Seed laser priming enhances defensive responses in milk thistle under Pb toxicity
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Март 6, 2025
Heavy
metal
stress
negatively
affects
the
growth
of
medicinal
plants.
While
effects
Helium-Neon
(He-Ne)
laser
on
seed
germination
and
tolerance
in
plants
has
garnered
significant
attention,
little
is
known
concerning
impacts
He-Ne
irradiation
heavy
Therefore,
current
study
was
conducted
to
appraise
effect
different
durations
(0,
20,
40
min)
priming
with
(10
mW
mm-2)
antioxidant
system
Silybum
marianum
L.
under
various
Pb
concentrations
250,
500
ppm).
Lead
phytotoxicity
evident
by
reductions
fresh
dry
weights
shoots
roots,
total
chlorophyll
(TChl)
content
relative
water
(RWC),
as
well
increases
H2O2
malondialdehyde
contents
roots
leaves.
Seed
for
20
min
significantly
improved
these
parameters,
enhancing
tolerance.
Conversely,
prolonged
(40
resulted
less
favorable
outcomes,
including
reduced
growth,
TChl
content,
RWC,
while
also
exacerbating
oxidative
damage
membranes
even
non-stressful
conditions.
The
20-min
systemically
mitigated
Pb-induced
lipid
peroxidation
accumulation
boosting
activities
superoxide
dismutase
catalase
increasing
proline
leaves
milk
thistle
These
findings
multivariate
analysis
suggest
that
optimal
dose
initiates
a
"stress
memory"
seeds
which
activated
upon
subsequent
exposure
stress,
plant
defensive
mechanisms
enabling
better
cope
damage.
This
underscore
promising
potential
novel
strategy
like
thistle,
offering
an
eco-friendly
technique
maintaining
their
productivity
stress.
Язык: Английский
RBOH-dependent signaling is involved in He-Ne laser-induced salt tolerance and production of rosmarinic acid and carnosol in Salvia officinalis
BMC Plant Biology,
Год журнала:
2024,
Номер
24(1)
Опубликована: Авг. 24, 2024
In
the
past
two
decades,
impacts
of
Helium-Neon
(He–Ne)
laser
on
stress
resistance
and
secondary
metabolism
in
plants
have
been
studied,
but
signaling
pathway
which
by
regulates
this
process
remains
unclear.
Therefore,
current
study
sought
to
explore
role
RBOH-dependent
He–Ne
laser-induced
salt
tolerance
elicitation
Salvia
officinalis.
Seeds
were
primed
with
(6
J
cm−
2)
peroxide
hydrogen
(H2O2,
5
mM)
15-old-day
exposed
salinity
levels
(0,
75
mM
NaCl).
Salt
reduced
growth
parameters,
chlorophyll
content
relative
water
(RWC)
increased
malodialdehyde
(MDA)
H2O2
contents
leaves
45-old-day
plants.
After
48
h
exposure,
higher
transcription
RBOH
(encoding
NADPH
oxidase),
PAL
(phenylalanine
ammonia-lyase),
RAS
(rosmarinic
acid
synthase)
recorded
grown
from
seeds
and/or
H2O2.
Despite
up-regulated
gene
early
hours
exposing
salinity,
MDA
lower
these
after
30
days.
Seed
pretreatment
augmented
accumulation
anthocyanins,
total
phenol,
carnasol,
rosmarinic
antioxidant
capacity
under
non-saline
more
extensively
at
saline
conditions.
Indeed,
treatments
improved
RWC,
K+/Na+
ratio,
enhanced
activities
superoxide
dismutase
ascorbate
peroxidase
proline
accumulation,
significantly
decreased
membrane
injury
stress.
However,
applying
diphenylene
iodonium
(DPI
as
an
inhibitor
oxidase)
N,
N-dimethyl
thiourea
(DMTU
a
scavenger)
priming
reversed
aforementioned
effects
turn
resulted
loss
metabolism.
These
findings
for
first
time
deciphered
that
can
induce
transient
burst,
might
act
downstream
signal
promote
alleviation
S.
officinalis
Язык: Английский
Silicon Nanoparticles and Apoplastic Protein Interaction: A Hypothesized Mechanism for Modulating Plant Growth and Immunity
Plants,
Год журнала:
2025,
Номер
14(11), С. 1630 - 1630
Опубликована: Май 27, 2025
Silicon
nanoparticles
(SiNPs)
have
emerged
as
multifunctional
tools
in
sustainable
agriculture,
demonstrating
significant
efficacy
promoting
crop
growth
and
enhancing
plant
resilience
against
diverse
biotic
abiotic
stresses.
Although
their
ability
to
strengthen
antioxidant
defense
systems
activate
systemic
immune
responses
is
well
documented,
the
fundamental
mechanisms
driving
these
benefits
remain
unclear.
This
review
synthesizes
emerging
evidence
propose
an
innovative
paradigm:
SiNPs
remodel
redox
signaling
networks
stress
adaptation
by
forming
protein
coronas
through
apoplastic
adsorption.
We
hypothesize
that
extracellular
may
elevate
reactive
oxygen
species
(ROS)
levels
adsorbing
inhibiting
enzymes,
thereby
intracellular
buffering
capacity
activating
salicylic
acid
(SA)-dependent
pathways.
Conversely,
smaller
infiltrating
symplastic
compartments
risk
oxidative
damage
due
direct
suppression
of
cytoplasmic
systems.
Additionally,
indirectly
influence
heavy
metal
transporter
activity
state
regulation
broadly
modulate
physiological
functions
via
transcription
factor
regulatory
networks.
Critical
knowledge
gaps
persist
regarding
dynamic
composition
under
varying
environmental
conditions
transgenerational
impacts.
By
integrating
existing
SiNPs,
this
provides
insights
potential
strategies
for
developing
novel
agrochemicals
stress-resistant
crops.
Язык: Английский
Sucrose Promotes the Proliferation and Differentiation of Callus by Regulating ROS Intensity in Agapanthus praecox
Horticulturae,
Год журнала:
2024,
Номер
10(12), С. 1350 - 1350
Опубликована: Дек. 16, 2024
The
proliferation
and
differentiation
of
callus
is
the
foundation
for
plant
regeneration
propagation.
type
carbon
sources
in
medium
significantly
influences
efficacy
plants
vitro.
Our
study
performed
transcriptomic
physiological
analyses
utilizing
sucrose,
glucose,
maltose
to
understand
molecular
characteristics
potential
affected
by
Agapanthus
praecox.
Differentially
expressed
genes
were
notably
associated
with
hormone
signal
transduction,
glycolysis/gluconeogenesis,
MAPK
signaling
callus.
indicators
suggest
glucose
enhanced
both
cell
size
increasing
endogenous
indole-3-acetic
acid
(IAA),
cytokinin,
brassinosteroid,
gibberellin
(GAs),
starch,
levels,
while
concurrently
reducing
levels
reactive
oxygen
species
(ROS)
such
as
hydrogen
peroxide
(H2O2)
hydroxyl
radical
(·OH).
Conversely,
sucrose
treatment
promoted
elevating
IAA
oxidase
activity
alongside
stress-related
hormones
abscisic
ethylene
levels.
Additionally,
led
increased
accumulation
fructose,
H2O2,
·OH
within
tissue.
Furthermore,
influenced
regenerative
capacity
modulating
glycometabolism
osmoregulation.
posits
that
facilitates
via
diminished
ROS
intensity
promotes
maintaining
moderate
Altogether,
our
results
capabilities
regulating
A.
Язык: Английский