Pharmaceutical Development and Technology,
Год журнала:
2024,
Номер
29(2), С. 123 - 130
Опубликована: Фев. 7, 2024
This
study
was
to
construct
a
nanovesicle
delivery
system
improve
the
loading
efficiency
and
stability
of
ORI
for
treatment
nonalcoholic
fatty
liver
disease
(NAFLD).
nanovesicles
(NVs)
exerted
narrow
size
distribution
(195.6
±
11.49
nm)
high
entrapment
(84.46
1.34%).
In
vitro
cell
studies
demonstrated
that
NVs
enhanced
cellular
uptake
reduced
lipid
over-accumulation
total
cholesterol
levels
in
NAFLD
model.
At
same
time,
vivo
proved
that,
compared
with
normal
group,
model
group
mice
showed
decrease
body
weight,
significant
increase
index
(6.71
0.62,
p
<
0.01),
symptoms
accumulation,
vesicles,
tissue
fibrosis.
Compared
after
high-dose
intervention,
gained
decreased
(4.69
0.55,
hepatic
droplet
vacuoles,
accumulation
(reduced
oil
red
area,
0.001),
alleviated
degree
fibrosis
blue
collagen
0.001).
conclusion,
ORI/HP-β-CD/H9-HePC
specific
improved
therapeutic
effects,
nano
drug
provides
promising
strategy
encapsulation
effectively
alleviate
process
NAFLD.
Environmental Toxicology,
Год журнала:
2023,
Номер
38(10), С. 2416 - 2428
Опубликована: Июнь 22, 2023
Abstract
The
environmental
pollutant
bisphenol
A
(BPA),
used
in
the
manufacture
of
plastic
packaging
materials
for
various
diets,
is
widely
distributed
environment
and
causes
severe
hepatotoxicity
by
inducing
oxidative
stress.
Artemisia
argyi
essential
oil
(AAEO),
a
volatile
component
isolated
from
H.Lév
.
&
Vaniot
,
has
pharmacological
effects,
especially
hepatoprotective
actions.
However,
potential
effect
AAEO
BPA
induced
not
been
characterized.
First,
we
analyzed
chemical
composition
gas
chromatography–mass
spectrometry.
Herein,
investigated
on
hepatic
metabolic
changes
mice
exposed
to
BPA.
Results
showed
that
compared
with
group,
could
reduce
level
liver
function
enzymes
serum,
ameliorate
lesions
fibrosis.
Additionally,
20
differential
metabolites
screened
metabolomics
were
mainly
involved
reprogramming
glutathione
metabolism,
purine
polyunsaturated
fatty
acid
synthesis.
Moreover,
ferroptosis
BPA,
as
demonstrated
reducing
xanthine
oxidase
activity,
up‐regulating
activities
peroxidase
4
(GPX4),
superoxide
dismutase,
catalase
expression
SLC7A11
promote
synthetic,
while
inhibiting
transferrin
receptor
1
(TFR1)
accumulation
Fe
2+
cells.
Therefore,
our
study
identified
protectant
against
BPA‐induced
reversing
occurrence
ferroptosis.
Chemical Research in Toxicology,
Год журнала:
2021,
Номер
34(6), С. 1578 - 1587
Опубликована: Май 21, 2021
Toxic
effects
induced
upon
exposure
to
low-dose
bisphenol
A
(BPA)
or
S
(BPS)
remains
controversial.
In
this
study,
metabolomics
was
used
examine
the
metabolomic
perturbation
arising
from
28
days
of
BPA
BPS
at
50
μg/kg
bw/day
in
Sprague–Dawley
(SD)
rats.
Endogenous
metabolite
profiling
revealed
a
clear
discrimination
metabolome
rat
plasma
among
BPA-treatment,
BPS-treatment,
and
control
groups.
up-regulation
19
metabolites
down-regulation
32
SD
rats,
compared
with
control.
15
33
Joint
pathway
analysis
suggested
marked
perturbations
citrate
cycle,
butanoate
metabolism,
alanine,
aspartate,
glutamate
metabolism
for
BPA-exposed
rats
as
well
glycerophospholipid
BPS-exposed
These
findings
provide
novel
insights
into
associations
between
phenotypic
changes
exposure.
Pakistan Veterinary Journal,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Bisphenol
A
(BPA)
has
several
potential
uses,
including
in
polycarbonate
plastics
and
epoxy
resins,
which
could
expose
humans
to
it.Recognized
for
its
hepatotoxicity
ability
accumulate
organs.We
prompted
this
study
explore
the
hepatoprotective
of
astaxanthin
(ASTX),
an
antioxidant
against
BPA
toxicity.We
used
32
male
Wistar
Albino
rats
randomly
assigned
them
as:
Control,
Sham
(olive
oil),
BPA,
BPA+ASTX.At
end
experiment,
Native
Thiol,
Total
alanine
aminotransferase
(ALT),
aspartate
(AST)
were
measured
serum
samples.Histopathological
scoring
was
performed
evaluate
changes
caused
by
ASTX
liver.Caspase
3
caspase
9
expression
liver
tissues
demonstrated
immunohistochemically
PCR.Collagen
I
(COL1A1)
collagen
III
(COL3A1)
mRNA
levels
PCR
tissue
samples.The
group
showed
elevated
AST
ALT
with
decreased
Thiol
levels.ASTX
administration
reversed
these
as
observed
reduced
increased
levels.Histopathology
indicated
damage
fibrosis
alleviated
BPA+ASTX
group.Gene
analyses
revealed
upregulated
COL1A1
COL3A1
downregulated
ASTX.Immunohistochemistry
confirmed
BPA-induced
expression,
attenuated
ASTX.This
underscores
ASTX's
efficacy
ultimately
attributed
antiapoptotic
properties.Consequently,
emerges
a
promising
therapeutic
agent
preventing
treating
BPA-related
diseases.
Cells,
Год журнала:
2021,
Номер
10(9), С. 2243 - 2243
Опубликована: Авг. 30, 2021
Uric
acid
(UA)
is
the
end-product
in
human
purine
metabolism
pathway.
The
UA
that
accumulates
silkworm
tissues
excreted
as
a
nitrogen
waste
product.
Here,
we
first
validated
Bombyx
mori
has
homolog
of
gene
encodes
5′-nucleotidase
(5′N)
involved
metabolism.
B.
gene,
Bm5′N,
located
upstream
other
genes
silkworm.
Disruption
Bm5′N
via
CRISPR/Cas9
system
resulted
decreased
levels
epidermis
and
caused
translucent
skin
phenotype.
When
mutant
silkworms
were
fed
with
uric
precursor
inosine,
increased
significantly.
Furthermore,
metabolomic
transcriptomic
analyses
mutants
indicated
loss
affected
ABC
transport
Taken
together,
these
results
suggest
pathway
conserved
between
humans
plays
crucial
role
Thus,
may
be
suitable
model
for
study
pathways
relevant
to
disease.
