Monitoring of SARS-CoV-2 RNA in wastewater as an epidemiological surveillance tool in Mendoza, Argentina DOI Open Access
Maximiliano Giraud‐Billoud,

Paula Cuervo,

Jorgelina C. Altamirano

и другие.

The Science of The Total Environment, Год журнала: 2021, Номер 796, С. 148887 - 148887

Опубликована: Июль 7, 2021

Язык: Английский

Correlation of SARS-CoV-2 RNA in wastewater with COVID-19 disease burden in sewersheds DOI Creative Commons
Jennifer Weidhaas, Zachary T. Aanderud, D. Keith Roper

и другие.

The Science of The Total Environment, Год журнала: 2021, Номер 775, С. 145790 - 145790

Опубликована: Фев. 15, 2021

Язык: Английский

Процитировано

325

Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance DOI Creative Commons
Warish Ahmed, Stuart L. Simpson, Paul M. Bertsch

и другие.

The Science of The Total Environment, Год журнала: 2021, Номер 805, С. 149877 - 149877

Опубликована: Авг. 25, 2021

Wastewater surveillance for pathogens using reverse transcription-polymerase chain reaction (RT-PCR) is an effective and resource-efficient tool gathering community-level public health information, including the incidence of coronavirus disease-19 (COVID-19). Surveillance Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) in wastewater can potentially provide early warning signal COVID-19 infections a community. The capacity world's environmental microbiology virology laboratories SARS-CoV-2 RNA characterization increasing rapidly. However, there are no standardized protocols or harmonized quality assurance control (QA/QC) procedures surveillance. This paper technical review factors that cause false-positive false-negative errors wastewater, culminating recommended strategies be implemented to identify mitigate some these errors. Recommendations include stringent QA/QC measures, representative sampling approaches, virus concentration efficient extraction, PCR inhibition assessment, inclusion sample processing controls, considerations RT-PCR assay selection data interpretation. Clear interpretation guidelines (e.g., determination positive negative samples) critical, particularly when low. Corrective confirmatory actions must place inconclusive results diverging from current trends initial onset reemergence community). It also prudent perform interlaboratory comparisons ensure results' reliability interpretability prospective retrospective analyses. this aim improve detection applications. A silver lining pandemic efficacy continues demonstrated during global crisis. In future, should play important role range other communicable diseases.

Язык: Английский

Процитировано

230

Reproducibility and sensitivity of 36 methods to quantify the SARS-CoV-2 genetic signal in raw wastewater: findings from an interlaboratory methods evaluation in the U.S. DOI Creative Commons

Brian M. Pecson,

Emily Darby, Charles N. Haas

и другие.

Environmental Science Water Research & Technology, Год журнала: 2021, Номер 7(3), С. 504 - 520

Опубликована: Янв. 1, 2021

The reproducibility and sensitivity of 36 methods for quantifying the genetic signal SARS-CoV-2 in wastewater was evaluated a nationwide interlaboratory assessment U.S.

Язык: Английский

Процитировано

226

Wastewater surveillance to infer COVID-19 transmission: A systematic review DOI Creative Commons
Shimoni Shah, Sylvia Xiao Wei Gwee,

Jamie Qiao Xin Ng

и другие.

The Science of The Total Environment, Год журнала: 2021, Номер 804, С. 150060 - 150060

Опубликована: Сен. 8, 2021

Successful detection of SARS-COV-2 in wastewater suggests the potential utility wastewater-based epidemiology (WBE) for COVID-19 community surveillance. This systematic review aims to assess performance surveillance as early warning system transmission. A search was conducted PubMed, Medline, Embase and WBE Consortium Registry according PRISMA guidelines relevant articles published until 31st July 2021. Relevant data were extracted summarized. Quality each paper assessed using an assessment tool adapted from Bilotta et al.'s environmental science. Of 763 studies identified, 92 distributed across 34 countries shortlisted qualitative synthesis. total 26,197 samples collected between January 2020 May 2021 various locations serving population ranging 321 11,400,000 inhabitants. Overall sample positivity moderate at 29.2% all examined settings with spike (S) gene having maximum rate positive detections nucleocapsid (N) being most targeted. Wastewater signals preceded confirmed cases by up 63 days, 13 reporting before first detected community. At least 50 reported association viral load cases. While cannot replace large-scale diagnostic testing, it can complement clinical providing signs transmission more active public health responses. However, standardized validated methods are required along risk analysis modelling understand dynamics outbreaks.

