Analytical and Bioanalytical Chemistry,
Год журнала:
2022,
Номер
414(19), С. 5877 - 5886
Опубликована: Июнь 4, 2022
Non-steroidal
anti-inflammatory
drugs
(NSAIDs)
have
been
reported
to
be
the
main
pharmaceutical
class
accumulated
in
seafood.
Among
them,
ibuprofen
(IBU)
is
of
special
concern
as
it
used
worldwide
treat
common
pain,
does
not
require
a
medical
prescription,
often
taken
high
daily
dose,
and
has
cause
potential
adverse
effects
on
aquatic
organisms.
IBU
highly
transformed
into
hydroxy-
carboxy-metabolites
and/or
degradation
products
generated
only
after
its
administration
but
also
during
wastewater
treatment
or
environment.
These
compounds
can
present
environment
at
higher
concentrations
than
toxicity.
In
this
work,
low-cost
affordable
routine
analytical
method
was
developed
validated
for
first-time
determination
metabolites
mussels.
The
based
ultrasound-assisted
extraction
(UAE),
clean-up
by
dispersive
solid-phase
(d-SPE)
liquid
chromatography-tandem
mass
spectrometry.
Box-Behnken
experimental
design
optimisation
better
evaluate
influence
interactions
UAE
d-SPE
variables.
Extraction
recoveries
were
range
from
81
115%.
Precision,
expressed
relative
standard
deviation,
lower
7%.
Method
detection
limits
0.1
1.9
ng
g-1
dry
weight.
successfully
applied
wild
Environmental Pollution,
Год журнала:
2024,
Номер
363, С. 125135 - 125135
Опубликована: Окт. 18, 2024
Nowadays,
aquatic
species
face
a
variety
of
environmental
risks
associated
with
pharmaceutical
consumption.
More
specifically,
the
increased
number
cancer
patients
has
been
accompanied
by
an
consumption
antineoplastic
drugs,
such
as
ifosfamide
(IF)
and
cyclophosphamide
(CP).
These
drugs
have
found
in
ecosystems,
raising
concerns
about
their
impact,
especially
on
estuarine
species,
marine
waters
are
final
recipients
continental
effluents.
Simultaneously,
predicted
climatic
changes,
salinity
shifts,
may
threaten
organisms.
Considering
this,
present
research
aims
to
investigate
combined
effects
IF
CP,
shifts.
For
transcriptomic,
biochemical,
histopathological
assessment
was
made
using
bivalve
Mytilus
galloprovincialis
exposed
for
28
days
CP
(500
ng/L),
individually,
at
different
levels
(20,
30,
40).
up-regulated
metabolism-related
gene
cyp3a1,
also
affecting
abcc
gene,
showing
minimal
impact
highlighting
importance
these
metabolic
routes
mussels.
Salinity
shifts
affected
transcription
genes
related
apoptosis
cell
cycle
growth,
p53,
well
aerobic
metabolism,
antioxidant
biotransformation
mechanisms.
findings
indicate
mussels'
high
adaptability
osmotic
stress.
Under
exposure
low
salinity,
mussels
exhibited
cellular
damage
digestive
gland
tubules,
revealing
detrimental
towards
M.
galloprovincialis,
suggesting
that
slowdown
activation
mechanisms
helped
prevent
oxidative
control
salinities.
Overall,
results
reinforce
need
antineoplastics
ecotoxicological
risk
assessment,
under
foreseen
climate
change
scenarios.
Journal of Xenobiotics,
Год журнала:
2024,
Номер
14(4), С. 2015 - 2038
Опубликована: Дек. 18, 2024
The
growing
use
of
products
containing
rare
earth
elements
(REEs)
may
lead
to
higher
environmental
emissions
these
elements,
which
can
potentially
enter
aquatic
systems.
Praseodymium
(Pr)
and
europium
(Eu)
are
widely
used
REEs
with
various
applications.
However,
their
ecotoxicological
impacts
remain
largely
unexplored,
poorly
understood
risks
wildlife.
Moreover,
organisms
also
face
stressors
like
salinity
fluctuations,
the
nature
interaction
between
variations
contaminants
is
not
yet
clear.
Therefore,
this
study
aimed
evaluate
influence
shifts
on
Pr
Eu
adult
mussels
sperm
species
Mytilus
galloprovincialis
after
28
days
30
min
exposure,
respectively.
