Urban and agricultural land use led to niche differentiation of AOA, AOB and comammox along the Beiyun River continuum

Water Research, Год журнала: 2024, Номер 255, С. 121480 - 121480

Опубликована: Март 16, 2024

Язык: Английский

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Unraveling the important role of comammox Nitrospira to nitrification in the coastal aquaculture system

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Апрель 23, 2024

Increasing nitrogen (N) input to coastal ecosystems poses a serious environmental threat. It is important understand the responses and feedback of N removal microbial communities, particularly nitrifiers including newly recognized complete ammonia-oxidizers (comammox), improve aquaculture sustainability. In this study, we conducted holistic evaluation functional communities responsible for nitrification by quantifying sequencing key genes comammox Nitrospira-amoA, AOA-amoA, AOB-amoA Nitrospira-nxrB in fish ponds with different feeding levels evaluated contribution process through experiments mixing pure cultures. We found that higher dramatically increased N-related concentration, affecting nitrifying communities. Compared AOA AOB, Nitrospira NOB were more sensitive changes. Unexpectedly, detected an equivalent abundance AOB observed increase proportion clade A feeding. Furthermore, simplified network shift keystone species from fish-feeding ponds. Random forest analysis suggested community played critical role eutrophic (40–70 μM). Through additional experiment cultures, primary contributor at 200 μM ammonium. These results advance our understanding highlight importance driving systems.

Язык: Английский

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Ammonia-oxidizing archaea are dominant over comammox in soil nitrification under long-term nitrogen fertilization

Journal of Soils and Sediments, Год журнала: 2021, Номер 21(4), С. 1800 - 1814

Опубликована: Фев. 13, 2021

Язык: Английский

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Comammox Nitrospira Clade B is the most abundant complete ammonia oxidizer in a dairy pasture soil and inhibited by dicyandiamide and high ammonium concentrations

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Дек. 12, 2022

The recent discovery of comammox Nitrospira , a complete ammonia oxidizer, capable completing the nitrification on their own has presented tremendous challenges to our understanding process. There are two divergent clades Clade A and B. However, population abundance, community structure role in nitrite oxidation poorly understood. We conducted 94-day microcosm study using grazed dairy pasture soil amended with urea fertilizers, synthetic cow urine, inhibitor, dicyandiamide (DCD), investigate growth spp. discovered that B was orders magnitude more abundant than this fertile most subcluster distinctive phylogenetic uncultured B2. found might not play major compared canonical nitrite-oxidizers, however, is active inhibited by high ammonium concentration (700 kg urine-N ha –1 ) inhibitor DCD. concluded B: (1) soil; (2) had low tolerance can be DCD; (3) dominant nitrite-oxidizer soil. This first discovering new B2 from an agricultural

Язык: Английский

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Diversity distribution and characteristics of comammox in different ecosystems

Environmental Research, Год журнала: 2022, Номер 214, С. 113900 - 113900

Опубликована: Июль 15, 2022

Язык: Английский

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