Sucrose Promotes the Proliferation and Differentiation of Callus by Regulating ROS Intensity in Agapanthus praecox DOI Creative Commons
Jianhua Yue, Yan Dong,

Changmei Du

и другие.

Horticulturae, Год журнала: 2024, Номер 10(12), С. 1350 - 1350

Опубликована: Дек. 16, 2024

The proliferation and differentiation of callus is the foundation for plant regeneration propagation. type carbon sources in medium significantly influences efficacy plants vitro. Our study performed transcriptomic physiological analyses utilizing sucrose, glucose, maltose to understand molecular characteristics potential affected by Agapanthus praecox. Differentially expressed genes were notably associated with hormone signal transduction, glycolysis/gluconeogenesis, MAPK signaling callus. indicators suggest glucose enhanced both cell size increasing endogenous indole-3-acetic acid (IAA), cytokinin, brassinosteroid, gibberellin (GAs), starch, levels, while concurrently reducing levels reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) hydroxyl radical (·OH). Conversely, sucrose treatment promoted elevating IAA oxidase activity alongside stress-related hormones abscisic ethylene levels. Additionally, led increased accumulation fructose, H2O2, ·OH within tissue. Furthermore, influenced regenerative capacity modulating glycometabolism osmoregulation. posits that facilitates via diminished ROS intensity promotes maintaining moderate Altogether, our results capabilities regulating A.

Язык: Английский

RBOH-dependent signaling is involved in He-Ne laser-induced salt tolerance and production of rosmarinic acid and carnosol in Salvia officinalis DOI Creative Commons

Fatemeh Mardani-Korrani,

Rayhaneh Amooaghaie, Ali Mohammad Ahadi

и другие.

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Авг. 24, 2024

In the past two decades, impacts of Helium-Neon (He–Ne) laser on stress resistance and secondary metabolism in plants have been studied, but signaling pathway which by regulates this process remains unclear. Therefore, current study sought to explore role RBOH-dependent He–Ne laser-induced salt tolerance elicitation Salvia officinalis. Seeds were primed with (6 J cm− 2) peroxide hydrogen (H2O2, 5 mM) 15-old-day exposed salinity levels (0, 75 mM NaCl). Salt reduced growth parameters, chlorophyll content relative water (RWC) increased malodialdehyde (MDA) H2O2 contents leaves 45-old-day plants. After 48 h exposure, higher transcription RBOH (encoding NADPH oxidase), PAL (phenylalanine ammonia-lyase), RAS (rosmarinic acid synthase) recorded grown from seeds and/or H2O2. Despite up-regulated gene early hours exposing salinity, MDA lower these after 30 days. Seed pretreatment augmented accumulation anthocyanins, total phenol, carnasol, rosmarinic antioxidant capacity under non-saline more extensively at saline conditions. Indeed, treatments improved RWC, K+/Na+ ratio, enhanced activities superoxide dismutase ascorbate peroxidase proline accumulation, significantly decreased membrane injury stress. However, applying diphenylene iodonium (DPI as an inhibitor oxidase) N, N-dimethyl thiourea (DMTU a scavenger) priming reversed aforementioned effects turn resulted loss metabolism. These findings for first time deciphered that can induce transient burst, might act downstream signal promote alleviation S. officinalis

Язык: Английский

Процитировано

5

Seed laser priming enhances defensive responses in milk thistle under Pb toxicity DOI Creative Commons

Atefeh Banisharif,

Rayhaneh Amooaghaie

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 6, 2025

Heavy metal stress negatively affects the growth of medicinal plants. While effects Helium-Neon (He-Ne) laser on seed germination and tolerance in plants has garnered significant attention, little is known concerning impacts He-Ne irradiation heavy Therefore, current study was conducted to appraise effect different durations (0, 20, 40 min) priming with (10 mW mm-2) antioxidant system Silybum marianum L. under various Pb concentrations 250, 500 ppm). Lead phytotoxicity evident by reductions fresh dry weights shoots roots, total chlorophyll (TChl) content relative water (RWC), as well increases H2O2 malondialdehyde contents roots leaves. Seed for 20 min significantly improved these parameters, enhancing tolerance. Conversely, prolonged (40 resulted less favorable outcomes, including reduced growth, TChl content, RWC, while also exacerbating oxidative damage membranes even non-stressful conditions. The 20-min systemically mitigated Pb-induced lipid peroxidation accumulation boosting activities superoxide dismutase catalase increasing proline leaves milk thistle These findings multivariate analysis suggest that optimal dose initiates a "stress memory" seeds which activated upon subsequent exposure stress, plant defensive mechanisms enabling better cope damage. This underscore promising potential novel strategy like thistle, offering an eco-friendly technique maintaining their productivity stress.

Язык: Английский

Процитировано

0

Silicon Nanoparticles and Apoplastic Protein Interaction: A Hypothesized Mechanism for Modulating Plant Growth and Immunity DOI Creative Commons
Guopeng Miao, Juan Han,

Taotao Han

и другие.

Plants, Год журнала: 2025, Номер 14(11), С. 1630 - 1630

Опубликована: Май 27, 2025

Silicon nanoparticles (SiNPs) have emerged as multifunctional tools in sustainable agriculture, demonstrating significant efficacy promoting crop growth and enhancing plant resilience against diverse biotic abiotic stresses. Although their ability to strengthen antioxidant defense systems activate systemic immune responses is well documented, the fundamental mechanisms driving these benefits remain unclear. This review synthesizes emerging evidence propose an innovative paradigm: SiNPs remodel redox signaling networks stress adaptation by forming protein coronas through apoplastic adsorption. We hypothesize that extracellular may elevate reactive oxygen species (ROS) levels adsorbing inhibiting enzymes, thereby intracellular buffering capacity activating salicylic acid (SA)-dependent pathways. Conversely, smaller infiltrating symplastic compartments risk oxidative damage due direct suppression of cytoplasmic systems. Additionally, indirectly influence heavy metal transporter activity state regulation broadly modulate physiological functions via transcription factor regulatory networks. Critical knowledge gaps persist regarding dynamic composition under varying environmental conditions transgenerational impacts. By integrating existing SiNPs, this provides insights potential strategies for developing novel agrochemicals stress-resistant crops.

Язык: Английский

Процитировано

0

Sucrose Promotes the Proliferation and Differentiation of Callus by Regulating ROS Intensity in Agapanthus praecox DOI Creative Commons
Jianhua Yue, Yan Dong,

Changmei Du

и другие.

Horticulturae, Год журнала: 2024, Номер 10(12), С. 1350 - 1350

Опубликована: Дек. 16, 2024

The proliferation and differentiation of callus is the foundation for plant regeneration propagation. type carbon sources in medium significantly influences efficacy plants vitro. Our study performed transcriptomic physiological analyses utilizing sucrose, glucose, maltose to understand molecular characteristics potential affected by Agapanthus praecox. Differentially expressed genes were notably associated with hormone signal transduction, glycolysis/gluconeogenesis, MAPK signaling callus. indicators suggest glucose enhanced both cell size increasing endogenous indole-3-acetic acid (IAA), cytokinin, brassinosteroid, gibberellin (GAs), starch, levels, while concurrently reducing levels reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) hydroxyl radical (·OH). Conversely, sucrose treatment promoted elevating IAA oxidase activity alongside stress-related hormones abscisic ethylene levels. Additionally, led increased accumulation fructose, H2O2, ·OH within tissue. Furthermore, influenced regenerative capacity modulating glycometabolism osmoregulation. posits that facilitates via diminished ROS intensity promotes maintaining moderate Altogether, our results capabilities regulating A.

Язык: Английский

Процитировано

0