LvWRKY75
indicate
that
positively
regulates
The
was
found
cause
significant
upregulation
structural
genes
pivotal
for
biosynthesis
lilies,
including
Lv3GT,
LvDFR
LvANS,
as
well
regulatory
LvMYB5.
Further
in-depth
analyses,
yeast
one-hybrid,
electrophoretic
mobility
shift
assay,
dual-luciferase
assays,
demonstrated
binds
promoter
LvMYB5,
its
transcriptional
activity.
In
turn,
increased
expression
LvMYB5
upregulates
transcription
downstream
such
LvANS.
summary,
this
provides
deeper
mechanisms
contributing
improving
strategies
flowers'
ornamental
value
commercial
appeal.
Язык: Английский
LvbHLH13 Regulates Anthocyanin Biosynthesis by Activating the LvMYB5 Promoter in Lily (Lilium ‘Viviana’)
Wenzhong An,
Yibo Sun,
Zhenhua Gao
и другие.
Horticulturae,
Год журнала:
2024,
Номер
10(9), С. 926 - 926
Опубликована: Авг. 30, 2024
Anthocyanins,
constituents
of
flavonoid
compounds
prevalent
in
plants,
possess
significant
value
both
plant
development
and
human
nutrition.
The
regulation
anthocyanin
biosynthesis
primarily
involves
the
orchestration
MYB,
bHLH,
WD40
transcription
factors.
Consequently,
bHLH
family
assumes
a
pivotal
role
modulating
developmental
processes.
In
present
investigation,
factor,
denoted
as
LvbHLH13,
was
identified
positive
regulator
pigmentation
lily
petals.
LvbHLH13
is
classified
within
IIId
subgroup
Arabidopsis
proteins.
Functional
analyses
involving
transient
expression
gene
silencing
revealed
its
capacity
to
enhance
diminish
accumulation,
respectively,
by
LvMYB5
expression,
thereby
influencing
downstream
structural
expression.
overexpression
resulted
an
increase
genes
related
synthesis,
whereas
correspondingly
decreased
Yeast
one-hybrid
EMSA
assays
demonstrated
interaction
between
promoter,
leading
activation
biosynthesis.
A
further
luciferase
(LUC)
analysis
corroborated
stimulatory
effect
on
promoter
sequence.
assumed
crucial
lily-petal
pigmentation.
yeast
two-hybrid
that
diverged
from
typical
factor
behavior
it
did
not
form
complex
with
MYB
regulate
This
discrepancy
could
be
attributed
deletion
N-terminal
conserved
sequence
LvbHLH13.
study
provides
new
candidate
bHLH-MYB
partner
explore
regulatory
network
research
opportunities
for
breeding
lilies
various
contents.
These
findings
lay
theoretical
foundation
subsequent
investigations
into
flower
coloring
mechanisms.
Язык: Английский