Identification and Structural Elucidation of Acylsugars in Tomato Leaves Using Liquid Chromatography–Ion Mobility–Tandem Mass Spectrometry (LC-IM-MS/MS) DOI
Kimberly Y. Kartowikromo, Jéssica S. Pizzo, Thiago Rutz

и другие.

Journal of the American Society for Mass Spectrometry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 16, 2024

Leaves of tomato plants contain various glandular trichomes that produce a wide range metabolic products including acylsugars, which may serve as defense mechanism against insect pests. Acylsugars exhibit significant structural diversity, differing in their sugar cores, acylated positions, and type acyl chains. This work demonstrated comprehensive approach using multidimensional separation techniques, specifically liquid chromatography–ion mobility–tandem mass spectrometry (LC-IM-MS/MS), for characterization, the discrimination different (one cultivar five accessions) was leaf extracts; six genotypes from species Solanum were represented. As result, we identified 16 acylsugars through molecular formulas annotations LC MS analyses. The incorporation ion mobility (IM) analysis revealed an additional 9 isomeric forms, resulting total 25 identified. Furthermore, experimental collision cross section (CCSexp) values agreed reasonably well with corresponding predicted (CCSpred), overall estimated error less than 2%. These findings pave way research into how isomers might influence self-defense plants. Moreover, this investigated accessions tomatoes can be distinguished each other based on metabolite profile, e.g., principal component (PCA) linear discriminant (LDA) statistical models, yielding prediction rate 98.3%.

Язык: Английский

Identification and Structural Elucidation of Acylsugars in Tomato Leaves Using Liquid Chromatography–Ion Mobility–Tandem Mass Spectrometry (LC-IM-MS/MS) DOI
Kimberly Y. Kartowikromo, Jéssica S. Pizzo, Thiago Rutz

и другие.

Journal of the American Society for Mass Spectrometry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 16, 2024

Leaves of tomato plants contain various glandular trichomes that produce a wide range metabolic products including acylsugars, which may serve as defense mechanism against insect pests. Acylsugars exhibit significant structural diversity, differing in their sugar cores, acylated positions, and type acyl chains. This work demonstrated comprehensive approach using multidimensional separation techniques, specifically liquid chromatography–ion mobility–tandem mass spectrometry (LC-IM-MS/MS), for characterization, the discrimination different (one cultivar five accessions) was leaf extracts; six genotypes from species Solanum were represented. As result, we identified 16 acylsugars through molecular formulas annotations LC MS analyses. The incorporation ion mobility (IM) analysis revealed an additional 9 isomeric forms, resulting total 25 identified. Furthermore, experimental collision cross section (CCSexp) values agreed reasonably well with corresponding predicted (CCSpred), overall estimated error less than 2%. These findings pave way research into how isomers might influence self-defense plants. Moreover, this investigated accessions tomatoes can be distinguished each other based on metabolite profile, e.g., principal component (PCA) linear discriminant (LDA) statistical models, yielding prediction rate 98.3%.

Язык: Английский

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