The toxicity potential of pharmaceuticals found in the Douro River estuary (Portugal): Assessing impacts on gonadal maturation with a histopathological and stereological study of zebrafish ovary and testis after sub-acute exposures

Aquatic Toxicology, Год журнала: 2011, Номер 105(3-4), С. 292 - 299

Опубликована: Июнь 30, 2011

Язык: Английский

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Assessment of pollution in sewage ponds using biomarker responses in wild African sharptooth catfish (Clarias gariepinus) in Tanzania

Ecotoxicology, Год журнала: 2009, Номер 19(4), С. 722 - 734

Опубликована: Дек. 10, 2009

Язык: Английский

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Histopathological changes in Oreochromis mossambicus (Peters, 1852) ovaries after a chronic exposure to a mixture of the HIV drug nevirapine and the antibiotics sulfamethoxazole and trimethoprim

Chemosphere, Год журнала: 2021, Номер 274, С. 129900 - 129900

Опубликована: Фев. 10, 2021

The burden of the human immunodeficiency virus and acquired syndrome (HIV/AIDS) infection has transformed African continent into a major consumer antiretrovirals (ARVs) drugs. In addition to HIV burden, also high incidence tuberculosis (TB) been experiencing recurring outbreaks several other viral, bacterial, parasitic epidemic diseases. novel severe acute respiratory coronavirus 2 (SARS-COV-2 or Covid-19) pandemic outbreak is adding continent's infectious diseases as experts are predicting that it will be here for long time. One consequences these antiviral antibiotic compounds have become some most consumed pharmaceuticals on continent. Many drugs frequently detected in surface waters across Africa. There limited information available adverse effects mixtures different types aquatic environments fish reproduction. present study investigated ARV drug nevirapine (NVP - 1.48 3.74 μg/L) its mixture with sulfamethoxazole (3.68 trimethoprim (0.87 O. mossambicus gonads using histopathological endpoints biomarkers. (n = 52) were exposed 30 days static renewal system. Female (3.74 NVP – recorded higher ovary indices. Statistically significant differences found female indices between control (p 0.002) well 0.009). main observed changes ovaries increased vitellogenic oocyte atresia vacuolation interstitial tissue mixture. It evident presence antibiotics water triggered histopathology ovaries. abnormal rate atretic oocytes could result impaired

Язык: Английский

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An enzyme-linked immunosorbent assay for rare minnow (Gobiocypris rarus) vitellogenin and comparison of vitellogenin responses in rare minnow and zebrafish (Danio rerio)

The Science of The Total Environment, Год журнала: 2006, Номер 364(1-3), С. 284 - 294

Опубликована: Март 8, 2006

Язык: Английский

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Screening complex effluents for estrogenic activity with the T47D‐KBluc cell bioassay: Assay optimization and comparison with in vivo responses in fish

Environmental Toxicology and Chemistry, Год журнала: 2010, Номер 30(2), С. 439 - 445

Опубликована: Окт. 29, 2010

Abstract Wastewater treatment plant (WWTP) effluents can contain estrogenic chemicals, which potentially disrupt fish reproduction and development. The current study focused on the use of an estrogen‐responsive in vitro cell bioassay (T47D‐KBluc), to quantify total estrogenicity WWTP effluents. We tested a novel sample preparation method for T47D‐KBluc assay, using powdered media prepared with direct effluent. Results assay were compared induction estrogen receptor–regulated gene transcription male fathead minnows ( Pimephales promelas ) exposed same Effluent samples paired studies collected over course three months. According effluent ranged from 1.13 2.00 ng 17β‐estradiol (E2) equivalents/L. Corresponding vivo exposing 0, 10, 50, 100% dilutions demonstrated that exposure significantly increased hepatic vitellogenin (VTG) receptor α subunit transcripts relative controls. was also significant males 250 E2/L or 100 E2/L. results support conclusion contains activity, but there discrepancy between vitro– vivo–based E2 equivalent estimates. Our suggest is reasonable approach estimate wastewater Environ. Toxicol. Chem. 2011;30:439–445. © 2010 SETAC

Язык: Английский

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Gill histopathological alterations in Nile tilapia, Oreochromis niloticus exposed to treated sewage Water

Brazilian Archives of Biology and Technology, Год журнала: 2008, Номер 51(5), С. 1057 - 1063

Опубликована: Окт. 1, 2008

Adult Nile tilapia, Oreochromis niloticus, of both sexes were exposed in wastewater from a sewage treatment plant for period 4 days. Gill samples collected after 24, 48, 72 and 96 h histopathological changes analyzed by light scanning electronic microscopy. epithelium control O. niloticus (freshwater group) was similar to that other teleosts, while lesions observed fishes. The main edema, lifting lamellar filamentar epithelia fusion. Cell proliferation with consequent thickening the filament also found fishes treated water. severity increased time exposure, namely hyperplasia epithelial cells fusion lamellae at h. Additionally, several results obtained microscopy confirmed through

Язык: Английский

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Endocrine disruption in Sphoeroides testudineus tissues and sediments highlights contamination in a northeastern Brazilian estuary

Environmental Monitoring and Assessment, Год журнала: 2016, Номер 188(5)

Опубликована: Апрель 19, 2016

Язык: Английский

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Biochemical and histological alterations in Aequidens metae (Pisces, Cichlidae) and Astyanax gr. bimaculatus (Pisces, Characidae) as indicators of river pollution

The Science of The Total Environment, Год журнала: 2019, Номер 692, С. 1234 - 1241

Опубликована: Июль 13, 2019

Язык: Английский

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Correlations of nonylphenol-ethoxylates and nonylphenol with biomarkers of reproductive function in carp (Cyprinus carpio) from the Cuyahoga River

The Science of The Total Environment, Год журнала: 2006, Номер 371(1-3), С. 391 - 401

Опубликована: Окт. 20, 2006

Язык: Английский

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Extraction and purification procedures for simultaneous quantification of phenolic xenoestrogens and steroid estrogens in river sediment by gas chromatography/ion trap mass spectrometry

Rapid Communications in Mass Spectrometry, Год журнала: 2008, Номер 22(22), С. 3651 - 3661

Опубликована: Окт. 22, 2008

Abstract A sensitive and simple method based on ultrasonication extraction with a hexane/acetone (2:1, v/v) mixture, followed by clean up of the extract solid‐phase (SPE) gas chromatography/mass spectrometry (GC/MS) detection, has been developed validated for analysis 20 estrogenic endocrine‐disrupting chemicals (EEDCs) including phenolic xenoestrogens, synthetic natural estrogens in river sediment. After purification, analytes are derivatised BSTFA/TMCS/pyridine (49:1:50, v/v/v) mixture quantified GC/MS. The GC/MS involves switching between electron ionisation (EI) chemical (CI); it also switches selected ion storage tandem mass detection. applicability demonstrated analysing extracts French sediments which bioanalytical tests ( vitro ) had already shown that they were impacted endocrine disrupters. biological contribution all products detected each sediment was compared to activity measured bioassays. Copyright © 2008 John Wiley & Sons, Ltd.

Язык: Английский

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