Язык: Английский

Процитировано

187

Uncertainties in estimating SARS-CoV-2 prevalence by wastewater-based epidemiology DOI Open Access
Xuan Li, Shuxin Zhang, Jiahua Shi

и другие.

Chemical Engineering Journal, Год журнала: 2021, Номер 415, С. 129039 - 129039

Опубликована: Фев. 21, 2021

Язык: Английский

Процитировано

174

Targeted wastewater surveillance of SARS-CoV-2 on a university campus for COVID-19 outbreak detection and mitigation DOI Open Access
Laura C. Scott, Alexandra Aubee, Layla Babahaji

и другие.

Environmental Research, Год журнала: 2021, Номер 200, С. 111374 - 111374

Опубликована: Май 29, 2021

Язык: Английский

Процитировано

167

COVID-19 (SARS-CoV-2) outbreak monitoring using wastewater-based epidemiology in Qatar DOI Creative Commons
Jayaprakash Saththasivam,

Shimaa S. El-Malah,

Tricia Gomez

и другие.

The Science of The Total Environment, Год журнала: 2021, Номер 774, С. 145608 - 145608

Опубликована: Фев. 9, 2021

Raw municipal wastewater from five treatment plants representing the vast majority of Qatar population was sampled between third week June 2020 and end August 2020, during period declining cases after peak first wave infection in May 2020. The N1 region SARS-CoV-2 genome used to quantify viral load using RT-qPCR. trend Ct values samples mirrored number new daily positive officially reported for country, confirmed by RT-qPCR testing naso-pharyngeal swabs. RNA detected 100% influent (7889 ± 1421 copy/L - 542,056 25,775 copy/L, based on assay). A mathematical model wastewater-based epidemiology developed estimate people infected with COVID-19 samples. estimated any given day approach declined 542,313 51,159 31,181 3081 over course sampling period, which significantly higher than numbers. However, seroprevalence data indicates that diagnosed infections represented only about 10% actual cases. estimates were lower corrected numbers application a static diagnosis ratio identified cases, is assumed be due difficulty quantifying losses as term. these results indicate presented WBE modeling allows realistic assessment incidence population, more reliable estimation at point time can achieved human biomonitoring alone.

Язык: Английский

Процитировано

148

A critical review on SARS-CoV-2 infectivity in water and wastewater. What do we know? DOI Open Access
Alexandre Giacobbo, Marco Antônio Siqueira Rodrigues, Jane Zoppas Ferreira

и другие.

The Science of The Total Environment, Год журнала: 2021, Номер 774, С. 145721 - 145721

Опубликована: Фев. 9, 2021

Язык: Английский

Процитировано

119

Factors influencing SARS-CoV-2 RNA concentrations in wastewater up to the sampling stage: A systematic review DOI Open Access
Xander Bertels,

Phaedra Demeyer,

Siel Van den Bogaert

и другие.

The Science of The Total Environment, Год журнала: 2022, Номер 820, С. 153290 - 153290

Опубликована: Янв. 20, 2022

Язык: Английский

Процитировано

90

Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling DOI Open Access
Stéfano Ciannella, Cristina González-Fernández, Jenifer Gómez‐Pastora

и другие.

The Science of The Total Environment, Год журнала: 2023, Номер 878, С. 162953 - 162953

Опубликована: Март 21, 2023

Язык: Английский

Процитировано

65