To
do
so,
biochemical
histopathological
alterations
were
evaluated
in
adults,
while
physiological
changes
analysed
sperm.
Additionally,
Integrated
Biological
Index
(IBR)
was
calculated
understand
overall
impact
each
treatment.
results
showed
that
most
affected
when
exposed
combination
high
element,
altered
behaviour
defence
mechanisms
causing
redox
imbalance
cellular
damage.
On
other
hand,
demonstrated
sensitivity
specific
REE-salinity
combinations,
particularly
at
lower
salinity.
These
treatments
elicited
motility
velocity:
20
led
a
production
O2−
decrease
velocity,
40
resulted
reduced
an
increase
irregular
movement.
At
both
levels,
exposure
caused
similar
sensitivities
adults
sperm,
reflected
by
comparable
IBR
scores.
In
contrast,
induced
greater
than
salinity,
whereas
reverse
observed
findings
suggest
reproductive
success
population
dynamics
could
be
modulated
interactions
levels
REE
pollution,
highlighting
need
for
further
investigation
into
how
factors
interact.
This
offers
valuable
insights
inform
policymakers
about
potential
contamination,
emphasising
importance
implementing
regulations
developing
strategies
mitigate
pollutants.
The Science of The Total Environment,
Год журнала:
2024,
Номер
959, С. 177995 - 177995
Опубликована: Дек. 24, 2024
Neodymium
(Nd)
and
dysprosium
(Dy)
are
among
the
most
commonly
used
rare
earth
elements
(REEs)
worldwide,
leading
to
their
increased
concentrations
in
aquatic
environments,
with
potential
impacts
on
organisms.
Additionally,
organisms
also
subjected
environmental
stressors,
such
as
salinity
shifts,
which
can
not
only
directly
impact
health
but
modulate
of
contaminants.
Therefore,
this
study
aimed
evaluate
effects
Nd
Dy
adult
mussels
sperm
species
Mytilus
galloprovincialis
after
28
days
30
min
exposure,
respectively.
Furthermore,
assessed
how
shifts
influence
these
elements.
To
end,
histopathological
biochemical
alterations
were
evaluated
adults,
while
physiological
changes
analyzed
sperm.
simplify
data
interpretation
understand
overall
each
treatment,
Multidimensional
Scaling
Analysis
(MDS)
Integrated
Biological
Index
(IBR)
used.
The
MDS
analysis
identified
three
groups
that
consistent
IBR
score.
first
group,
composed
treatments
at
a
40,
showed
medium
severe
effects.
second
group
was
comprised
control
(30),
presented
lowest
scores,
indicating
least
impact.
third
included
20,
associated
impacts,
presenting
highest
scores.
results
predominant
factor
study,
lower
being
harmful.
In
addition,
within
level,
element
induced
response
species.
This
provides
valuable
information
regarding
interaction
between
REEs
highlighting
importance
research
mitigating
pollution
climate
change
marine
ecosystems.
Analytical and Bioanalytical Chemistry,
Год журнала:
2022,
Номер
414(19), С. 5877 - 5886
Опубликована: Июнь 4, 2022
Non-steroidal
anti-inflammatory
drugs
(NSAIDs)
have
been
reported
to
be
the
main
pharmaceutical
class
accumulated
in
seafood.
Among
them,
ibuprofen
(IBU)
is
of
special
concern
as
it
used
worldwide
treat
common
pain,
does
not
require
a
medical
prescription,
often
taken
high
daily
dose,
and
has
cause
potential
adverse
effects
on
aquatic
organisms.
IBU
highly
transformed
into
hydroxy-
carboxy-metabolites
and/or
degradation
products
generated
only
after
its
administration
but
also
during
wastewater
treatment
or
environment.
These
compounds
can
present
environment
at
higher
concentrations
than
toxicity.
In
this
work,
low-cost
affordable
routine
analytical
method
was
developed
validated
for
first-time
determination
metabolites
mussels.
The
based
ultrasound-assisted
extraction
(UAE),
clean-up
by
dispersive
solid-phase
(d-SPE)
liquid
chromatography-tandem
mass
spectrometry.
Box-Behnken
experimental
design
optimisation
better
evaluate
influence
interactions
UAE
d-SPE
variables.
Extraction
recoveries
were
range
from
81
115%.
Precision,
expressed
relative
standard
deviation,
lower
7%.
Method
detection
limits
0.1
1.9
ng
g-1
dry
weight.
successfully
applied
wild
