The Science of The Total Environment, Год журнала: 2021, Номер 796, С. 148887 - 148887
Опубликована: Июль 7, 2021
Язык: Английский
The Science of The Total Environment, Год журнала: 2021, Номер 775, С. 145790 - 145790
Опубликована: Фев. 15, 2021
Язык: Английский
Процитировано
325
The Science of The Total Environment, Год журнала: 2021, Номер 805, С. 149877 - 149877
Опубликована: Авг. 25, 2021
Wastewater surveillance for pathogens using reverse transcription-polymerase chain reaction (RT-PCR) is an effective and resource-efficient tool gathering community-level public health information, including the incidence of coronavirus disease-19 (COVID-19). Surveillance Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) in wastewater can potentially provide early warning signal COVID-19 infections a community. The capacity world's environmental microbiology virology laboratories SARS-CoV-2 RNA characterization increasing rapidly. However, there are no standardized protocols or harmonized quality assurance control (QA/QC) procedures surveillance. This paper technical review factors that cause false-positive false-negative errors wastewater, culminating recommended strategies be implemented to identify mitigate some these errors. Recommendations include stringent QA/QC measures, representative sampling approaches, virus concentration efficient extraction, PCR inhibition assessment, inclusion sample processing controls, considerations RT-PCR assay selection data interpretation. Clear interpretation guidelines (e.g., determination positive negative samples) critical, particularly when low. Corrective confirmatory actions must place inconclusive results diverging from current trends initial onset reemergence community). It also prudent perform interlaboratory comparisons ensure results' reliability interpretability prospective retrospective analyses. this aim improve detection applications. A silver lining pandemic efficacy continues demonstrated during global crisis. In future, should play important role range other communicable diseases.
Язык: Английский
Процитировано
230
Environmental Science Water Research & Technology, Год журнала: 2021, Номер 7(3), С. 504 - 520
Опубликована: Янв. 1, 2021
The reproducibility and sensitivity of 36 methods for quantifying the genetic signal SARS-CoV-2 in wastewater was evaluated a nationwide interlaboratory assessment U.S.
Язык: Английский
Процитировано
226
The Science of The Total Environment, Год журнала: 2021, Номер 804, С. 150060 - 150060
Опубликована: Сен. 8, 2021
Successful detection of SARS-COV-2 in wastewater suggests the potential utility wastewater-based epidemiology (WBE) for COVID-19 community surveillance. This systematic review aims to assess performance surveillance as early warning system transmission. A search was conducted PubMed, Medline, Embase and WBE Consortium Registry according PRISMA guidelines relevant articles published until 31st July 2021. Relevant data were extracted summarized. Quality each paper assessed using an assessment tool adapted from Bilotta et al.'s environmental science. Of 763 studies identified, 92 distributed across 34 countries shortlisted qualitative synthesis. total 26,197 samples collected between January 2020 May 2021 various locations serving population ranging 321 11,400,000 inhabitants. Overall sample positivity moderate at 29.2% all examined settings with spike (S) gene having maximum rate positive detections nucleocapsid (N) being most targeted. Wastewater signals preceded confirmed cases by up 63 days, 13 reporting before first detected community. At least 50 reported association viral load cases. While cannot replace large-scale diagnostic testing, it can complement clinical providing signs transmission more active public health responses. However, standardized validated methods are required along risk analysis modelling understand dynamics outbreaks.
Язык: Английский
Процитировано
187
Chemical Engineering Journal, Год журнала: 2021, Номер 415, С. 129039 - 129039
Опубликована: Фев. 21, 2021
Язык: Английский
Процитировано
174
Environmental Research, Год журнала: 2021, Номер 200, С. 111374 - 111374
Опубликована: Май 29, 2021
Язык: Английский
Процитировано
167
The Science of The Total Environment, Год журнала: 2021, Номер 774, С. 145608 - 145608
Опубликована: Фев. 9, 2021
Raw municipal wastewater from five treatment plants representing the vast majority of Qatar population was sampled between third week June 2020 and end August 2020, during period declining cases after peak first wave infection in May 2020. The N1 region SARS-CoV-2 genome used to quantify viral load using RT-qPCR. trend Ct values samples mirrored number new daily positive officially reported for country, confirmed by RT-qPCR testing naso-pharyngeal swabs. RNA detected 100% influent (7889 ± 1421 copy/L - 542,056 25,775 copy/L, based on assay). A mathematical model wastewater-based epidemiology developed estimate people infected with COVID-19 samples. estimated any given day approach declined 542,313 51,159 31,181 3081 over course sampling period, which significantly higher than numbers. However, seroprevalence data indicates that diagnosed infections represented only about 10% actual cases. estimates were lower corrected numbers application a static diagnosis ratio identified cases, is assumed be due difficulty quantifying losses as term. these results indicate presented WBE modeling allows realistic assessment incidence population, more reliable estimation at point time can achieved human biomonitoring alone.
Язык: Английский
Процитировано
148
The Science of The Total Environment, Год журнала: 2021, Номер 774, С. 145721 - 145721
Опубликована: Фев. 9, 2021
Язык: Английский
Процитировано
119
The Science of The Total Environment, Год журнала: 2022, Номер 820, С. 153290 - 153290
Опубликована: Янв. 20, 2022
Язык: Английский
Процитировано
90
The Science of The Total Environment, Год журнала: 2023, Номер 878, С. 162953 - 162953
Опубликована: Март 21, 2023
Язык: Английский
Процитировано
65